Soil piping is a widespread land degradation process that may lead to gully formation. However, the processes involved in sediment detachment from soil pipe walls have not been well studied, although their recognition is a crucial step to protect soils from piping erosion. This study aims to recognize the factors affecting cohesion and to identify the mechanisms which are likely to be responsible for the disintegration of soil. The study has been conducted in mid-altitude mountains under a temperate climate (the Bieszczady Mountains, the Carpathians, SE Poland). The research was based on the detailed field and laboratory analyses of morphology, and the physical and chemical properties of soil profiles with and without soil pipes. Moreover, experiments with flooding the undisturbed soil samples using different solutions (deionized water, ammonium oxalate, dithionate citrate, 35% hydrochloric acid and 30% hydrogen peroxide) were conducted in order to check the role of air slaking, the removal of soil organic carbon (SOC), and Fe and Al oxides on sediment detachment. The obtained results have confirmed that soil pipes develop in quite cohesive soils (silt loams), which allow the formation and maintenance of pipes with a diameter up to 30cm. Soil cohesion, and thus susceptibility to piping, are impacted by the content of major oxides, soil particle size distribution, biological activity and porosity. The tested soils affected by piping erosion have a lower content of Al 2 O 3 and Fe 2 O 3 , and free Fe (Fe (DCB)), lower clay content, higher biological activity (more roots and animal burrows), higher porosity, and more and larger pores than the profile without soil pipes. The experiments have indicated that especially SOC along with Fe and Al oxides are an important cohesion source in the study area. This suggests that the removal of SOC, and Fe and Al oxides may weaken and disintegrate aggregates in soil pipes. Further study of soil leaching and tensile strength will broaden understanding of which chemical processes control where pipes will develop in other cohesive piping-prone soils.