Assessment of immunochromatographic test for rapid lymphatic filariasis diagnosis

Nguyễn, Ngọc Tuấn; Plichart, Catherine; Esterre, P.

doi:10.1051/parasite/1999064355

Cited by 24 publications

(15 citation statements)

References 10 publications

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“…In case of positive results with the ICT assay, a reference ELISA-based tech nique (Og4c3 kit, JCU Tropical Biotech Ltd., Townsville, Australia) was used for confirmation (Nguyen et al, 1999). We also studied the antistreptococcal antibody responses by estimating the anti-streptolysin O (ASLO) titers with an immunoturbidimetric assay (Tina-Quant® ASLO, using an Hitachi 717 instrument, BoehringerMannheim, Mannheim, Germany), and the anti-streptodornase B (ASDB) with an enzymatic inhibition (ADNase B®, Dade Behring, Paris La Defense, France).…”

Section: Methodsmentioning

confidence: 99%

Role of streptococcal infection in the acute pathology of lymphatic filariasis

Esterre¹,

Plichart²,

Huin-Blondey³

et al. 2000

Parasite

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Summary:Growing evidence suggest that secondary bacterial, mainly streptococcal, infections contribute significantly to recurrent episodes of acute adenolymphangitis (ADL| of filarial origin. We examined the role of group A streptococci in the progression of lymphedema in Polynesian patients with filariasis-related ADL (22 cases) or chronic pathology (10 cases), or with erysipela (10 patients) and, as controls, in 20 healthy adults. Antistreptolysin O (ASLO) and anti-streptodornase B (ASDB) titers were systematically determined in parallel to parasitological and biochemical tests. ASLO and ASDB assays were positive in 100 % of erysipela, 75 % of filarial ADL as compared to 50 % of chronic pathology and 39 % of healthy controls. Interestingly, by opposition to ASLO titers which were not significantly different between the four groups, ASDB titers were higher in ADL (p = 0.019) and erysipela (p = 0.002) than in controls. These results support the hypothesis that recurrent streptococcal infections may have an important role in the pathogenesis of ADL in lymphatic filariasis.KEY WORDS : lymphatic filariasis, Wuchereri a bancrofti, group A streptococci, acute adenolymphangitis, French Polynesia. (Fan et al., 1995). The acute form of bancroftian filariasis involves lymphadenitis and lymphangitis, generally called adenolymphangitis (ADL) and is supposed to be, at least partly, a local immunological response to adult worms associated to mechanical damages of the lymphatic vessels made by these motile parasites. Repeated lymphatic damages related to adult worms activity is supposed to result in increased susceptibility to secondary bacterial infections, leading to progression of lymphoedema and elephantiasis (Olszewski et al., 1993(Olszewski et al., , 1994Schacher & Sahyoun, 1967;Shenoy et al, 1995 et al, 1998) associated morbi dity. The present study examined serologically infec tions with group AB hemolytic streptococci, one of the main common pathogenic bacteria in tropical areas with a marked affinity for the lymphatics, in ADL of filarial and bacterial (erysipela, see clinical definition in Chattier &Grosshans, 1990, andVincent et al, 1998) origins, compared to controls with the same eth nological and social conditions. MOTS CLES MATERIAL AND METHODS SERUM SAMPLESP rospective patients, examined by an experienced medical staff (Clinical Research Unit, Institut Malarde, Papeete, French Polynesia), were care fully questioned for a clinical history of recurrent epi sodes of ADL (Suma et al, 1997;Vincent et al, 1998). The selected population consisted of 22 patients with filarial ADL, 10 patients with chronic pathology (six hydroceles, two elephantiasis and two chyluria) and 10 with typical erysipelas and 20 endemic healthy controls of the same ethnological (Polynesian) and social (life-long residents of Tahiti island, Society archi pelago) conditions. After informed consent, diligent cli nical and laboratory investigations (complete hemogram, serum biochemistry and detection of microfilariae by Nucleopore® membrane fi...

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Section: Methodsmentioning

confidence: 99%

Role of streptococcal infection in the acute pathology of lymphatic filariasis

Esterre¹,

Plichart²,

Huin-Blondey³

et al. 2000

Parasite

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“…The immunochromatographic method (AMRAD ICT, New South Wales, Australia), specially developed for the diagnosis of bancroftian filariasis in human serum or plasma, has shown various advantages when compared to the TBF. Besides the simplicity of daytime blood collection, the ICT card test has demonstrated high sensitivity and specificity when performed in serum 2,8,11,13,16,22,26 .…”

Section: Introductionmentioning

confidence: 99%

Field evaluation of the whole blood immunochromatographic test for rapid bancroftian filariasis diagnosis in the northeast of Brazil

Braga

Dourado

Ximenes

et al. 2003

Rev. Inst. Med. trop. S. Paulo

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SUMMARYThis study evaluated the whole blood immunochromatographic card test (ICT card test) in a survey performed in Northeastern Brazil. 625 people were examined by the thick blood film (TBF) and ICT card test. Residents of a non-endemic area were also tested by the whole blood card test and Og4C3. The sensitivity of the ICT card test was 94.7% overall, but lower in females than males, based on the reasonable assumption that TBF is 100% specific. However, since TBF and other methods have unknown sensitivity, the true specificity of the card test is unknown. Nevertheless, it is possible to estimate upper and lower limits for the specificity, and relate it to the prevalence of the disease. In the endemic area, the possible range of the specificity was from 72.4% to 100%. 29.6% of the card tests performed in the non-endemic area exhibited faint lines that were interpreted as positives.Characteristics of the method including high sensitivity, promptness and simplicity justify its use for screening of filariasis. However, detailed information about the correct interpretation in case of extremely faint lines is essential. Further studies designed to consider problems arising from imperfect standards are necessary, as is a sounder diagnostic definition for the card test.

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“…The biological diagnostic was revolutionized by tests detecting circulating filarial antigens [15] in patients carrying adult worms, whether symptomatic or not, with or without microfilariae. Their detection is more sensitive than screening and does not depend on the periodicity of microfilariae circulation; ELISA (Og4C3) or rapid immunochromatographic tests (ICT cards) [16][17][18][19] are available. ICT tests are the most frequently used tests in the South Pacific and used routinely in FP.…”

Section: Introductionmentioning

confidence: 99%