Assessment of nuclear transfer techniques to prevent the transmission of heritable mitochondrial disorders without compromising embryonic development competence in mice

Single Ca2+ transients vs oscillatory Ca2+ signaling for assisted oocyte activation: limitations and benefits

“…Experiments with MF1 mice (light gray), are normalized to blastocyst formation rate obtained by Saunders et al (2002), which was 87%. Experiments with B6D2F1 mice (dark gray) are normalized to blastocyst formation rate obtained by Neupane et al (2014), which was 91%. Only blastocyst formation rate reported after the use of TPEN was obtained in same mouse strain (B6D2F1) but in a different lab.…”

Patients with a high proportion of immature and meiotically resistant oocytes experience defective nuclear oocyte maturation patterns and impaired pregnancy outcomes

“…Several studies have demonstrated that GV nuclear or cytoplasmic transfer represent potential treatment techniques to alleviate oocyte meiosis arrest, shown in the maturation resistant mouse model (Hoffmann et al, 2012) and for human oocyte maturation defects (Cohen et al, 1997;Zhang et al, 1999). Spindle transfer has also been successfully applied for the treatment of mitochondrial disease (Neupane et al, 2014). However, considering the compromised IP3R1 distribution and impaired spindle-chromosome configuration observed in both human and mouse IVM and maturation resistant oocytes from our Study groups, the application of these methodologies requires further validation.…”

Reconstruction of mammalian oocytes by germinal vesicle transfer: A systematic review

“…Micromanipulation procedures were carried out on a warm stage placed on a micromanipulator system including an inverted light microscope, micro-pipette injector/puller and holder, gas pressure regulator, glass capillary tubing, micrometre syringe, manipulation chambers, and anti-vibration table (4,(7)(8)(9)(10)(11)(12). The system could be supplemented with a specimen incubator, charge-coupled device camera, shutter controller, epi-fluorescence, digital image processing software, and computer (7)(8)(9)(10)(13)(14)(15)(16).…”

“…Nuclear transfer (NT) is one of the most widely used techniques that seemingly be helpful to improve the ooplasm quality and increase the mitochondria activity through removing the chromosomes from oocyte and transferred into enucleated donated one (3)(4)(5). Germinal vesicle transfer (GVT) is possible to enucleate GV stage oocytes before they mature to the MII stage and use them as recipients for NT.…”