Assessment of Phage-Mediated Inhibition and Removal of Multidrug-Resistant Pseudomonas aeruginosa Biofilm on Medical Implants

Amankwah, Stephen; Adisu, Mekonen; Gorems, Kasahun; Abdella, Kedir; Kassa, Tesfaye

doi:10.2147/idr.s367460

Cited by 17 publications

(8 citation statements)

References 36 publications

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“…However, some studies have shown that the studied phages retain their initial titer of viral particles only at pH 7.0–8.0 [ 66 ] and that the virions of the studied bacteriophages do not withstand extreme pH levels (2.0 and 12.0) [ 67 ]. Thus, in studies of the biological properties of lytic myophage MJ1 against multiresistant strains of E.coli, it was found that this bacteriophage had maximum stability at pH 7 and was also relatively stable at pH 5 and 9.…”

Section: Discussionmentioning

confidence: 99%

Isolation and Characterization of Jumbo Coliphage vB_EcoM_Lh1B as a Promising Therapeutic Agent against Chicken Colibacillosis

Alexyuk¹,

Bogoyavlenskiy²,

Alexyuk³

et al. 2023

Microorganisms

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Colibacillosis in chickens can cause the death of young stock, decrease weight gain and lead to significant economic losses. Currently, antibiotic therapy is the main method of treatment of infected animals, but unchecked use of antibiotics has led to widespread antibiotic resistance among microorganisms. Therefore, it is necessary to develop alternative methods of treating bacterial infections that are fully consistent with the One Health concept and introduce them into practice. Phage therapy meets the specified requirements perfectly. This study describes the isolation and characterization of the lytic jumbo phage vB_EcoM_Lh1B and evaluates its potential use in controlling antibiotic-resistant E. coli infection in poultry. The complete phage genome is 240,200 bp long. Open reading frame (ORF) prediction shows that the phage genome does not contain genes encoding antibiotic resistance and lysogeny factors. Based on phylogenetic and electron microscopic analysis, vB_EcoM_Lh1B belongs to the group of myoviruses of the Seoulvirus genus of the Caudoviricetes class. The bacteriophage has good resistance to a wide range of pH and temperatures and has the ability to suppress 19 out of 30 studied pathogenic E. coli strains. The biological and lytic properties of the isolated vB_EcoM_Lh1B phage make it a promising target of further study as a therapeutic agent against E. coli infections in poultry.

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Section: Discussionmentioning

confidence: 99%

Isolation and Characterization of Jumbo Coliphage vB_EcoM_Lh1B as a Promising Therapeutic Agent against Chicken Colibacillosis

Alexyuk¹,

Bogoyavlenskiy²,

Alexyuk³

et al. 2023

Microorganisms

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“…The host range of propagated phages were tested for phage activity following the standard spot test procedure described earlier 18 against E. coli strains and other non- E. coli bacteria. One hundred μL of each of the host cells at log phase were added to 5 mL of sterile molten soft agar (0.8%) and poured over a previously prepared Nutrient Agar plate surface.…”

Section: Methodsmentioning

confidence: 99%

Isolation and Phenotypic Characterization of Virulent Bacteriophages Against Multidrug-Resistant Escherichia coli and Its Phage-Resistant Variant from Sewage Sources

Fikadu,

Amankwah,

Alemu

et al. 2024

IDR

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Purpose The use of lytic bacteriophages for the control or elimination of pathogenic multidrug-resistant (MDR) bacteria is the promising alternative. However, the emergence of resistant bacterial variants after phage application may challenge its therapeutic benefit. In this study, we aimed to isolate candidate phages from sewage samples against two MDR Escherichia coli as well as their phage-resistant variant. Methods MDR E. coli isolates (n = 10) obtained from Jimma Medical Center that had been properly identified and stored were used to isolate bacteriophages. Two lytic coliphages were isolated from hospital sewage samples following standard protocols. Upon single phage infection, phage-resistant variant quickly evolved serving as a new host for the isolation of a third lytic phage. This virulent phage’s lytic activity against both its host and the wild host was investigated. The host infectivity of the various cocktails was assessed, and each phage’s biological properties were studied. Results Out of the first round of phage isolation process, two lytic phages were identified as VBO- E. coli 4307 and VBW- E. coli 4194. When exposed to VBO- E. coli 4307, the wild-type E. coli 4307 developed resistant variants. A third phage (VBA- E. coli 4307R) was isolated specific to this resistant variant ( E. coli 4307R) under optimum condition. For VBO- E. coli 4307, VBW- E. coli 4194, and VBA- E. coli 4307R, the plaque assays generated under comparable conditions were 2.13 × 10 10 PFU mL −1 , 9.17 × 10 12 PFU mL −1 , and 3.3 × 10 10 PFU mL −1 , respectively. These phages have nearly identical stability and lytic ability but differ greatly in their host ranges for VBA- E. coli 4307R. Conclusion While the wild-type MDR pathogen could easily evolve resistance when exposed to a single phage infection by VBO- E. coli 4307, it is still possible to isolate a novel bacteriophage from environmental samples that is effective against the phage-resistant variants. This indicates that it is possible to manage the effects of phage resistance pathogens even if they are MDR.

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“…In Table 2 are summarized some studies found in the literature regarding bio-inspired antimicrobial coatings [ 115 , 116 ].…”

Section: Modern Technological Advances In Antimicrobial Coatings For ...mentioning

confidence: 99%

Antimicrobial Solutions for Endotracheal Tubes in Prevention of Ventilator-Associated Pneumonia

Marcut,

Manescu (Paltanea),

Antoniac

et al. 2023

Materials

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Ventilator-associated pneumonia is one of the most frequently encountered hospital infections and is an essential issue in the healthcare field. It is usually linked to a high mortality rate and prolonged hospitalization time. There is a lack of treatment, so alternative solutions must be continuously sought. The endotracheal tube is an indwelling device that is a significant culprit for ventilator-associated pneumonia because its surface can be colonized by different types of pathogens, which generate a multispecies biofilm. In the paper, we discuss the definition of ventilator-associated pneumonia, the economic burdens, and its outcomes. Then, we present the latest technological solutions for endotracheal tube surfaces, such as active antimicrobial coatings, passive coatings, and combinatorial methods, with examples from the literature. We end our analysis by identifying the gaps existing in the present research and investigating future possibilities that can decrease ventilator-associated pneumonia cases and improve patient comfort during treatment.

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Assessment of Phage-Mediated Inhibition and Removal of Multidrug-Resistant Pseudomonas aeruginosa Biofilm on Medical Implants

Cited by 17 publications

References 36 publications

Isolation and Characterization of Jumbo Coliphage vB_EcoM_Lh1B as a Promising Therapeutic Agent against Chicken Colibacillosis

Isolation and Characterization of Jumbo Coliphage vB_EcoM_Lh1B as a Promising Therapeutic Agent against Chicken Colibacillosis

Isolation and Phenotypic Characterization of Virulent Bacteriophages Against Multidrug-Resistant Escherichia coli and Its Phage-Resistant Variant from Sewage Sources

Antimicrobial Solutions for Endotracheal Tubes in Prevention of Ventilator-Associated Pneumonia

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