Assessment of single nucleotide polymorphisms in genes residing on chromosomes 14 and 29 for association with carcass composition traits in Bos indicus cattle1,2

Abstract

Objective of this study was to assess the association of SNP in the diacylglycerol O-acyltransferase 1 (DGAT1), thyroglobulin (TG), and micromolar calcium-activated neutral protease (CAPN1) genes with carcass composition and meat quality traits in Bos indicus cattle. A population of Brahman calves (n = 479) was developed in central Florida from 1996 to 2000. Traits analyzed were ADG, hip height, slaughter weight, fat thickness, HCW, marbling score, LM area, estimated KPH fat, yield grade, retail yield, sensory panel tenderness score, carcass hump height, and cooked meat tenderness measured as Warner-Bratzler shear force at 7, 14, and 21 d postmortem. Single nucleotide polymorphisms previously reported in the TG and DGAT1 genes were used as markers on chromosome 14. Two previously reported and two new SNP in the CAPN1 gene were used as markers on chromosome 29. One SNP in CAPN1 was uninformative, and another one was associated with tenderness score (P < 0.05), suggesting the presence of variation affecting meat tenderness. All three informative SNP at the CAPN1 gene were associated with hump height (P < 0.02). The TG marker was associated with fat thickness and LMA (P < 0.05), but not with marbling score. No significant associations of the SNP in the DGAT1 gene were observed for any trait. Allele frequencies of the SNP in TG and CAPN1 were different in this Brahman population than in reported allele frequencies in Bos taurus populations. The results suggest that the use of molecular marker information developed in Bos taurus populations to Bos indicus populations may require development of appropriate additional markers.

Genetic polymorphisms related to meat traits in purebred and crossbred Nelore cattle

“…Higher GG frequency was prevalent in all but one group, the Rubia Gallega x Nelore, in which the AG genotype was most prominent. In contrast with the results presented by Casas et al (2005), which showed absence of the A allele in a B. indicus population of the Brahman breed, this study revealed a 21.9% frequency in Nelore B. indicus. Thus, for the fi rst time, it was possible to study the association between this polymorphism and traits of economical interest in B. indicus bovines.…”

“…According to Casas et al (2005), polymorphisms that segregate and associate with traits of interest in B. taurus may or may not segregate in B. indicus. Thus, it is necessary to develop and use other markers in candidate genes or chromosomal regions, to allow association studies in B. indicus.…”

Profiling of the yak skeletal muscle tissue gene expression and comparison with the domestic cattle by genome array

“…The possible reasons may be because yak and cattle belong to Poephagus and Bos, respectively, owing to the differences in genome between two bovine species, the gene regulation mode may change. Casas et al (2005) have demonstrated that the allele frequencies of some molecular marker locus in chromosomes 14 and 29 associated with carcass traits are different between the domestic cattle (Bos taurus) and zebu cattle (Bos indicus). SNPs in the TG gene were significantly associated with the marble scores in domestic cattle, but no association in zebu castles, suggesting that new suitable molecular markers need to be found in zebu populations.…”

A meta-analytic assessment of a Thyroglobulin marker for marbling in beef cattle

“…The data for Study 6 was first analysed in [4]. The excluded studies were described in [22] and [5]. All other studies were unpublished technical reports provided by Genetic Solutions Pty.…”

“…Casas et al [5] investigated the association between the TG5 marker and USDA marble score in a sample of 467 Brahman (Bos indicus) cattle. They found no association, but the sample included only 18 CT and 7 TT animals.…”