Assessment of polyphenolic compounds, in vitro antioxidant and anti-inflammation properties of Securidaca longepedunculata root barks

Abstract

The present study was carried out to evaluate the antioxidant activities of the root bark extract of Securidaca longepedunculata. This plant material is commonly used in folk medicine in several parts in the world. The bark extracts of S. longepedunculata were evaluated for their total phenols, flavonoids, anthocyanins, tannins content and total antioxidant capacity. The compounds were identified and quantified both by RP-HPLC and UV spectrophotometer; the antioxidant capacity was assessed by ABTS and DPPH tests and expressed as IC 50 . The total phenolic compounds determinate was 9.86 mg gallic acid equivalents/g dw, the total flavonoid contents was 5.85 mg catechin equivalents/g dw, the total anthocyanin contents was 0.032 mg cyanidin-3-glycosyl equivalents/g dw and the condensed tannins content were 1.03 mg catechin equivalents/g dw. The major compound identified using RP-HPLC was quercetin (0.98 mg/ml). The IC 50 value reached 5.5 mg/ml, revealing that the root barks of S. longepedunculata have a very high antioxidant and antiinflammation properties.

Securidaca longipedunculata Fresen (Polygalaceae): A review of its ethnomedicinal uses, phytochemistry, pharmacological properties and toxicology

“…The aqueous methanol extract (50%) of the root bark exhibited an IC 50 of 1.351 and 9.48 µg/ml against ABTS and DPPH respectively (Muanda et al, 2010). Although the extraction methods of the leaf and root bark were slightly different, these results may well suggest that the root bark extract quenches DPPH much better than the leaf extract.…”

“…The water and aqueous methanol extracts from the root yielded a variety of compounds in varying amounts, including gallic acid, chlorogenic acid, caffeic acid, epicatechic acid, rutin, p-coumaric acid, cinnamic acid, apigenin, quercetin glucosyl and quercetin dihydrate (Muanda et al, 2010). Four highly oxygenated xanthones, muchimangins A-D, with a diphenylmethyl substituent have also been isolated from the root as minor constituents (Dibwe et al, 2012).…”

“…The 50% aqueous methanol extract showed good anti-inflammatory activity in a dose dependent manner by exhibiting reduction of NO production in macrophages stimulated with LPS/IFN-gamma yielding 51.3% inhibition at a concentration of 150 µl (Muanda et al, 2010). The methanol extracts, petroleum ether and methanol fractions obtained from solvent extraction of the root bark were also investigated for anti-inflammatory properties using topical edema of the mouse ear model (Okoli et al, 2005).…”

Diet-related inflammation and oesophageal cancer by histological type: a nationwide case–control study in Sweden

“…In these studies, low-fat diet [22], fruit [25], tomatoes [23,26], nuts [27], whole grains [21,28], fish [29], and, especially, nutrients from foods rich in phytochemicals or antioxidants (e.g. carotene, lycopene, vitamin C, flavonoids [30][31][32]) have been found to have anti-inflammatory properties. In contrast, high-fat foods (e.g.…”

Acute and Sub-Acute Oral Toxicity Studies of Aqueous Extract of Securidaca Longepedunculata Fresen (Polygalaceae) Root Barks in Rodents.

“…Indeed, pharmacological studies on S longepedunculata showed some anti-inflammatory, antiulcer, antianemia and antiplasmodial properties of this species extracts (Ojewole et al, 2008). Furthermore, phytochemical investigations of several parts of the plant revealed presence of saponins (Ndamitso et al, 2013;Stevenson et al, 2009), flavonoids (Auwal et al, 2012;Muanda et al, 2010), alkaloids (Wrobel et al, 1996), steroids (Meli et al, 2007), sucrose derivatives (De Tommasi et al, 1993), phenolic acids (Muanda et al, 2010) and volatile oils (Nebié et al, 2004;Jayasekara et al, 2005). Although a lot of medicinal plants are widely used and assumed to be safe, they could potentially be toxic (Nasri and Shirzad, 2013).…”