Assessment of in vitro and in vivo recovery of gallamine using microdialysis

An assessment of calibration and performance of the microdialysis system

“…The microdialysis technique has been used for continuous sampling of low molecular weight compounds including glucose, lactate, pyruvate, glycerol, glutamate and urea, as well as for sampling of pharmacological agents in the extracellular space [1,3,5,6]. For many drugs and endogenous ligands, specific receptors are localized in peripheral compartments rather than in serum.…”

Assaying protein unbound drugs using microdialysis techniques

“…Comparison of the in vitro recovery method, by gain, loss and the zero-net-flux showed that the recovery of gallamine was within the ranges of 38.4-38.9%. In addition, the in vitro recovery before and after in vivo experiment was examined, indicating that the performance of microdialysis probe was stable after being implanted in the muscle tissue, as confirmed by the in vitro experiment performed directly after completion of the in vivo studies [79]. These results suggest that using microdialysis for the study of pharmacokinetics and pharmacodynamics is strongly recommended.…”

The percutaneous permeability and absorption of dexamethasone esters in diabetic rats: a preliminary study

“…It is well established that the quantity of a compound recovered by microdialysis is only a fraction of that present in the tissue (Leveque et al, 2004). The ratio of the concentration of analyte in microdialysate samples relative to the medium surrounding the probe is defined as recovery (Elmquist & Sawchuk, 1997;Sasongko et al, 2000). The actual concentration of drug in the probe-implanted location is demanded to calculate the recovery.…”