Assessment of Retinal and Choroidal Blood Flow Changes Using Laser Doppler Flowmetry in Rats

Hétu, Simon; Pouliot, Mylène; Cordahi, Ghassan; Couture, Réjean; Vaucher, Elvire

doi:10.3109/02713683.2012.723296

Cited by 9 publications

(9 citation statements)

References 51 publications

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“…The vessel diameter and blood flow changes we observed in response to hyperoxia and hypercapnia were similar to published values (Kiss et al, 2002; Sato et al, 2003; Gilmore et al, 2005; Venkataraman et al, 2006; Sehi et al, 2012; Werkmeister et al, 2014), and the time course of the blood flow changes seen were comparable to those previously observed in the rat (Hétu et al, 2013). The Hagen-Poiseuille relation predicts that under constant perfusion pressure, blood flow should vary as the fourth power of vessel diameter.…”

Section: Discussionsupporting

confidence: 90%

Measurement of Retinal Blood Flow Using Fluorescently Labeled Red Blood Cells

Kornfield

Newman

2015

eneuro

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Section: Discussionsupporting

confidence: 90%

Measurement of Retinal Blood Flow Using Fluorescently Labeled Red Blood Cells

Kornfield

Newman

2015

eneuro

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“…However, 23 induced one or more BRAO. Of these, 13 used lasers,7–18 five used embolization,19–23 one used heat coagulation by the use of a probe,24 and four used a probe or an occluder 24–27…”

Section: Resultsmentioning

confidence: 99%

“…Apart from one study using serotonin, vasoconstriction was induced by administration of endothelin-1, which is a potent vasoconstrictor produced by vascular endothelial cells 39. Generally, endothelin-1 was either injected in the posterior vitreous body over the optic disc, which causes constriction of all retinal vessels, including both veins and arteries,16,38,39,40 or it was injected in the subconjunctival space in the posterior part of the eyeball causing constriction of the CRA 41. It is not evident if this last method causes constriction of other vessels too.…”

Section: Resultsmentioning

confidence: 99%

Animal Models Used to Simulate Retinal Artery Occlusion: A Comprehensive Review

Vestergaard

Cehofski

Honoré

et al. 2019

Trans. Vis. Sci. Tech.

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Purpose To present an overview of animal models of retinal artery occlusion (RAO). Methods Through a systematic literature search in PubMed and Embase, papers describing methods of inducing RAO in animal models were included. The identified methodologic approaches were presented in a narrative synthesis and compared with RAO in humans. Results In total, 83 papers reporting on 88 experiments were included. Six different species were used with rodents and monkeys being the most common, and a minority were performed using cats, dogs, rabbits, or pigs. The anatomy of pigs and monkeys resemble that of humans most closely. The two most frequently used methods were laser-induced occlusion or ligation of the arteries. Other methods included raised intraocular pressure, arterial clamping, administration of vasoconstricting agents, the use of an occluder, embolization, and endovascular approaches to induce occlusion. In general, occlusions lasted for only 30 to 90 minutes, often followed by reperfusion. Conclusions Although a broad range of methods have previously been used, they all have limitations. Preferably, the methods should imitate the human disease as closely as possible and avoid damaging other structures. Therefore, monkeys followed by pigs are to be preferred and ligation or clamping may be a suitable model in larger animals as there is a potential to isolate and occlude the retinal artery only. Being less invasive, laser-induced occlusion is another suitable approach. Translational Relevance This review aims at assisting researchers in deciding on the most ideal experimental setting, and thereby increase the translational value to human disease.

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“…This device should be considered as a new tool to assess retinal perfusion along with the other devices recently reported: LDF to assess global ocular perfusion [ 8 ], an invasive method to acquire perfusion information [ 16 ] and the promising but less affordable OCT-OMAG technique [ 11 ].…”

Section: Discussionmentioning

confidence: 99%

“…Researchers have access to different rodent models of systemic (diabetes, hypertension) and ocular (glaucoma, diabetic retinopathy, nonarteritic ischemic optic neuropathy, hereditary optic nerve and retinal) diseases. For rats, noninvasive techniques of ocular blood perfusion measurement have previously been developed, including LDF [ 8 ], laser speckle [ 9 ], optical coherence tomography (OCT) [ 10 ], optical microangiography (OMAG) [ 11 ], magnetic resonance imaging (MRI) [ 12 ], the scanning laser ophthalmoscope-particle tracking method [ 13 ] and scanning LDF [ 14 ]. Many of these techniques require specific and expensive equipment for animal studies (OCT, MRI, OMAG) and highly qualified operators.…”

Section: Introductionmentioning

confidence: 99%

Compact Laser Doppler Flowmeter (LDF) Fundus Camera for the Assessment of Retinal Blood Perfusion in Small Animals

et al. 2015

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PurposeNoninvasive techniques for ocular blood perfusion assessment are of crucial importance for exploring microvascular alterations related to systemic and ocular diseases. However, few techniques adapted to rodents are available and most are invasive or not specifically focused on the optic nerve head (ONH), choroid or retinal circulation. Here we present the results obtained with a new rodent-adapted compact fundus camera based on laser Doppler flowmetry (LDF).MethodsA confocal miniature flowmeter was fixed to a specially designed 3D rotating mechanical arm and adjusted on a rodent stereotaxic table in order to accurately point the laser beam at the retinal region of interest. The linearity of the LDF measurements was assessed using a rotating Teflon wheel and a flow of microspheres in a glass capillary. In vivo reproducibility was assessed in Wistar rats with repeated measurements (inter-session and inter-day) of retinal arteries and ONH blood velocity in six and ten rats, respectively. These parameters were also recorded during an acute intraocular pressure increase to 150 mmHg and after heart arrest (n = 5 rats).ResultsThe perfusion measurements showed perfect linearity between LDF velocity and Teflon wheel or microsphere speed. Intraclass correlation coefficients for retinal arteries and ONH velocity (0.82 and 0.86, respectively) indicated strong inter-session repeatability and stability. Inter-day reproducibility was good (0.79 and 0.7, respectively). Upon ocular blood flow cessation, the retinal artery velocity signal substantially decreased, whereas the ONH signal did not significantly vary, suggesting that it could mostly be attributed to tissue light scattering.ConclusionWe have demonstrated that, while not adapted for ONH blood perfusion assessment, this device allows pertinent, stable and repeatable measurements of retinal blood perfusion in rats.

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Assessment of Retinal and Choroidal Blood Flow Changes Using Laser Doppler Flowmetry in Rats

Cited by 9 publications

References 51 publications

Measurement of Retinal Blood Flow Using Fluorescently Labeled Red Blood Cells

Measurement of Retinal Blood Flow Using Fluorescently Labeled Red Blood Cells

Animal Models Used to Simulate Retinal Artery Occlusion: A Comprehensive Review

Compact Laser Doppler Flowmeter (LDF) Fundus Camera for the Assessment of Retinal Blood Perfusion in Small Animals

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