Assessment of In Vitro Skin Irritation Potential of Nanoparticles: RHE Model

Murthy, P. Balakrishna; Kishore, A. Sairam; Surekha, P.

doi:10.1007/978-1-62703-002-1_16

Cited by 5 publications

(1 citation statement)

References 3 publications

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“…However, the growing ethical recognition of animal welfare has prompted the replacement of in vitro testing of skin corrosion and irritation using animals by alternative methods (3). Regulatory classifications of chemicals with skin corrosion and/or irritation are transitioning from using in vivo data to in vitro data obtained from alternative methods using human skin models (4).…”

Section: Introductionmentioning

confidence: 99%

Skin Corrosion and Irritation Test of Nanoparticles Using Reconstructed Three-Dimensional Human Skin Model, EpiDerm^TM

et al. 2016

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Effects of nanoparticles (NPs) on skin corrosion and irritation using three-dimensional human skin models were investigated based on the test guidelines of Organization for Economic Co-operation and Development (OECD TG431 and TG439). EpiDermTM skin was incubated with NPs including those harboring iron (FeNPs), aluminum oxide (AlNPs), titanium oxide (TNPs), and silver (AgNPs) for a defined time according to the test guidelines. Cell viabilities of EpiDermTM skins were measured by the 3-(4, 5-dimethylthi-azol-2-yl)-2.5-diphenyltetrazolium bromide based method. FeNPs, AlNPs, TNPs, and AgNPs were non-corrosive because the viability was more than 50% after 3 min exposure and more than 15% after 60 min exposure, which are the non-corrosive criteria. All NPs were also non-irritants, based on viability exceeding 50% after 60 min exposure and 42 hr post-incubation. Release of interleukin 1-alpha and histopathological analysis supported the cell viability results. These findings suggest that FeNPs, AlNPs, TNPs, and AgNPs are ‘non-corrosive’ and ‘non-irritant’ to human skin by a globally harmonized classification system.

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Section: Introductionmentioning

confidence: 99%

Skin Corrosion and Irritation Test of Nanoparticles Using Reconstructed Three-Dimensional Human Skin Model, EpiDerm^TM

et al. 2016

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Toxicology of Nanoparticles

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Nanocarriers for Drug Delivery

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Multimodal magnetic nanoparticles for biomedical applications: importance of characterization on biomimetic in vitro models

Pavlin

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et al. 2019

Materials for Biomedical Engineering

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Assessment of In Vitro Skin Irritation Potential of Nanoparticles: RHE Model

Abstract: The skin irritation test is designed for the prediction of acute skin irritation of nanoparticles by measurement of its cytotoxic effect, as reflected in the MTT assay, on the Reconstructed Human Epidermis (RHE) model. RHE tissues are commercially available.

Cited by 5 publications

References 3 publications

Skin Corrosion and Irritation Test of Nanoparticles Using Reconstructed Three-Dimensional Human Skin Model, EpiDerm^TM

Skin Corrosion and Irritation Test of Nanoparticles Using Reconstructed Three-Dimensional Human Skin Model, EpiDerm^TM

Toxicology of Nanoparticles

Multimodal magnetic nanoparticles for biomedical applications: importance of characterization on biomimetic in vitro models

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Assessment of In Vitro Skin Irritation Potential of Nanoparticles: RHE Model

Abstract: The skin irritation test is designed for the prediction of acute skin irritation of nanoparticles by measurement of its cytotoxic effect, as reflected in the MTT assay, on the Reconstructed Human Epidermis (RHE) model. RHE tissues are commercially available.

Cited by 5 publications

References 3 publications

Skin Corrosion and Irritation Test of Nanoparticles Using Reconstructed Three-Dimensional Human Skin Model, EpiDermTM

Skin Corrosion and Irritation Test of Nanoparticles Using Reconstructed Three-Dimensional Human Skin Model, EpiDermTM

Toxicology of Nanoparticles

Multimodal magnetic nanoparticles for biomedical applications: importance of characterization on biomimetic in vitro models

Contact Info

Product

Resources

About

Skin Corrosion and Irritation Test of Nanoparticles Using Reconstructed Three-Dimensional Human Skin Model, EpiDerm^TM

Skin Corrosion and Irritation Test of Nanoparticles Using Reconstructed Three-Dimensional Human Skin Model, EpiDerm^TM