Assessment of sperm chromatin condensation and ploidy status using flow cytometry correlates to fertilization, embryo quality and pregnancy following in vitro fertilization

Lazaros, Leandros; Vartholomatos, George; Hatzi, Elissavet; Kaponis, Apostolos; Makrydimas, G.; Kalantaridou, Sophia Ν.; Sofikitis, Nikolaos; Stefos, Theodoros; Zikopoulos, Konstantinos; Georgiou, Ioannis

doi:10.1007/s10815-011-9611-z

Cited by 35 publications

(21 citation statements)

References 47 publications

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“…We noted also that the degree of sperm chromatin compaction was significantly correlated with the average number of sperm head abnormalities and the acrosome abnormalities. These results confirm earlier investigations [12, 19, 24, 28–30] that demonstrated significant correlations between sperm morphology and its nuclear condensation. Similarly, Kazerooni et al [17] showed a significantly higher percentage of AB-positive spermatozoa among men with teratozoospermia when compared with normozoospermic group (31.6% versus 14.1%; P < 0.001).…”

Section: Discussionsupporting

confidence: 93%

Assessment of Chromatin Maturity in Human Spermatozoa: Useful Aniline Blue Assay for Routine Diagnosis of Male Infertility

Sellami

Chakroun

Zarrouk

et al. 2013

Advances in Urology

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During spermatogenesis, sperm chromatin undergoes structural changes and results in a high condensation. This nuclear compaction would be useful as a predictor of sperm fertilization capacity and pregnancy outcome. We purpose to evaluate firstly the relationship among chromatin maturity assessed by aniline blue staining (AB) and the semen parameters in infertile men. Secondly, we analyzed whether the sperm gradient density centrifugation is effective to select mature spermatozoa. Fifty-one ejaculates were investigated by semen analysis and stained for chromatin condensation with AB to distinguish between unstained mature sperm and stained immature sperm. AB was applied also on 12 ejaculates which proceeded by density gradient centrifugation to compare the rates of immature sperm before and after selection. Neat semen were divided into two groups: G1 (n = 31): immature sperm <20% and G2 (n = 20): immature sperm ≥20%. No significant differences were detected in sperm concentration, motility, and normal morphology between G1 and G2. However, the rates of some morphology abnormalities were higher in G2: head abnormalities (P = 0.01) and microcephalic sperm (P = 0.02). We founded significant correlation between sperm immaturity and acrosome abnormalities (r = 0.292; P = 0.03). Sperm selection has significantly reduced the rates of immature sperm. A better understanding of chromatin structure and its impact on the sperm potential is needed to explore male infertility.

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Section: Discussionsupporting

confidence: 93%

Assessment of Chromatin Maturity in Human Spermatozoa: Useful Aniline Blue Assay for Routine Diagnosis of Male Infertility

Sellami

Chakroun

Zarrouk

et al. 2013

Advances in Urology

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“…Probably, the presence of high percentages of histone in morphologically normal spermatozoa can be a sign of defective genome silencing and faulty imprinting pattern. Studies on condensation anomalies correlating ART outcome revealed that protamine deficiency negatively affects both fertilization rate and pronuclear morphology (Nasr-Esfahani et al, 2006;Lazaros et al, 2011). In the present study, normal spermatozoa with condensation defects had a negative effect on fertilization rate in IVF; that was supported by another study where risk of fertilization failure was higher in the samples with increased percentages of abnormal spermatozoa and condensation defects (Esterhuizen et al, 2002).…”

Section: Discussionsupporting

confidence: 80%

“…With respect to sperm parameters, properly condensed sperm nucleus is essential to undergo decondensation for pronucleus formation during fertilization (Tosti & Fortunato, 2011). Declined rate of fertilization, embryo quality, and pregnancy outcome were also recorded in semen samples with aneuploid and immatured spermatozoa (Lazaros et al, 2011). Moreover, higher prevalence of abnormal chromatin condensation was marked in teratozoospermia cases Utsuno et al, 2013).…”

