Assessment of in vitro anthelmintic activity and bio-guided chemical analysis of BRS Boyrá pineapple leaf extracts

Bioactive compounds from the parasitic plant Arceuthobium vaginatum inhibit Haemonchus contortus egg hatching

“…These results were similar to the findings of our study with the A. vaginatum EtOAc-E. In another study with an EtOAc-E and two fractions from Ananas comosus (Bromeliaceae), an ovicidal effect close to 100% at 5 mg/mL on H. contortus eggs was recorded (Rodrigues et al, 2020). In the present study, when the extract was fractionated, the subfraction AvR9 exhibited the same ovicidal effect at 0.25 mg/mL (Table 1).…”

“…Chlorogenic acid was isolated from Tagetes filiflora (Asteraceae) and showed 100% of EHI at 0.5 mg/mL against H. contortus ( Jasso Díaz et al, 2017 ). Another phenolic acid like coumaric acid also showed ovicidal and larvicidal effects on this parasite ( Castañeda-Ramírez et al, 2019 ; Mancilla-Montelongo et al, 2019 ; Rodrigues et al, 2020 ). In this regard, these chemical constituents present in A. vaginatum could be responsible for ovicidal activity.…”

“…Analyzing the EC 50 results of the present study, the AvR9 subfraction was 13.29, 10.34 and 1.36 more effective than the AvR7, AvR5 and AvR11 subfractions and the EtOAc-E. The A. vaginatum EtOAc-E was more active than those reported with an extract with the same polarity from Typha capensis (EC 50 = 0.12 vs 0.43 mg/mL) on H. contortus eggs ( Ondua et al, 2021 ) and Ananas comosus (EC 50 = 0.45 mg/mL) ( Rodrigues et al, 2020 ). In our study, the concentration required to cause a 50% of ovicidal effect from A. vaginatum EtOAc extract was only 0.12 mg/mL ( Table 2 ).…”

“…Some bioguided studies with plants rich in secondary metabolites have identified molecules like hydroxycinnamic acid and naringenin. For example, the coumaric acid has been isolated from A. comosus and was evaluated against H. contortus , demonstrating an important nematicidal effect ( Rodrigues et al, 2020 ). Thus, the ovicidal activity of AvR5 and AvR7 could be attributed to the hydroxycinnamic acid present in these fractions.…”

Moringa oleifera as a Natural Alternative for the Control of Gastrointestinal Parasites in Equines: A Review

“…In addition, antiparasitic drugs made from plants are considered biodegradable and ecological [88], thanks to the fact that they are less likely to accumulate in the tissues of animals, and in some cases, they contribute to the mitigation of greenhouse gases, which reduces the impact of animals on the environment [89,90]. These benefits are mainly attributed to the presence of bioactive compounds in plants, such as alkaloids, flavonoids, tannins, saponins, coumarins, terpenes, lignans, and amino acids, which have been reported to have antimethanogenic and antiparasitic properties [86,91,92]. In the case of antiparasitic properties, it has been reported that the same plant species can present several mechanisms of action against parasites because they present a wide variety of bioactive compounds, which also decreases the probability that parasites develop antiparasitic resistance [93].…”