Assessment of micronuclei frequency in individuals with a habit of tobacco by means of exfoliated oral buccal cells

Dosi, Tanvi; Gupta, Dhaman; Hazari, Alka; Rajput, Rajan; Chauhan, Prabhav; Rajapuri, Anushri S

doi:10.4103/2231-0762.189745

Cited by 10 publications

(6 citation statements)

References 23 publications

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“…These findings are biologically coherent and are compatible with the literature, in which some authors also verified in their respective studies a direct relationship between the frequency of MN and tobacco consumption. 21,22 Like Namala et al, in the present study we verified that cells of the control group occasionally presented MN, but MN frequency was statistically higher in the smoker group, confirming that tobacco consumption increases MN frequency. 5 This represents the cytogenetic damage caused by smoking as being quite significant.…”

Section: Discussionsupporting

confidence: 88%

Association between DNA ploidy and micronucleus frequency in chronic smokers and impact of smoking cessation

et al. 2021

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Tobacco use may initiate the process of oral carcinogenesis with clinically undetectable changes. Smoking cessation may prevent its progression. The objective of this study was to evaluate the association between DNA ploidy and micronucleus (MN) frequency in chronic smokers. Three groups were evaluated: Smoker Group, Former Smoker Group and Control Group. Exfoliative cytology was performed on the lateral border of the tongue and mouth floor. MN and DNA ploidy analyses were performed, as well as the correlation between the variables. The data showed a difference between the groups for the total MN (p = 0.0227), and the Smoker group had the highest mean (4.22 ± 4.12). The three groups did not differ statistically from each other on ploidy evaluation (p-value > 0.05). There was also an association between aneuploidy and increased MN frequency in the Former Smoker group (p = 0.0036). In conclusion, these results point out that there is a relationship between the frequency of MN and aneuploidy in former smokers. Moreover, smoking cessation, even for a short period of time, may promote the decrease of MN frequency caused by tobacco use.

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Section: Discussionsupporting

confidence: 88%

Association between DNA ploidy and micronucleus frequency in chronic smokers and impact of smoking cessation

et al. 2021

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“…The duration of addiction of the chewing habit in the present study of 50 individuals was in average of 1-20 years, which is in accordance with the study done by Jyoti et al 13 In present study, the tobacco chewers and betel quid chewers have shown a greater number of MN as compared to the healthy control group. This finding relates to that of Sellappa et al, 14 Palaskar and Jindal, 15 Joshi et al, 16 Dosi et al, 17 and Sharma et al 4 We also observed variations in the frequency of micronucleated cells in the control group, which may be probably due to the different food habits within the population group as individuals are exposed to various chemicals in their daily life, which was the reason for the variable levels of micronucleated cells. 13 On the other hand, Bansal et al 18 and Ozkul et al 19 in their work showed a higher level of the mean number of MN in smokeless tobacco users as compared with smokers, thus indicating higher carcinogenic potential of smokeless tobacco.…”

Section: As Followssupporting

confidence: 86%

“…There is a three-fold higher MN rate per cell in the group of tobacco chewers and tobacco chewers with lesion patients than in the control group, and a number of studies have shown a gradual increase in MN frequency from normal to precancerous to cancerous lesions as done by Palaskar and Jindal, 15 Jyoti et al, 13 Caplash et al, 11 and Dosi et al 17 Cytobrushes appear to be most effective for collecting large numbers of cells, and the high expense makes them less feasible for routine purposes. So, we preferred the use of a wooden spatula.…”

Section: As Followsmentioning

confidence: 91%

Cytogenetic Analysis of Micronuclei in Tobacco Chewers: A Study in North Indian Population

