Assessment of microbiologically influenced corrosion in oilfield water handling systems using molecular microbiology methods

Senthilmurugan, Balasubramanian; Radhakrishnan, Jayaprakash S.; Poulsen, Morten; Tang, Lone; AlSaber, Shouq

doi:10.1016/j.upstre.2021.100041

Cited by 13 publications

(8 citation statements)

References 8 publications

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“…Total Cell density isolation was gauged through serial dilution in sterile broth and plating on Anaerobic Blood Agar. The most probable number assay estimated SRB density ( Senthilmurugan et al, 2021 ). Biomass of the entire microbial community was determined using the Bradford protein assay ( Bradford, 1976 ).…”

Section: Methodsmentioning

confidence: 99%

Harnessing sulfate-reducing bacteria with plants growing to revitalize metal-tainted coal mine soils in Midwest China: metal sequestration performance, ecological networking interaction, and functional enzymatic prediction

Yang,

Wu,

Liu

et al. 2023

Front. Microbiol.

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Heavy metal contamination from coal mining calls for advanced bioremediation, i.e., using sulfate-reducing bacteria (SRB) technology. Yet, the interaction of SRB with native soil microbiota during metal sequestration, especially in the presence of plants, remains ambiguous. In this study, we assessed the metal sequestration capabilities, ecological network interactions, and enzymatic functions in soils treated with a predominant SRB consortium, mainly Desulfovibrio (14 OTUs, 42.15%) and Desulfobulbus (7 OTUs, 42.27%), alongside Acacia dealbata (AD) and Pisum sativum (PS) plants. The SRB consortium notably enhanced the immobilization of metals such as Zn, Cu, As, and Pb in soil, with the conversion of metals to residual forms rising from 23.47 to 75.98%. Plant inclusion introduced variability, potentially due to changes in root exudates under metal stress. While AD flourished, PS demonstrated significant enhancement in conjunction with SRB, despite initial challenges. Comprehensive microbial analyses revealed the pivotal role of SRB in influencing microbial networking, underpinning critical ecological links. This interplay between plants and SRB not only enhanced microbial diversity but also enriched soil nutrients. Further, enzymatic assessments, highlighting enzymes like NADH:ubiquinone reductase and non-specific serine/threonine protein kinase, reinforced contribution of SRB to energy metabolism and environmental resilience of the entire soil microbial community. Overall, this research underscores the potential of SRB-driven bioremediation in revitalizing soils affected by coal mining.

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Section: Methodsmentioning

confidence: 99%

Harnessing sulfate-reducing bacteria with plants growing to revitalize metal-tainted coal mine soils in Midwest China: metal sequestration performance, ecological networking interaction, and functional enzymatic prediction

Yang,

Wu,

Liu

et al. 2023

Front. Microbiol.

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“…In the praxis, monitoring many microbial groups is unfeasible and therefore it is important to establish a handful of microbes that can be routinely monitored, in a sense, acting as proxy for the overall microbial threat. Although one can argue that in the last decades such a role has been fulfilled by cultivable SRB and APB (Sowards et al, 2014;Okoro and Amund, 2015;Permeh et al, 2017;Senthilmurugan et al, 2021), the recent technological advances have brought to light the need to rethink both the microorganisms being monitored and the way they are monitored (Mukherjee et al, 2017;Koerdt, 2021;McDaniel et al, 2021;Pilloni et al, 2022).…”

Section: Mic Biodiversity As Source For Biasesmentioning

confidence: 99%

“…In terms of absolute quantification by qPCR, the use of the same nucleic acids extraction protocols and 16S primer pairs used for sequencing is recommended for consistency and bias reduction. A more robust characterization can include the use of functional marker genes for additional quantification of discrete physiological groups such as sulfate reducers (apsA, dsrA genes) and methanogenic archaea (mcrA) (Ben-Dov et al, 2007;Schippers and Blazejak, 2011;McKay et al, 2017;Senthilmurugan et al, 2021). The integration of relative and absolute abundances of the different taxonomic levels and physiological groups allows to identify and pinpoint the most relevant populations targeted for mitigation plans as well as for routine monitoring.…”

Section: Assembling a Roadmap For Mic Field Monitoringmentioning

confidence: 99%

Microbiologically influenced corrosion: The gap in the field

Puentes-Cala¹,

Tapia-Perdomo²,

Espinosa-Valbuena³

et al. 2022

Front. Environ. Sci.

