Assessment of immune response in periparturient dairy cows using ex vivo whole blood stimulation assay with lipopolysaccharides and carrageenan skin test

Jahan, Nusrat; Minuti, Andrea; Trevisi, Erminio

doi:10.1016/j.vetimm.2015.04.003

Cited by 42 publications

(44 citation statements)

References 40 publications

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“…The plasma concentrations of IL‐6 and TNF‐ α in this study were similar to previous measurements in dairy cows (Ishikawa et al., ; Rodriguez‐Sallaberry et al., ; Kasimanickam et al., ; Jahan et al., ; Trevisi et al., ). Nevertheless, the variation of PIC was unexpected at the end of the clamps because generally changes in these mediators are considered to occur quickly after the induction of their release and are short‐lived.…”

Section: Discussionsupporting

confidence: 90%

Acute phase response in lactating dairy cows during hyperinsulinemic hypoglycaemic and hyperinsulinemic euglycaemic clamps and after intramammaryLPSchallenge

Matteis

Bertoni

Lombardelli

et al. 2016

Animal Physiology Nutrition

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The link between energy availability, turnover of energy substrates and the onset of inflammation in dairy cows is complex and poorly investigated. To clarify this, plasma inflammatory variables were measured in mid-lactating dairy cows allocated to three groups: hyperinsulinemic hypoglycaemic clamp, induced by insulin infusion (HypoG, n = 5); hyperinsulinemic euglycaemic clamp, induced by insulin and glucose infusion (EuG; n = 6); control, receiving a saline solution infusion (NaCl; n = 6). At 48 h after the start of i.v. infusions, two udder quarters per cow were challenged with 200 μg of E. coli lipopolysaccharide (LPS). Individual blood samples were taken before clamps, before LPS challenge (i.e. 48 h after clamps) and 6.5 h after. At 48 h, positive acute phase proteins (posAPP) did not differ among groups, whereas albumin and cholesterol (index of lipoproteins), negative APP (negAPP), were lower (p < 0.05) in EuG compared to NaCl and HypoG. The concentration of IL-6 was greater in EuG (p < 0.05) but only vs. HypoG. At 6.5 h following LPS challenge, IL-6 increased in the NaCl and EuG clamps (p < 0.05), while TNF-α increased (p < 0.05) in the EuG only. Among the posAPP, haptoglobin markedly increased in EuG (p < 0.05), but not in NaCl (p = 0.76) and in HypoG; ceruloplasmin tended to decline during LPS challenge, the reduction was significant when all animals were considered (p < 0.05). Conversely, all the negAPP showed a marked reduction 6.5 h after LPS challenge in the three groups. In conclusion, EuG caused an inflammatory status after 48-h infusion (i.e. decrease of negAPP) and induced a quicker acute phase response (e.g. marked rise of TNF-α, IL-6) after the intramammary LPS challenge. These data suggest that the simultaneous high availability of glucose and insulin at the tissue-level makes dairy cows more susceptible to inflammatory events. In contrast, HypoG seems to attenuate the inflammatory response.

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Section: Discussionsupporting

confidence: 90%

Acute phase response in lactating dairy cows during hyperinsulinemic hypoglycaemic and hyperinsulinemic euglycaemic clamps and after intramammaryLPSchallenge

Matteis

Bertoni

Lombardelli

et al. 2016

Animal Physiology Nutrition

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“…Whereas 88.2% of the first Imrestor injections occurred within a 2-wk window before the actual calving date, 11.8% of the first Imrestor injections were applied outside the ideal timeline. As the latter group would not necessarily be experiencing the immune suppression around calving time (Jahan et al, 2015), it is important to emphasize the necessity to improve the prediction of the calving date. Based on our field experience obtained during this trial, we recommend observing cows twice weekly for clinical signs of parturition to target the first Imrestor dose according to label recommendations (7 d before expected parturition).…”

Section: Discussionmentioning

confidence: 99%

“…Lastly, the current study only focused on monitoring some immune-related disorders (mastitis, RP, and metritis) during the first 30 DIM. Although the immune suppression of the dairy cows may start before the energy deficiency (Grummer, 1995;Kimura et al, 1999;Bertoni et al, 2009;Jahan et al, 2015) the interaction between negative energy balance, metabolic and immune diseases, and hypocalcemia is well documented (Kimura et al, 2006;Esposito et al, 2014;Ingvartsen and Moyes, 2015), and the potential benefits of Imrestor on other diseases will need to be further investigated.…”

Section: Discussionmentioning

confidence: 99%

“…The metabolic challenge around parturition is such that a dairy cow may enter into a negative energy balance, even before calving and without milk being produced (Grummer, 1995). On the other hand, it is well documented that effects of the parturition itself impair the immune response of the dairy cow (Mallard et al, 2009), and that such immune suppression may start up to 21 d before calving (Kimura et al, 1999) or only a few days before calving (Jahan et al, 2015). The effect and consequences of the negative energy balance and immune suppression around parturition are shown in Figure 1.…”