Vaid

Bhargava

Bansal

et al. 2019

The Journal of Contemporary Dental Practice

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Aim: Oral cancer ranks in top three of all cancers in India and accounts for over 30% of all cancers reported in the country. The present study aims at an in vivo cytogenetic analysis to assess the applicability of the micronucleus test in diagnosing early detection of dysplastic changes. Materials and methods: Exfoliated scrape smears were collected from 75 patients suffering from premalignant lesions. The wet-fixed smears were stained by adopting Papanicolaou's staining protocol. The analysis of variance (ANOVA) test was used to analyze the data statistically. Results: The results of the above study give a fair assessment of the amount of nuclear alterations seen in individuals exposed to genotoxic agents, such as tobacco, and also to a certain extent, an insight into the expected biological behavior of the lesions present in such individuals. Detection of micronuclei and their assay is an upcoming research domain in the field of cancer detection and therapeutics. These miniature nuclear offshoots if properly identified can turn out to be important biomarkers with huge potential for screening and predicting patients with oral precancers and also can act as risk assessors in patient's ongoing treatment for invasive cancers. Conclusion:The frequency of micronucleated cells has been observed to be in increasing order with the increase in the age-groups and from control to precancerous cases significantly in both sexes.

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“…These structures have similar appearance to those structures which can be observed in the cellular nuclei during of interphase. 4,5 Therefore, in the present study we aimed to evaluate the genotoxic effects of tobacco chewing by means of micronucleus assay in exfoliated cells of buccal mucosa.…”

Section: Introductionmentioning

confidence: 99%

Assessment of Micronuclei Frequency in Individuals with a Habit of Tobacco Chewing by Means of Exfoliated Oral Buccal Cells

Parhar¹,

Mahajan²

2020

IHRJ

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INTRODUCTION: Majority of cancers are diagnosed at an advanced stage resulting in poor prognosis and survival rates among patients. Hence early diagnosis of oral cancers seems to be the need of the hour. Analysis of exfoliated buccal cell micronuclei (MN) is a sensitive method of monitoring genetic damage. AIM: The present study has been carried out with an objective to evaluate the genotoxic effects of tobacco chewing by means of micronucleus assay in exfoliated cells of buccal mucosa.MATERIALS AND METHOD: This cross sectional study was carried out in Department of Oral Pathology, Swami Devi Dyal Hospital And Dental College, Golpura, Barwala, Panchkula. The study population comprised of a total of 50 subjects, divided into five groups: Group 1comprising of 10 age and sex matched healthy subjects without any habits as controls, Group 2 comprising of 10 subjects with a history of chewing tobacco. Group 3 comprising of 10 subjects with a history of chewing tobacco and cigarette smoking, Group 4 comprising of 10 subjects with a history of chewing tobacco and drinking and Group 5 comprising of 10 subjects with a history of chewing tobacco, smoking and drinking. Oral exfoliated cells were obtained from buccal mucosa of the subjects, slides were prepared from each subject stained with stain respectively.RESULTS: The mean numbers of micronuclei in group 1 were 7.86±6.7, Group 2 were 63.37±10.01, Group 3 were 65.49±12.32, Group 4 were 68.22±11.11 and Group 5 were 69.43±10.71. On comparison we observed that the difference in Mean micronuclei frequency among all the 5 study groups came to be statistically also highly significant (p<0.0001*)CONCLUSION: Micronuclei assay is an effective tool that reflects severity of disease. Even though tobacco induced cancers are preventable, banning the use of tobacco has not been possible for social and political reasons.

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Assessment of micronuclei frequency in individuals with a habit of tobacco by means of exfoliated oral buccal cells

Cited by 10 publications

References 23 publications

Association between DNA ploidy and micronucleus frequency in chronic smokers and impact of smoking cessation

Association between DNA ploidy and micronucleus frequency in chronic smokers and impact of smoking cessation

Cytogenetic Analysis of Micronuclei in Tobacco Chewers: A Study in North Indian Population

Assessment of Micronuclei Frequency in Individuals with a Habit of Tobacco Chewing by Means of Exfoliated Oral Buccal Cells

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