Attaining the objective of healthy lives and wellbeing Sustainable Development Goal 3 is hinged on effective health communication. This study investigates the information source usage, knowledge, attitude and practices of 200 dwellers of Lagos, Nigeria towards HIV/HBV co-infection. In this cross-sectional study, the simple random sampling method was employed in selecting 200 participants in the most populated local government in Lagos state, Nigeria. Questionnaire instrument was created, to elicit responses on four major areas: uses of information sources, knowledge, attitude, health practice towards HIV/HBV co-infection. Descriptive statistical data in percentages and cross-tabulations were employed. The study indicated that 60.4% of the respondents source for information on HIV/HBV co-infection on television. Furthermore, while 60% noted the co-infection is caused by a virus 48.5% declared that the fear of death will be their main concern if they were diagnosed with the co-infection. The result further reveals that only 26.8% of the sampled respondents have been tested for the co-infection in Lagos State. The study concludes that there is need for the creation of preventive information campaigns for awareness in order to mitigate the rising cases of HIV/HBV co-infection as well as influence persons towards healthy practices. The insight from this study would further provide a focal point of direction to government as well as non-governmental organisations working on HIV/HBV co-infection in Nigeria.