Section: Introductionmentioning

confidence: 99%

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Investigation of the effect of pegbovigrastim on some periparturient immune disorders and performance in Mexican dairy herds

Ruiz

Tedeschi

Sepúlveda

2017

Journal of Dairy Science

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This study analyzed the efficacy of pegbovigrastim (Imrestor, Elanco Animal Health, Greenfield, IN) on some health and production parameters in lactating dairy cows. Primiparous and multiparous Holsteins from 17 Mexican dairy herds (n = 10,238) were included in this study, which was conducted in 2015. Treated cows (n = 5,025) received Imrestor approximately 7 d before expected calving and again within 24 h after calving; control cows (n = 5,213) did not receive a placebo. Clinical mastitis (MAS; 0 to 30 DIM), retained placenta (RP), and clinical metritis (MET; 0 to 21 DIM) occurrences were recorded, as well as the number of medication days, medical treatments needed, and the number of days that milk was discarded due to medication administered for disease. Milk yield was measured from calving until 120 d in milk. Imrestor reduced the incidence of MAS by 25%, and the odds ratio of having mastitis in the first 30 d in milk was 35% greater for control cows than for Imrestor cows. Imrestor treatment reduced the number of medical treatments required for MAS by 6%, and less milk was discarded due to medication for MAS as a result of the Imrestor treatment. The incidence of RP was reduced by 4.15% with Imrestor and the odds of cows having RP were 4.6% greater for control than Imrestor treatments, but they did not differ. The MET incidence was increased by 17.1% with Imrestor. The use of Imrestor around parturition increased by 5.8% the odds of inseminating cows during the first 100 d after calving. Imrestor-treated multiparous cows with MAS produced 2.1 kg/d more milk than control cows with MAS during the first 30 d of their lactation. Imrestor-treated multiparous cows with MET produced 2.3 kg/d more milk than MET control cows during the first 120 d of their lactation. We conclude that Imrestor can help the dairy cow cope with immune periparturient disorders and can increase the milk yield of dairy cows due to a healthier transition, despite a reported increase in the incidence of MET.

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“…The use of animal disease models allows researchers not only to investigate the pathogenesis of various diseases but also to test new therapeutic agents (Choi et al, ). Carrageenan is a chemical that is widely used to induce acute inflammation in animal models (Cicala et al, ; Fecho, Nackley, Wu, & Maixner, ; Guay, Bateman, Gordon, Mancini, & Riendeau, ; Huang et al, ; Jahan, Minuti, & Trevisi, ; Posadas et al, ; Xu et al, ). It is able to rapidly induce edema and has a strong effect on behavior (Fehrenbacher, Vasko, & Duarte, ), but the duration of the response is very short.…”

Section: Introductionmentioning

confidence: 99%

Label‐free monitoring of inflammatory tissue conditions using a carrageenan‐induced acute inflammation rat model

Lee

Shin

et al. 2018

Microscopy Res & Technique

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Although the confirmation of inflammatory changes within tissues at the onset of various diseases is critical for the early detection of disease and selection of appropriate treatment, most therapies are based on complex and time-consuming diagnostic procedures. Raman spectroscopy has the ability to provide non-invasive, real-time, chemical bonding analysis through the inelastic scattering of photons. In this study, we evaluate the feasibility of Raman spectroscopy as a new, easy, fast, and accurate diagnostic method to support diagnostic decisions. The molecular changes in carrageenan-induced acute inflammation rat tissues were assessed by Raman spectroscopy. Volumes of 0 (control), 100, 150, and 200 µL of 1% carrageenan were administered to rat hind paws to control the degree of inflammation. The prominent peaks at [1,062, 1,131] cm and [2,847, 2,881] cm were selected as characteristic measurements corresponding to the C-C stretching vibrational modes and the symmetric and asymmetric C-H (CH ) stretching vibrational modes, respectively. Principal component analysis of the inflammatory Raman spectra enabled graphical representation of the degree of inflammation through principal component loading profiles of inflammatory tissues on a two-dimensional plot. Therefore, Raman spectroscopy with multivariate statistical analysis represents a promising method for detecting biomolecular responses based on different types of inflammatory tissues.

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Assessment of immune response in periparturient dairy cows using ex vivo whole blood stimulation assay with lipopolysaccharides and carrageenan skin test

Cited by 42 publications

References 40 publications

Acute phase response in lactating dairy cows during hyperinsulinemic hypoglycaemic and hyperinsulinemic euglycaemic clamps and after intramammaryLPSchallenge

Acute phase response in lactating dairy cows during hyperinsulinemic hypoglycaemic and hyperinsulinemic euglycaemic clamps and after intramammaryLPSchallenge

Investigation of the effect of pegbovigrastim on some periparturient immune disorders and performance in Mexican dairy herds

Label‐free monitoring of inflammatory tissue conditions using a carrageenan‐induced acute inflammation rat model

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