Assessment of intracellular calcium levels in cryopreserved bovine sperm by flow cytometry

Collin, Simon; Bailey, Janice L.

doi:10.1016/s0093-691x(99)91900-3

Cited by 8 publications

(6 citation statements)

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“…Although several studies have reported high[Ca 2þ ]i in cryopreserved sperm compared with fresh sperm(Collin and Bailey 1999;Kadirvel et al 2009), the present study revealed a different picture. The percentage of live spermatozoa with high [Ca 2þ ]i levels was 53.8% for fresh semen, decreasing to 44.5% immediately after thawing (P , 0.05).…”

contrasting

confidence: 84%

“…Given that an increase in the concentration of calcium in sperm cells has been demonstrated during capacitation in several mammalian species, including swine , it could be speculated that such changes may also occur during cryocapacitation. In support of this theory, it has been shown that the levels of [Ca 2þ ]i were higher in cryopreserved than in fresh bovine spermatozoa (Collin and Bailey 1999).…”

Section: Introductionmentioning

confidence: 80%

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Quantification of kinetic changes in protein tyrosine phosphorylation and cytosolic Ca2+ concentration in boar spermatozoa during cryopreservation

Kumaresan

Siqueira

Hossain

et al. 2012

Reprod. Fertil. Dev.

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Protein tyrosine phosphorylation in sperm is associated with capacitation in several mammalian species. Although tyrosine phosphorylated proteins have been demonstrated in cryopreserved sperm, indicating capacitation-like changes during cryopreservation, these changes have not yet been quantified objectively. We monitored tyrosine phosphorylation, intracellular calcium and sperm kinematics throughout the cryopreservation process, and studied the relationships among them in boar spermatozoa. Sperm kinetics changed significantly during cryopreservation: curvilinear velocity, average path velocity and straight line velocity all decreased significantly (P < 0.05). While the percentage of sperm with high intracellular calcium declined (P < 0.05), global phosphorylation increased significantly (P < 0.01). Specifically, cooling to 5 °C induced phosphorylation in the spermatozoa. After cooling, a 32-kDa protein not observed in fresh semen appeared and was consistently present throughout the cryopreservation process. While the level of expression of this phosphoprotein decreased after addition of the second extender, frozen-thawed spermatozoa showed an increased expression. The proportion of sperm cells with phosphorylation in the acrosomal area also increased significantly (P < 0.05) during cryopreservation, indicating that phosphorylation might be associated with capacitation-like changes. These results provide the first quantitative evidence of dynamic changes in the subpopulation of boar spermatozoa undergoing tyrosine phosphorylation during cryopreservation.

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confidence: 84%

Section: Introductionmentioning

confidence: 80%

Quantification of kinetic changes in protein tyrosine phosphorylation and cytosolic Ca2+ concentration in boar spermatozoa during cryopreservation

Kumaresan

Siqueira

Hossain

et al. 2012

Reprod. Fertil. Dev.

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“…If the disk is geometrically thick (a "torus"), the exponent is smaller, F inc being proportional roughly to r −2 . This means that the density decreases rapidly with increasing radius, as about r −2 , as expected if the disk is in a state of marginal instability (Collin & Huré, 1999). On the other hand, for smaller radii, the emissivity varies slowly with r, which is compatible with the disk irradiated by the central source of continuum if the density is larger than 10 9 cm −3 .…”

Section: Modeling Of the Hβ Broad Componentsmentioning

confidence: 55%

Intermediate Resolution Hβ Spectroscopy and Photometric Monitoring of 3C 390.3. I. Further Evidence of a Nuclear Accretion Disk

