Assessment of injection volume limits when using on-column focusing with microbore liquid chromatography

Mills, Malcolm J.; Maltas, John; Lough, W. J.

doi:10.1016/s0021-9673(96)00753-4

Cited by 91 publications

(42 citation statements)

References 23 publications

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“…To determine the retention coefficients used to calculate the theoretical maximum injection volume using the approach of Mills et al [18], a 0.27 g/mL strychnine standard was analyzed with an Agilent (Palo Alto, CA, USA) 1100 HPLC system with an ultra-violet multiple wavelength detector (MWD-UV), at λ = 254 nm. A 10 L sample was injected onto a Phenomenex (Torrence, CA, USA) Luna C-18(2), 5 m particle size, 150 mm × 2.0 mm column with a Phenomenex Luna C-18(2), 5 m particle size guard column.…”

Section: High Performance Liquid Chromatography On-column Focusing Omentioning

confidence: 99%

“…The mean retention (N = 3) coefficient for the sample solvent was 22.19 with a mean plate number of 338. A maximum injection volume of 73 L was calculated for the chromatography conditions to be used in the method using these values [18]. We elected to use a 50 L injection volume to allow for possible matrix interferences.…”

Section: Calculation Of Maximum Injection Volumementioning

confidence: 99%

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Development of a high performance liquid chromatography/mass spectroscopy method for the determination of strychnine concentrations in insects used to assess potential risks to insectivores

Stahl

ARJO²,

WAGNER³

et al. 2004

Journal of Chromatography B

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A high performance liquid chromatography/mass spectrometry (HPLC/MS) method was developed in support of a study to assess potential tertiary risks posed to insectivores by strychnine baited pocket gophers (Thomomys sp.). Necropholous insects are primary consumers of pocket gopher carcasses. A field study was conducted to collect insects from strychnine-baited and control pocket gopher carcasses. The majority of the insects collected were from the orders Diptera (flies, assayed separately as adults and larvae), Coleoptera (beetles), and Hymenoptera (ants and wasps, assayed separately). Samples (0.5 g) were extracted in acetic acid (2%) and analyzed with the mass spectrometer configured for tandem mass spectrometry. For most of the samples the strychnine concentrations were less than the method limit of detection. However, strychnine concentrations as high as 0.338, 0.341, 0.698, and 0.034 g/g were detected in ants, fly adults, fly larvae, and beetles, respectively. This information collected with the HPLC/MS method is critical for assessing potential non-target hazards for insectivores.

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Section: High Performance Liquid Chromatography On-column Focusing Omentioning

confidence: 99%

Section: Calculation Of Maximum Injection Volumementioning

confidence: 99%

Development of a high performance liquid chromatography/mass spectroscopy method for the determination of strychnine concentrations in insects used to assess potential risks to insectivores

Stahl

ARJO²,

WAGNER³

et al. 2004

Journal of Chromatography B

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“…This effect is taken to profit for analyzing components present in trace amounts in the sample. Indeed, very large sample volumes can then be introduced in the column, which leaves a sufficiently large amount of these trace analytes in the column for leading to high enough detection signal-to-noise ratios at the column outlet and precise enough quantitative analysis [27][28][29][30][31][32][33][34][35][36][37]. If, instead, the sample solvent is stronger than the mobile phase, the analyte migration velocity in the sample solvent is larger than in the mobile phase.…”

Section: Introductionmentioning

confidence: 99%

Influence of a strong sample solvent on analyte dispersion in chromatographic columns

Mishra

Rana

Wit

et al. 2013

Journal of Chromatography A

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a b s t r a c tIn chromatographic columns, when the eluting strength of the sample solvent is larger than that of the carrier liquid, a deformation of the analyte zone occurs because its frontal part moves at a relatively high velocity due to a low retention factor in the sample solvent while the rear part of the analyte zone is more retained in the carrier liquid and hence moves at a lower velocity. The influence of this solvent strength effect on the separation of analytes is studied here theoretically using a mass balance model describing the spatio-temporal evolution of the eluent, the sample solvent and the analyte. The viscosity of the sample solvent and carrier fluid is supposed to be the same (i.e. no viscous fingering effects are taken into account). A linear isotherm adsorption with a retention factor depending upon the local concentration of the liquid phase is considered. The governing equations are numerically solved by using a Fourier spectral method and parametric studies are performed to analyze the effect of various governing parameters on the dispersion and skewness of the analyte zone. The distortion of this zone is found to depend strongly on the difference in eluting strength between the mobile phase and the sample solvent as well as on the sample volume.

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“…Therefore, to increase the sensitivity when using the miniaturized technique, some approaches have been proposed to overcome the mentioned drawbacks, allowing analysts to applying nano-LC to real samples analysis. Among the different solutions proposed, the use of (1) sensitive and specific detectors, e.g., MS, (2) UV-detector cells with extended path length, (3) on-column focusing (Mills et al, 1997;Cappiello et al, 2002;Rocco et al, 2013b;Buonasera et al, 2009), and (4) pre-and/or 2D-column preconcentration are currently employed (Vissers et al, 1997).…”

Section: Improving Sensitivity In Nano-liquid Chromatographymentioning

confidence: 99%

Theory and Practice of UHPLC and UHPLC–MS

Guillarme

Veuthey

2017

Handbook of Advanced Chromatography /Mass Spectrometry Techniques

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Assessment of injection volume limits when using on-column focusing with microbore liquid chromatography

Cited by 91 publications

References 23 publications

Development of a high performance liquid chromatography/mass spectroscopy method for the determination of strychnine concentrations in insects used to assess potential risks to insectivores

Development of a high performance liquid chromatography/mass spectroscopy method for the determination of strychnine concentrations in insects used to assess potential risks to insectivores

Influence of a strong sample solvent on analyte dispersion in chromatographic columns

Theory and Practice of UHPLC and UHPLC–MS

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