Assessment of Multiplate platelet aggregometry using citrate, heparin or hirudin in Rhesus macaques

Dugan, Greg; O’Donnell, Lisa; Hanbury, David B.; Cline, J. Mark; Caudell, David L.

doi:10.3109/09537104.2014.988694

Cited by 18 publications

(12 citation statements)

References 17 publications

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“…However, some differences in aggregation between hirudinated and heparinated PRP could not be explained so readily. It has been reported that heparin is capable of inducing the micro-aggregate formation in PRP, which are potentiating further platelet aggregation as well as false-positive LTA results 39 . Furthermore, it has been demonstrated that heparin is a positive mediator of platelet integrin function 40 and thus, in heparinized PRP platelet aggregation might be additionally potentiated.…”

Section: Discussionmentioning

confidence: 99%

Quantitative dynamics of reversible platelet aggregation: mathematical modelling and experiments

Filkova

Martyanov

Dasgupta

et al. 2019

Sci Rep

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Although reversible platelet aggregation observed in response to ADP stimulation in the presence of calcium is a well-known phenomenon, its mechanisms are not entirely clear. To study them, we developed a simple kinetic mass-action-law-based mathematical model to use it in combination with experiments. Light transmission platelet aggregometry (LTA) induced by ADP was performed for platelet-rich plasma or washed platelets using both conventional light transmission and aggregate size monitoring method based on optical density fluctuations. Parameter values of the model were determined by means of parameter estimation techniques implemented in COPASI software. The mathematical model was able to describe reversible platelet aggregation LTA curves without assuming changes in platelet aggregation parameters over time, but with the assumption that platelet can enter the aggregate only once. In the model, the mean size of platelet aggregates correlated with the solution transparency. This corresponded with flow cytometry analysis and with optical density fluctuations data on aggregate size. The predicted values of model parameters correlated with ADP concentration used in experiments. These data suggest that, at the start of the aggregation, when platelet integrins switch “on”, large unstable platelet aggregates are rapidly formed, which leads to an increase in light transmission. However, upon fragmentation of these aggregates, the probability of the post-aggregate platelets’ attachment to each other decreases preventing new aggregation and resulting in the reversible aggregation phenomenon.

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Section: Discussionmentioning

confidence: 99%

Quantitative dynamics of reversible platelet aggregation: mathematical modelling and experiments

Filkova

Martyanov

Dasgupta

et al. 2019

Sci Rep

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“…The clinical applications of platelet reactivity testing has put a focus on development of novel strategies for platelet reactivity assessment [ 14 ]. But with the development of new and improved hemostatic assessment methods, the issue of the correct pre-analytical conditions, such as which anticoagulant and concentration to use, has been receiving increased attention from researchers [ 7 – 10 , 15 ]. Thus, the aim of our study was to clarify some of the issues regarding anticoagulant use in LTA.…”

Section: Discussionmentioning

confidence: 99%

“…It was also reported that both ADP and collagen caused weaker platelet impedance responses in citrated whole blood samples compared to hirudinized samples [ 8 ]. The inferiority of sodium citrate as an anticoagulant in impedance whole-blood aggregometry has thus been well documented [ 7 – 10 ]. Nevertheless, conclusive evidence with an extensive range of the Clinical and Laboratory Standards Institute (CLSI) Platelet Function Testing by Aggregometry Approved Guideline recommended agonists [ 11 ], using both sodium citrate and hirudin as anticoagulants in LTA, is still lacking.…”

Section: Introductionmentioning

confidence: 99%

Comparison of Commercially Available Blood Collection Tubes Containing Sodium Citrate and Hirudin in Platelet Aggregation Testing

Rensburg

Merwe

2017

Med Sci Monit Basic Res

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BackgroundPlatelet reactivity assessment is an important tool in both the causal determination of bleeding diathesis as well as in the evaluation of the efficacy of anti-platelet therapy in patients at risk of thrombosis. Sodium citrate is the most widely used anticoagulant for hemostasis investigations. However, some doubt exists over the suitability of sodium citrate in platelet function testing. Hirudin has been suggested as a superior replacement. Nevertheless, only 1 study compared citrated and hirudin treated samples with light transmission aggregometry. Therefore, limited evidence exists to conclusively prove the supremacy of hirudin over sodium citrate in light transmission aggregometry. The aim of our study was to compare citrated and hirudin treated samples, collected in commercially available blood collection tubes, using the 5 most common agonists, with light transmission aggregometry.Material/MethodsBlood was obtained from 20 healthy volunteers. Platelet counts were performed on platelet-rich plasma. Light transmission aggregometry was performed within 4 h of sample collection using ADP, collagen, arachidonic acid, epinephrine, and ristocetin as agonists.ResultsPlatelet counts for the respective anticoagulants did not differ significantly. ADP-induced aggregation was comparable between the samples. However, among all the agonists, hirudin-treated platelets had significantly weaker aggregatory responses.ConclusionsCommercially available sodium citrate should remain the anticoagulant of choice for routine platelet function testing in our setting. However, the time limitation associated with the use of sodium citrate in platelet function testing remains a concern. Thus, alternative anticoagulants should still be explored.

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“…Previous results have shown that platelet aggregation in heparin anti-coagulated blood is higher than in sodium citrate anti-coagulated blood using different blood samples. 12,13 In sodium citrate anti-coagulated blood, Ca 2+ in the blood, which is necessary for platelet activation and aggregation, chelates with sodium citrate. However, Ca 2+ levels do not decrease in heparin anti-coagulated blood, as calcium chelation does not occur.…”

Section: Discussionmentioning

confidence: 99%

Quantitative investigation of platelet aggregation under high shear force for anti-platelet aggregation in vitro tests

et al. 2021

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Blood pumps are often used for hemofiltration in patients with renal failure. To design effective centrifugal blood pumps for hemofiltration, it is important to suppress clogging caused by platelet aggregation. However, the optimal conditions for conducting anti-platelet aggregation tests in vitro have not yet been established. This study aimed to quantify the effect of the shear loading value and shear loading time on platelet aggregation and determine the optimal conditions for anti-platelet aggregation testing in vitro. To quantitatively evaluate platelet aggregation in terms of the negative logarithm-platelet aggregation threshold index (NL-PATI), which reflects the propensity of residual platelets to aggregate after shear loading, the following parameters were examined: blood collection method (collected from porcine vein using a syringe or collected from a slaughterhouse), type of anticoagulant (sodium citrate or heparin), shear rate, and shear time. The results showed that platelet aggregation in porcine blood increased under a high shear load applied at shear rates of approximately 20,000 s−1 or higher for 30 s. Platelet aggregation propensity was 2–3 times higher in heparin-anticoagulated blood than in sodium citrate-anticoagulated blood. Moreover, platelet aggregation was 1.5–2 times more in blood collected from the slaughterhouse than in syringe-collected blood. Testing with an integrated shear time of 30 s or less in relation to the total blood volume may be effective for conducting in vitro circulation experiments using hemofiltration blood pumps. The conditions established in this study may be useful for hemocompatibility testing of cardiovascular devices based on NL-PATI.

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Assessment of Multiplate platelet aggregometry using citrate, heparin or hirudin in Rhesus macaques

Cited by 18 publications

References 17 publications

Quantitative dynamics of reversible platelet aggregation: mathematical modelling and experiments

Quantitative dynamics of reversible platelet aggregation: mathematical modelling and experiments

Comparison of Commercially Available Blood Collection Tubes Containing Sodium Citrate and Hirudin in Platelet Aggregation Testing

Quantitative investigation of platelet aggregation under high shear force for anti-platelet aggregation in vitro tests

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