Assessment of Lemon Balm (Melissa officinalisL.) Hydrogels: Quality and Bioactivity in Skin Cells

Abstract

The aim of the study was to design gels with lemon balm extract, assess their quality, and investigate the effect of rosmarinic acid on skin cells in normal conditions and under oxidative stress. Methods. The quantities of rosmarinic acid (RA) released from gels were evaluated by applying the HPLC technique. HaCaT cell viability was assessed by using the MTT method. ROS generation was measured using DCFH-DA dye. The results showed that the gelling material affected the release of RA content from gels. Lower and slower RA content release was determined in carbomer-based gels. After 6 hours of biopharmaceutical research in vitro, at least 4% of RA was released from the gel. The results of the biological studies on HaCaT cells demonstrated that, in the oxidative stress conditions, RA reduced intracellular ROS amounts to 28%; 0.25–0.5 mg/mL of RA increased cell viability by 10–24% and protected cells from the damage caused by H2O2. Conclusions. According to research results, it is appropriate to use a carbomer as the main gelling material, and its concentration should not exceed 1.0%. RA, depending on the concentration, reduces the amount of intracellular ROS and enhances cell viability in human keratinocytes in oxidative stress conditions.

Formation and Investigation of Electrospun PLA Materials with Propolis Extracts and Silver Nanoparticles for Biomedical Applications

“…The cytotoxicity of microfiber materials was evaluated to assess their biological compatibility. Cell viability was assessed by MTT method [20]. The cells were seeded onto 96-well plates at a density of 20,000 cells/well and incubated for 24 h. After incubation, cells were treated with different formulations: propolis containing electrospun microfibers and microfibers without propolis.…”

Testing of resveratrol microemulsion photostability and protective effect against UV induced oxidative stress

“…Cell viability was assessed by the MTT method (11). The cells were seeded into 96-well plates at a density of 20,000 cells/well and incubated for 24 h. After incubation, cells were treated with different sample solutions: a) ME without added resveratrol (ME-control), b) ME-RES, and c) solution of resveratrol in DMSO (RES-control).…”

The Effect of Melissa Officinalis Syrup on Patients With Mild to Moderate Psoriasis: a Randomized, Double-blind Placebo-controlled Clinical Trial

“…Psoriasis is a disease with in ammation origin [27]. A number of previous studies indicated anti-oxidant, anti-in ammatory, and immunomodulatory activities of Melissa o cinalis, damask rose, and fennel [24,28,29]. These herbs are a rich source of phenolic compounds such as avonoids, cumarin, tannin, caffeic acid, caffeic acid, and rosmarinic acid [30][31][32].…”