Assessment of NaCl-Induced Stress in Tomato (Lycopersicon esculentum L.)

Abstract

Comparative study about the salt-induced oxidative stress and lipid peroxidation has been realised in primary root tissues for Tomato (Lycopersicon esculantum L.) in order to evaluate their responses to salt stress. Salinity impacts in terms of root growth, H2O2 generation, lipid peroxidation and membrane destabilisation were more pronounced in roots. Salt treatment in form of NaCl was given to the roots of the tomato plants in hydroponics culture. Root length was measured by centimetre scale, H2O2 and lipid peroxidation was confirmed by spectrophotometer. Absorbance for H2O2 estimation was recorded at 480 nm whereas for Lipid peroxidation was done at 600nm. When the tomato plants were treated with different concentrations of NaCl, it was observed that as the concentration of NaCl was increasing, there was decreased root growth resulting in reduced root length and proportionate increase in the amount of H2O2 production level with increase in the concentrations of NaCl treatment upto 300mM Concentration and Significant increase in Lipid peroxidation was observed with the increase in NaCl concentrations upto 500mM Concentration. Comparative response may be helpful in developing a better understanding of tolerance mechanisms to salt stress in Tomato.

A Simple and Effective Bioassay Method Suitable to Comparative In Vitro Study of Tomato Salt Tolerance at Early Development Stages

“…However, a number of studies have shown clear correlation between tomato salt tolerance under in In vitro evaluation of tomato genotypes for salt tolerance has undoubted advantages (high productivity, as well as stability and reproducibility of the obtained experimental data due to the maintenance of constant controlled conditions) in comparison with openfield system and pot experiments (Figure 9). The authors cultivated seeds [21][22][23], shoot apexes [48], shoot apical meristem [49], and callus [50][51][52][53] as an explant source under salinity treatments in vitro. The disadvantage of seeds as an explant source is high heterogeneity, as well as greatly differ in the uniformity of germination capacity and germination energy.…”

“…Many studies carried out to testing of tomato salinity tolerance during germination of non-sterile seeds under stress treatment [21][22][23] or aseptically seed germinated on culture media, containing various NaCl or Na 2 SO 4 concentrations [24][25][26][27]. However, even high-quality seeds greatly differ in the uniformity of germination capacity and germination energy.…”