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Microorganisms have evolved to inhabit virtually all environments on the planet, from oceanic hot-seeps to pipelines transporting crude and refined hydrocarbons. Often microbial colonization of man-made structures results in the reduction of their service life requiring preemptive or corrective human intervention. Microbiologically Influenced Corrosion (MIC) is caused by a set of intricate bioelectrochemical interactions between a diverse group of microorganisms and metallic surfaces. The complexity of MIC microbiomes and their mechanisms as well as the logistics constraints of industrial facilities are factors to consider when choosing suitable analytical methods for MIC monitoring. These generally reflect only a partial view of the phenomenon and in consequence, might lead to ineffective mitigation measures. This paper acknowledges the discrepancies between the fieldwork for MIC monitoring and the currently available technological advancements. It also highlights the most pressing issues that operators have in the field in light of the diversity of the microbial key players present in corrosive microbiomes. Finally, it compiles and outlines a strategy for the integration of novel molecular approaches aiming for a practical and accurate assessment of the microbial threat.

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“…qPCR (quantitative polymerase chain reaction) untuk memantau gen tertentu yang diminati. Amplifikasi dan sekuensing gen 16S rRNA untuk mengidentifikasi jenis mikrobiota yang ada dalam sampel lingkungan [49].…”

Section: Metode Investigasi Dan Deteksi Micunclassified

“…Sistem assessment menggunakan metode kupon untuk mengukur laju korosi, mikrobiologi molekuler qPCR dan NGS (next generation sequencing) dapat mendeteksi kelompok mikroorganisme bakteri pereduksi sulfat Desulfovibrio dalam jumlah besar. Hasilnya telah memberikan gambaran rinci tentang status mikroba dari berbagai sistem dan lebih dapat dipercaya daripada metode MPN (most probable number) konvensional [49].…”

Section: Metode Investigasi Dan Deteksi Micunclassified

Biokorosi Dan Teknologi Pencegahannya Di Industri Minyak Dan Gas

Royani¹,

Hanafi²,

Julistiono³

et al. 2022

metal.

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Biokorosi atau Microbiologically Influenced Corrosion (MIC) adalah salah satu bentuk korosi lokal yang dihasilkan dari mikroorganisme dan aktivitasnya yang mempunyai dampak negatif pada fasilitas di industri minyak dan gas. Keberadaan mikroorganisme dan aktivitasnya yang kompleks memainkan peranan penting dalam pembentukan biofilm dan produk korosi yang menyebabkan kerusakan peralatan. Meskipun beberapa teknik mitigasi dan pencegahan dalam usaha pengendalian mikroorganisme telah dilakukan, tinjauan tentang mitigasi korosi yang dipengaruhi mikroorganisme masih terbatas. Dalam tinjauan ini, dibahas jenis mikroorganisme terkait biokorosi, pembentukan biofilm, teknik deteksi dan evaluasi mikroorganisme, teknologi pencegahan dan pengendalian mikroorganisme di industri minyak dan gas. Perkembangan teknologi terkini pengendalian mikroorganisme di industri lainnya juga turut dibahas.

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Assessment of microbiologically influenced corrosion in oilfield water handling systems using molecular microbiology methods

Cited by 13 publications

References 8 publications

Harnessing sulfate-reducing bacteria with plants growing to revitalize metal-tainted coal mine soils in Midwest China: metal sequestration performance, ecological networking interaction, and functional enzymatic prediction

Harnessing sulfate-reducing bacteria with plants growing to revitalize metal-tainted coal mine soils in Midwest China: metal sequestration performance, ecological networking interaction, and functional enzymatic prediction

Microbiologically influenced corrosion: The gap in the field

Biokorosi Dan Teknologi Pencegahannya Di Industri Minyak Dan Gas

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