Evaluation of morphological criteria of sperm quality before in vitro fertilization and intracytoplasmic sperm injection

Abstract

The quality of sperm has a direct influence on the fertilization and developmental competence of embryos. In the literature we did not find defined criteria for evaluation of normal sperm parameters in various species of domestic mammals. Therefore we attempted to review evaluation of criteria of morphologically normal human sperm and their abnormalities. All sperm cells observed in the stained sample are classified as normal or abnormal. Any abnormalities in morphology of sperm have a negative effect on the outcome in in vitro fertilization and intracytoplasmic sperm injection. Abnormal sperm are categorized into subgroups according to the observed defects (concerning the head and/or midpiece and/or tail). Most morphologically abnormal sperm have multiple defects. This article can be considered as guideline for the manual of sperm quality evaluation in different species of domestic mammals.

Selected steps of swine ICSI method to overcome seasonal effect and achieve acceptable early embryonic development, a preliminary study

“…Our results of two PN formation (49-55%) are similar with those of García-Roselló et al (2006a) (55.4-59.7%). Semen variation, sperm morphological abnormalities and poor sperm chromatin integrity have negative effect on the ICSI's outcome (Lasiene et al 2013). The selection of one boar and of an alternative to PVP, probably supported the results of the present study.…”

The effect of selected staining techniques on bull sperm morphometry

“…Sperm morphology continues to be an important parameter in predicting fertility in both humans and animals (Keel et al, 2002;Maree et al, 2010;Lasiene et al, 2013). One solution in evaluation of sperm morphology is computer-assisted sperm analysis (CASA), which reduces the subjectivism of morphological analysis (Gago et al, 1998), but these systems are relatively expensive and also not free of errors resulting from automation of the analysis process.…”

Evaluation of sperm morphometry of rabbits (Oryctolagus cuniculus f. domesticus)

“…This results in a small cellular nucleus and thus a small head, which renders routine evaluation of its morphology difficult (Bielańska‐Osuchowska & Sysa, 1998; Bielańska‐Osuchowska & Tishner, 2018; Shaman & Ward, 2006; Sharma & Agarwal, 2011). Sperm morphology remains an important factor in fertility evaluation (Keel et al., 2002; Lasiene et al., 2013; Maree et al., 2010; Oral et al., 2002). While other basic sperm parameters such as mobility and concentration are important, morphology seems to be the most significant, especially in artificial insemination, which is increasingly common (Singh et al., 2011).…”