Urban Heat Islands (UHI) consist of the occurrence of higher temperatures in urbanized areas when compared to rural areas. During the warmer seasons, this effect can lead to thermal discomfort, higher energy consumption, and aggravated pollution effects. The application of Remote Sensing (RS) data/techniques using thermal sensors onboard satellites, drones, or aircraft, allow for the estimation of Land Surface Temperature (LST). This article presents a systematic review of publications in Scopus and Web of Science (WOS) on UHI analysis using RS data/techniques and LST, from 2000 to 2020. The selection of articles considered keywords, title, abstract, and when deemed necessary, the full text. The process was conducted by two independent researchers and 579 articles, published in English, were selected. Qualitative and quantitative analyses were performed. Cfa climate areas are the most represented, as the Northern Hemisphere concentrates the most studied areas, especially in Asia (69.94%); Landsat products were the most applied to estimates LST (68.39%) and LULC (55.96%); ArcGIS (30.74%) was most used software for data treatment, and correlation (38.69%) was the most applied statistic technique. There is an increasing number of publications, especially from 2016, and the transversality of UHI studies corroborates the relevance of this topic.