+92-0334-6518990, e-mail: anwer_sumera@yahoo.com; Dr.Naeem Iqbal: +92-0300-7979767, email: drnaeem@gcuf.edu.pk; Dr.Abida Kausar: +92-0322-6393121, e-mail: kausaragc@yahoo.com; Mohsan Ali: +92-0306-7200721, e-mail: mohsinrashid242@gmail.com; Ali Nouman: +92-0300-6694992, e-mail: alinoman@gcuf.edu.pk; Muhammad Amir Zafar: +92 -0335-1867207, e-mail: amir.zafar55@yahoo.com; Komal Feroz:e-mail: komalferoz@ymail.com) Abstract. The study was conducted to investigate the impact of various concentration of domestic waste water on growth, physiological, and biochemical characteristics of tomato, okra and pumpkin. Sewage water was applied of 50% and 100% on vegetables grown under field conditions. Irrigation with tubewell water was considered as control. At maturity data for growth attributes was recorded. Maximum fresh weight of root, shoot and fruit was in tomato at 50% sewage water treatment followed by control. Dry weight also exhibited a considerable increase at 50% sewage water application. However, a decline in chlorophyll a, b and carotenoid contents was observed at 100% sewage water. Antioxidants and secondary metabolites increased and their maximum values were recorded at 100% sewage treatment in all the three vegetables.