Assessment of micronuclei and sister chromatid exchange frequency in the petroleum industry workers in province of Vojvodina, Republic of Serbia

Mrdjanović, Jasminka; Šolajić, Slavica; Dimitrijević, Sladjana; Ðan, Igor; Nikolić, Ivan; Jurišić, Vladimir

doi:10.1016/j.fct.2014.03.041

Cited by 16 publications

(15 citation statements)

References 32 publications

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“…However, some benzene metabolites are also present in coffee (hydroquinone and catechol) and many foods (phenol). Age, smoking status, and length of occupational exposure must also be carefully considered as additional factors in the evaluation of DNA damage (Tunsaringkarn et al 2011;Mrdjanović et al 2014;Mansi et al 2012;Lovreglio et al 2014). Bukvic et al (1998) observed that the occurrence of SCE was significantly associated with both age and smoking.…”

Section: Discussionmentioning

confidence: 98%

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Association between occupational exposure to benzene and chromosomal alterations in lymphocytes of Brazilian petrochemical workers removed from exposure

Gonçalves

Melo

Rêgo

2016

Environ Monit Assess

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We aimed to investigate the association between chronic exposure to benzene and genotoxicity in the lymphocytes of workers removed from exposure. The study included 20 workers with hematological disorders who had previously worked in the petrochemical industry of Salvador, Bahia, Brazil; 16 workers without occupational exposure to benzene served as the control group. Chromosomal analysis was performed on lymphocytes from peripheral blood, to assess chromosomal breaks and gaps and to identify aneuploidy. The Kruskal-Wallis test was used to compare the mean values between two groups, and Student's t test for comparison of two independent means. The frequency of gaps was statistically higher in and the exposed group than in the controls (2.13 ± 2.86 vs. 0.97 ± 1.27, p = 0.001). The frequency of chromosomal breaks was significantly higher among cases (0.21 ± 0.58) than among controls (0.12 ± 0.4) (p = 0.0002). An association was observed between chromosomal gaps and breaks and occupational exposure to benzene. Our study showed that even when removed from exposure for several years, workers still demonstrated genotoxic damage. Studies are still needed to clarify the long-term genotoxic potential of benzene after removal from exposure.

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Section: Discussionmentioning

confidence: 98%

“…Chemical mutagenicity is a complex process that depends on individual genetic susceptibility, as well as on the duration and severity of exposure, and can be influenced by other modifying factors (Mrdjanović et al 2014).…”

Section: Introductionmentioning

confidence: 99%

Association between occupational exposure to benzene and chromosomal alterations in lymphocytes of Brazilian petrochemical workers removed from exposure

Gonçalves

Melo

Rêgo

2016

Environ Monit Assess

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“…Mutagenic tests have been applied to study the chromosome fragility (or stability) on cells exposed both in vivo and in vitro to mutagens or chemicals, suspected to have mutagenic activity (Mrdjanovic et al, 2014;Lovreglio et al, 2014). In particular, the cytogenetic tests we used in the present study have been applied in both human and animal lymphocytes, often revealing a higher chromosome fragility, compared to control groups (in vivo exposure) or control unexposed cells (in vitro exposure) (Rubes et al, 1997;Lioi et al, 1998;Picco et al, 2004;Iannuzzi et al, 2004;Perucatti et al, 2006;Santoro et al, 2008;Bonassi et al, 2008;Di Meo et al, 2011;Genualdo et al, 2012;Siviková and Dianovsky, 2006;Sivikova et al, 2013;Georgieva et al, 2013;Genualdo et al, 2015;Perucatti et al, 2015;Iannuzzi et al, 2016).…”

Section: Discussionmentioning

confidence: 99%

Cytogenetic tests reveal no toxicity in lymphocytes of rabbit ( Oryctolagus cuniculus, 2n=44) feed in presence of verbascoside and/or lycopene

