Assessment of parental contributions to fast- and slow-growing progenies in the sea bream Sparus aurata L. using a new multiplex PCR

Borrell, Yaisel J.; Gallego, Víctor; García-Fernández, Carmen; Mazzeo, I.; Pérez, Luz; Asturiano, Juan F.; Carleos, Carlos; Vázquez, Esther; Sánchez, José A.; Blanco, Gloria

doi:10.1016/j.aquaculture.2011.01.028

Cited by 33 publications

(45 citation statements)

References 45 publications

(51 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The power statistics of the forensic parameters was high, allowing parentage studies of C. macropomum. These results are similar to those with other multiplex genotyping systems of microsatellites in aquaculture, such as the ones described by Borrell et al (2011).…”

Section: Discussionsupporting

confidence: 80%

Short Communication Multiplex PCR panel of microsatellite markers for the tambaqui, Colossoma macropomum, developed as a tool for use in conservation and broodstock management

Hamoy

Santos²

2012

Genet. Mol. Res.

View full text Add to dashboard Cite

ABSTRACT. The tambaqui, Colossoma macropomum, native to Brazil, is widely used in aquaculture systems. We developed a multiplex PCR panel for this species, comprising 12 microsatellite loci. This panel was used to genotype 73 specimens collected from Juruti, a city in the Brazilian Amazon. The mean number of alleles per locus was 8.8, the mean observed heterozygosity was 0.76, and the combined power of discrimination and the combined power of exclusion were 0.99999999999999993 and 0.999991762, respectively. We observed no significant deviation from Hardy-Weinberg equilibrium in this population. All amplified alleles were clearly typed, and easily interpretable results were obtained. This method will be useful for paternity analysis, population genetics and conservation studies, as well as for selective breeding programs for C. macropomum.

show abstract

Section: Discussionsupporting

confidence: 80%

Short Communication Multiplex PCR panel of microsatellite markers for the tambaqui, Colossoma macropomum, developed as a tool for use in conservation and broodstock management

Hamoy

Santos²

2012

Genet. Mol. Res.

View full text Add to dashboard Cite

show abstract

“…High polymorphism was observed in the present study signifying its powerful identification capability for these parentage data. Similar results were reported by various authors in other aquaculture species (MacAvoy et al 2008;Borrell et al 2011;Ma et al 2013). Six microsatellite loci in this study exhibited significant deviation from the Hardy-Weinberg equilibrium .…”

Section: Discussionsupporting

confidence: 93%

A molecular tool for parentage analysis in Indian major carp, Labeo rohita (Hamilton, 1822)

et al. 2016

View full text Add to dashboard Cite

Determination of genealogical relationship between broodstock and progeny is important to estimate selection response in aquaculture breeding programs. Currently, the application of microsatellite markers for parentage determination is gaining popularity in aquaculture. In the present study, 17 simple sequence repeat markers were evaluated for parentage assignment on rohu. The allele frequencies, exclusion probabilities and polymorphic information contents (PIC) were calculated using Cervus. The polymorphic information content values showed that most of the microsatellite markers were highly informative (PIC > 0.7). Based on the exclusion power, eight microsatellite markers were chosen for parentage assignment. The robustness of this suite was assessed in five full-sib and four halfsib families using two contrasting methods, a pair-wise likelihood comparison approach in Cervus and a full-pedigree likelihood method in the COLONY program. Using real data set, the correct matching rate was more than 98%, comparable to the simulated study (99.9%) in the case of Cervus 3.0 and 100% in the case of COLONY. The result of the present study implies that this set of microsatellites would be an effective tool for parentage and sibship identification, testing performance of families and estimating genetic parameters in the ongoing selective breeding program of rohu.

show abstract

“…To assay SNPs in SaTf, genomic DNA from eye tissue of twenty seabream, previously identified as unrelated through microsatellite analyses [38], was isolated using ZymoBeadÔ Genomic DNA kit (ZYMO RESEARCH) according to the manufacturer's protocol. The complete SaTf genomic sequence characterized in this study was amplified and sequenced (as described above) for each of the selected individuals.…”

Section: Snp Analysismentioning

confidence: 99%

Characterization of the gilthead seabream (Sparus aurata L.) transferrin gene: Genomic structure, constitutive expression and SNP variation

García-Fernández

Sánchez

Blanco

2011

Fish & Shellfish Immunology

Self Cite

View full text Add to dashboard Cite

Assessment of parental contributions to fast- and slow-growing progenies in the sea bream Sparus aurata L. using a new multiplex PCR

Cited by 33 publications

References 45 publications

Short Communication Multiplex PCR panel of microsatellite markers for the tambaqui, Colossoma macropomum, developed as a tool for use in conservation and broodstock management

Short Communication Multiplex PCR panel of microsatellite markers for the tambaqui, Colossoma macropomum, developed as a tool for use in conservation and broodstock management

A molecular tool for parentage analysis in Indian major carp, Labeo rohita (Hamilton, 1822)

Characterization of the gilthead seabream (Sparus aurata L.) transferrin gene: Genomic structure, constitutive expression and SNP variation

Contact Info

Product

Resources

About