Background: Although the domain of psychiatric nursing is a demanding profession with certain aspects that are more stressful than others, strategies intended for enhancing emotional regulation may help psychiatric nurses deal with the stress of their jobs. The analysis of how psychiatric nurses use emotion regulation strategies to regulate their emotions during times of stress and how this affects their wellbeing at work is critical. This study aimed to evaluate job stress, emotion regulation, and their relation to workplace wellbeing among psychiatric nurses. Methods: A descriptive correlational research design was employed. Setting: This study was conducted at Beni-Suef Psychiatric Mental Health Hospital. Subjects: At the time of the study's data collection, all 79 psychiatric nurses who were available in the above-mentioned setting. Tools: Four tools were used to gather data. Tool 1: Personal and job characteristics data Sheet. Tool II: Psychiatric Nurse Job Stress Scale (PNJSS). Tool III: Emotion Regulation Questionnaire (ERQ). Tool VI: Eudaimonic workplace well-being scale (EWWS). Results: A strong positive association was observed between workplace well-being and emotion regulation at p value <0.01**,while a strong negative correlation was detected between job stress and workplace well-being at p value 0.001**. Also, there was a high negative correlation between job stress and emotion regulation at p value 0.002**. Conclusion: These results demonstrated how experiencing high levels of job stress was substantially correlated with both poor emotional regulation and workplace well-being. The study recommended: Implementing emotion regulation-based intervention programs to lessen working stress and enhance the wellbeing of psychiatric nurses.