Section: Introductionmentioning

confidence: 99%

“…Studies revealed 30% oocytes fail to fertilize in normal condition that may be due to sperm abnormalities and/or defects in oocyte activation factors (Swain & Pool, 2008;Kahyaoglu et al, 2014). Declined rate of fertilization, embryo quality, and pregnancy outcome were also recorded in semen samples with aneuploid and immatured spermatozoa (Lazaros et al, 2011). Declined rate of fertilization, embryo quality, and pregnancy outcome were also recorded in semen samples with aneuploid and immatured spermatozoa (Lazaros et al, 2011).…”

Section: Introductionmentioning

confidence: 99%

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An illustration of human sperm morphology and their functional ability among different group of subfertile males

et al. 2018

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Condensed sperm chromatin is a prerequisite for natural fertilization. Some reports suggested the prevalence of chromatin condensation defects in teratozoospermia cases with head anomalies; conversely, earlier studies exemplified its occurrence in morphologically normal spermatozoa too. The aim of this study was to compare the condensation defects in correlation with head anomalies among different groups of subfertile males and its impact on the rate of fertilization in assisted reproduction procedures. Ultrastructure analysis of spermatozoa through scanning electron microscopy and atomic force microscopy could facilitate an in-depth evaluation of sperm morphology. Nuclear condensation defects (%) in spermatozoa were analyzed in 666 subjects, and its effect on the rate of fertilization was analyzed in 116 IVF and 90 intracytoplasmic sperm injection cases. There was no correlation of condensation defects with head anomalies (%). Student's t-test showed no significant changes in mean values of condensation defects in abnormal semen samples in comparison with the normal group. Condensation defects were observed in normal spermatozoa too, which was negatively associated with the rate of fertilization in IVF (p < 0.01), but intracytoplasmic sperm injection outcome remained unaffected. Ultrastructure study revealed sperm morphological features in height, amplitude, and three-dimensional views in atomic force microscopy images presenting surface topography, roughness property of head, and compact arrangement of mitochondria over axoneme with height profile at nanoscale. In pathological forms, surface roughness and nuclear thickness were marked higher than the normal spermatozoa. Thus, percentage of normal spermatozoa with condensation defects could be a predictive factor for the rate of fertilization in IVF. From diverse shapes of nucleus in AFM imaging, it could be predicted that defective nuclear shaping might be impeding the activity of some proteins/ biological motors, those regulate the proper Golgi spreading over peri-nuclear theca.

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“…Fuorescence microscopy of samples stained with acridine orange, aniline blue and toluidine blue, acridine orange-propidium iodide is used for identification of sperm nuclei with the abnormal chromatin condensation and the ploidy (Erenpreiss et al 2001, Erenpreisa et al 2002, Bungum et al 2007, Mahfouz et al 2009, Moskovtsev et al 2009, Lazaros et al 2011, Montjean et al 2012.…”

Section: Evaluation Of Sperm Morphologymentioning

confidence: 99%

Evaluation of morphological criteria of sperm quality before in vitro fertilization and intracytoplasmic sperm injection

Lasienë

Gedrimas

Vitkus

et al. 2013

Polish Journal of Veterinary Sciences

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The quality of sperm has a direct influence on the fertilization and developmental competence of embryos. In the literature we did not find defined criteria for evaluation of normal sperm parameters in various species of domestic mammals. Therefore we attempted to review evaluation of criteria of morphologically normal human sperm and their abnormalities. All sperm cells observed in the stained sample are classified as normal or abnormal. Any abnormalities in morphology of sperm have a negative effect on the outcome in in vitro fertilization and intracytoplasmic sperm injection. Abnormal sperm are categorized into subgroups according to the observed defects (concerning the head and/or midpiece and/or tail). Most morphologically abnormal sperm have multiple defects. This article can be considered as guideline for the manual of sperm quality evaluation in different species of domestic mammals.

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Assessment of sperm chromatin condensation and ploidy status using flow cytometry correlates to fertilization, embryo quality and pregnancy following in vitro fertilization

Cited by 35 publications

References 47 publications

Assessment of Chromatin Maturity in Human Spermatozoa: Useful Aniline Blue Assay for Routine Diagnosis of Male Infertility

Assessment of Chromatin Maturity in Human Spermatozoa: Useful Aniline Blue Assay for Routine Diagnosis of Male Infertility

An illustration of human sperm morphology and their functional ability among different group of subfertile males

Evaluation of morphological criteria of sperm quality before in vitro fertilization and intracytoplasmic sperm injection

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