Assessment of Plane Dipslide Quantitation of Bacteriuria

Abstract

The reliability of the Uricult dipslide in the quantitative estimation of bacteriuria has been tested on 2,596 urines from out-patients and in-patients of various types. The dipslide results at 15–20^°C and at 37°C correspond almost exactly with pour-plate controls in 340 urines. False-positive and false-negative results were negligible. The dipslide results correspond well, but not so completely, with those from 1,000 urines tested by the less accurate standard-loop technique. Dipping by ward sisters and auxiliary nurses was assessed for accuracy in 1,168 specimens. A number of other studies are reported. On the basis of our results the dipslide system is now being used routinely in our hospital group. It is strongly recommended that a dipslide culture is obtained by the medical practitioner and despatched to the laboratory before antimicrobial therapy is started on a patient with suspected urinary tract infection. Dipslide culture at 15–20°C or 37°C is considered to be as accurate as pour-plate technique and more accurate and reliable than the conventional standard-loop method at 37 °C.

Urinary Tract Infection in Diabetic Patients

“…Quantitative urine culture was performed using a dip-slide method (Urotube, Becton Dickinson) and urine was also streaked on MacConkey agar. This dip-slide method has been shown to correlate well with conventional methods [21,22]. After incubation at 37°C for 24 h, the microorganisms were identified by standard biochemical tests.…”

A Double‐blind Trial of Half‐strength Polybactrin Soluble GU Bladder Irrigation in Cystoscopy

“…In the present trial a more detailed search for infection has been made in an attempt to obtain an accurate incidence of post-instrumentation infection, with 4 serial urine samples being examined over a 2-week period after the cystoscopy (see Patients and Methods). Since 3 of the 4 post-cystoscopy urine samples were to be given after the patient's discharge from hospital, dip slides were used as a screening test since it has been shown that they are accurate in detecting urinary tract infections (Guttman and Naylor, 1967;McAllister et al, 1973). This technique was backed up by MSU analysis where infection was suspected from the dip slide results.…”

“…Bacterial growth occurring on the dip slides was described as confluent, semi-confluent or moderate. A confluent or semi-confluent growth of bacteria on the dip slides has been found to correlate with significant bacteriuria (Guttman and Naylor, 1967;McAllister et al, 1973).…”

Current Microbiological and Clinical Aspects of Urinary Tract Infections

“…This dip–slide method has been shown to correlate well with conventional methods [1, 2]. After incubation at 37°C for 24 h the microorganisms were identified by standard biochemical tests.…”