Assessment of salivary thioredoxin levels in oral lichen planus and oral squamous cell carcinoma

Abstract

Objective: Oral lichen planus (OLP) is a chronic mucocutaneous inflammatory disease, which is considered as a potentially malignant condition and could transformed into oral squamous cell carcinoma (OSCC). Squamous cell carcinmoma is the most common oral cancer. This study aimed to compare salivary thioredoxin levels as an antioxidant protein among patients with OSSC, OLP and healthy subjects.Materials and methods: Twenty-eight patients with OLP, 20 patients with OSCC and 40 healthy people enrolled in this observational study. Saliva samples were collected from all subjects and salivary thioredoxin levels were evaluated by Elisa test. The data were recorded in the check lists and analyzed using SPSS (ver.17).Results: Thioredoxin levels of healthy controls were insignificantly higher than OLP and SCC patients (p = 0.135). There was a statistically remarkable indirect relationship between thioredoxin levels and severity of the lesions determined by Thongprasom criteria among OLP patients. The thioredoxin concentration was significantly higher in the keratotic OLP. Among the OSCC patients, the highest levels of thioredoxin were found among patients aged more than 65 years. Salivary thioredoxin levels based on other variables were not significantly different between the studied groups. Conclusion:In this study, there was not any significant difference between salivary thioredoxin levels in the OLP and OSCC patients, though it was unremarkable higher in the healthy group compared to the patients; therefore, the role of thioredoxin in the cellular oxidation-reduction status could be suggested; however, further studies are recommended.

Label-Free Proteomics of Oral Mucosa Tissue to Identify Potential Biomarkers That Can Flag Predilection of Precancerous Lesions to Oral Cell Carcinoma: A Preliminary Study

“…In our study, we found that levels of thioredoxin 1 and peroxiredoxin 1 were downregulated in cancer as compared to those of leukoplakia and erythroplakia, respectively. A lower level of salivary TXN has also been reported in patients with ulcerative SCC compared to those with leukoplakia and erythroplakia [ 37 ]. Reduced levels of thioredoxin and other antioxidants react with ROS contributing to the characteristic increased levels of oxidative stress in cancer cells [ 38 ].…”

CD8 + T-cell marker genes reveal different immune subtypes of oral lichen planus by integrating single-cell RNA-seq and bulk RNA-sequencing

“…OLP is considered an inflammatory disease of the oral cavity mediated by immunity. However, it has been shown that 1.63% of OLP evolves into OSCC within 7 years and is therefore classified as a potentially malignant disease by WHO [ 26 , 27 ].…”