Shapovalova¹,

Буренков²,

Spiridonova³

et al. 2002

International Astronomical Union Colloquium

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We have monitored the AGN 3C 390.3 between 1995 and 2000. A historical B-Band light curve dating back to 1966, shows a large increase in brightness during 1970 -1971, followed by a gradual decrease down to a minimum in 1982. During the 1995-2000 lapse the broad Hβ emission and the continuum flux varied by a factor of ≈ 3. Two large amplitude outbursts, of different duration, in continuum and Hβ light were observed ie.: in October 1994 a brighter flare that lasted ≈1000 days and in July 1997 another one that lasted ≈ 700 days were detected. The response time lag of the emission lines relative to flux changes of the continuum, has been found to vary with time ie. during 1995-1997 a lag of about 100 days is evident, while during 1998-1999 a double valued lag of ≈ 100 days and ≈ 35 days is present in our data. The flux in the Hβ wings and line core vary simultaneously, a behavior indicative of predominantly circular motions in the BLR. Important changes of the Hβ emission profiles were detected: at times, we found profiles with prominent asymmetric wings, as those normaly seen in Sy1s, while at other times, we observe profiles with weak almost symmetrical wings, similar to those of Sy1.8s. We further dismiss the hypothesis that the double peaked Hβ profiles in this object originate in a massive binary BH. Instead, we found that the radial velocity difference between the red and blue bumps is anticorrelated with the light curves of Hβ and continuum radiation. This implies that the zone that contributes with most of the energy to the emitted line, changes in radius within the disk. The velocity difference increases, corresponding to smaller radii, as the continuum flux decreases. When the continuum flux increases the hump velocity difference decreases. These transient phenomena are expected to result from the variable accretion rate close to the central source. The optical continuum and the Hβ flux variations might be related to changes in X-ray emission modulated by a variable accretion rate, changing the surface temperature of the disk, as a result of a variable X-ray irradiation ( Ulrich, 2000). Theoretical Hβ profiles were computed for an accretion disk, the observed profiles are best reproduced by an inclined disk (25 • ) whose region of maximum emission is located roughly at 200 Rg. The mass of the black hole in 3C 390.3, estimated from the reverberation analysis is Mrev ≈ 2.1 × 10 9 M ⊙ , 5 times larger than previous estimates (Wandel et al.1999).

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“…Several studies have reported higher [Ca 2+ ] i , capacitation, and acrosome reaction rate in freeze‐thawed sperm compared with fresh sperm . The researchers concluded that these changes in the capacitation‐like state were detrimental to fertility and sperm were rendered unstable and had a short lifespan.…”

Section: Discussionmentioning

confidence: 99%

Proteomic characteristics of human sperm cryopreservation

Wang

et al. 2014

Proteomics

125

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Human sperm cryopreservation in assisted reproductive technology is the only proven method that enables infertile men to father their own children. However, freezing and thawing reduces spermatozoon motility, viability, and fertilizing ability. An association between dysfunctional spermatozoa due to cryoinjury and protein changes has not been established. We investigated through proteomic analysis the differential protein characteristics between freeze-thawed and fresh sperm samples obtained from nine normozoospermic donors. Twenty-seven proteins differed in abundance between the two groups, and results were verified for four proteins via Western blot and immunofluorescent staining. These proteins are putatively involved in sperm motility, viability, acrosomal integrity, ATP and isocitrate content, mitochondrial membrane potential, capacitation, acrosome reaction, and intracellular calcium concentration. These marked differences suggest that dysfunctional spermatozoon after cryopreservation may be due to protein degradation and protein phosphorylation.

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Assessment of intracellular calcium levels in cryopreserved bovine sperm by flow cytometry

Cited by 8 publications

References 0 publications

Quantification of kinetic changes in protein tyrosine phosphorylation and cytosolic Ca2+ concentration in boar spermatozoa during cryopreservation

Quantification of kinetic changes in protein tyrosine phosphorylation and cytosolic Ca2+ concentration in boar spermatozoa during cryopreservation

Intermediate Resolution Hβ Spectroscopy and Photometric Monitoring of 3C 390.3. I. Further Evidence of a Nuclear Accretion Disk

Proteomic characteristics of human sperm cryopreservation

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