Perucatti

Genualdo

Pauciullo

et al. 2018

Food and Chemical Toxicology

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Phenylpropanoid glycosides (PPG), like other phenolic compounds, are a powerful antioxidants and the Verbascoside (VB) is one of the most active of them. A previous study, by using in vitro exposure of blood human lymphocytes to Verbascoside, reported a significant increasings of chromosome fragility compared to control. In the present study, four homogeneous groups of rabbits were used to test in vivo the VB and/or Lycopene (LP) by feeding the animals without VB and LP (control), in presence of VB or/and LP for 80 days. Lymphocyte cell cultures were performed in three different times: 0, 40 and 80 days of the experiment and the cytogenetic tests that we used [CA-test (Chromosome Abnormalities in terms of chromosome and chromatid breaks) and Sister Chromatid Exchange (SCE-test)] have revealed no mutagenic effects on chromosomes. Indeed, mean values/cell of CA and SCE decreased during the experiment with some difference among and within groups, with significant decreasing value only for some group. The study shows clear evidence that diets rich in Verbascoside (and/or Lycopene) do not originate any mutagenic activity, resulting no cytotoxic for the animals and, suggesting a possible their use in both animal and human diets.

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“…Cytogenetic tests are very useful to check the presence of chromosome damage due to the mutagens present in the environment (Lovreglio et al 2014;Mrdjanovic et al 2014), in the food chain and more specifically in livestock animals, which are below humans in the food chain (Iannuzzi et al 2004;Perucatti et al 2006;Di Meo et al 2011). In addition, high frequencies of chromatid breaks have been found in blood cells from a high percentage of cancer patients (Bryant et al 2004).…”

Section: Animals and Dioxin Analysesmentioning

confidence: 99%

Chromosome instability in lymphocytes of Friesian cows naturally exposed to dioxins being raised close to a metallurgic factory area in southern Italy

et al. 2016

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Dioxins (PCDDs, PCDFs and DL-PCBs) are a large family of congeners that are considered highly toxic and are reported to be teratogenic, mutagenic, carcinogenic, immunotoxic and hepatotoxic, also affecting the nervous and reproductive systems. Farm animals are particularly exposed to these chemicals when they are fed with grass produced close to polluted areas such as those located in vicinity of metallurgic factories. Cytogenetic tests can be very useful to check genetic damage occurring to domestic animal cells exposed to these chemicals. Fiftytwo randomly selected Italian Friesian cows (Bos taurus, 2n = 60) from two farms located in the vicinity of and (as a control) far from the a metallurgic industrial area underwent cytogenetic investigations to ascertain possible differences in their chromosome fragility. One farm was under legal sequestration due to the presence in the milk mass of higher mean values of dioxins (24.78 ± 3.19 pg g −1 of fat as sum of PCDD + PCDF + DL-PCBs as WHO-TEQ (World Health Organization-Toxic Equivalent Quantity), with DL-PCBs being the main chemical component) than those permitted (5.5 pg g −1 of fat as WHO-TEQ). Cytogenetic analyses, performed by using both the chromosome abnormality (CA) test (chromosome and chromatid breaks) and sister chromatid exchange (SCE) test, revealed a significantly (p < 0.01) higher chromosome fragility in cells of exposed cows (26 cows) compared to those of the control (23 cows).

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Assessment of micronuclei and sister chromatid exchange frequency in the petroleum industry workers in province of Vojvodina, Republic of Serbia

Cited by 16 publications

References 32 publications

Association between occupational exposure to benzene and chromosomal alterations in lymphocytes of Brazilian petrochemical workers removed from exposure

Association between occupational exposure to benzene and chromosomal alterations in lymphocytes of Brazilian petrochemical workers removed from exposure

Cytogenetic tests reveal no toxicity in lymphocytes of rabbit ( Oryctolagus cuniculus, 2n=44) feed in presence of verbascoside and/or lycopene

Chromosome instability in lymphocytes of Friesian cows naturally exposed to dioxins being raised close to a metallurgic factory area in southern Italy

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