Assessment of susceptible Culex quinquefasciatus larvae in Indonesia to different insecticides through metabolic enzymes and the histopathological midgut

Subahar, Rizal; Aulia, Annisa Putri; Yulhasri, Yulhasri; Felim, Ris Raihan; Susanto, Lisawati; Winita, Rawina; Bayani, Gulshan Fahmi El; Adugna, Tilahun

doi:10.1016/j.heliyon.2022.e12234

Cited by 9 publications

(9 citation statements)

References 49 publications

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“…This identification was further supported by the characteristic outdoor location and breeding site water types, as reported in previous studies (15,17). Furthermore, Aedes albopictus, like Aedes aegypti, can be found in clear standing water, although its preferred breeding locations differ (20).…”

Section: Discussionsupporting

confidence: 84%

“…Larval susceptibility was categorized as susceptible, moderately susceptible, or resistant based on mortality rates. Complete susceptibility was defined as 100% mortality, while mortality between 90-97% indicated moderate susceptibility, and mortality below 90% suggested low susceptibility or potential resistance to temephos insecticide (20).…”

Section: Discussionmentioning

confidence: 99%

“…Numerous studies have identified various factors influencing larval mosquito presence in breeding sites, including pH, water temperature, dissolved oxygen, turbidity, soluble solids, salinity, resistance, and nutrition (19)(20)(21). High dry-season temperatures might reduce breeding site availability, as observed in this study (25), and supported by other research (26).…”

Section: Discussionmentioning

confidence: 99%

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Entomological Survey and Temephos Susceptibility of Aedes, Culex and Anopheles in Naena Muktipura Mimika Papua

Novyan Lusiyana

2024

JKL

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Introduction: Arthropod-borne diseases are infectious diseases mediated by mosquitoes that have incurred a considerable health problem. The prevalence of this disease is notably high in some rural areas, such as Papua, due to some environmental conditions that support mosquitoes breeding sites and leads to high rate of disease transmission. Methods: This experimental research aims to determine the breeding site, mosquitos’ diversity, and susceptibility to temephos insecticide through an entomological survey to find out the breeding site of the larvae. The positive breeding sites of the larva were captured and identified in the laboratory. The temephos test were carried out for each species of larvae at dose 0,02 ppm for Aedes albopictus and Culex sp, and 0.25 ppm for Anopheles sp. Results and Discussion: Entomology survey showed that 5 of 27 stagnant water were positive breeding site of mosquito larvae. The following larvae were found: Aedes sp, Culex sp and Anopheles sp. Culex sp and Anopheles sp prefer dirty stagnant water covered with leaves, while Aedes albopictus likes puddles that are exposed to direct sunlight. The three species were known to be susceptible to temephos. Conclusion: There are three species of Diptera in Naena Muktipura village, with different characteristics of breeding site, but those mosquitoes are all susceptible to temephos.

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Section: Discussionsupporting

confidence: 84%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

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Entomological Survey and Temephos Susceptibility of Aedes, Culex and Anopheles in Naena Muktipura Mimika Papua

Novyan Lusiyana

2024

JKL

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“…Thus, compounds that block or stimulate these enzymes can cause metabolic imbalances, stunted growth, and even death in mosquito larvae. 38 Guppy fish's skin colour, survival rate, or swimming behaviour remained unchanged even after 120 minutes of exposure to chitinase. Therefore, the results confirm that chitinase is safe in terms of germination potential and toxicity to non-targeted organisms.…”

Section: Discussionmentioning

confidence: 95%

Mosquito Larvicidal Activity of Chitinase of Pseudomonas putida Mb 12 against the Human Vector Aedes aegypti

Paul¹,

Mathew²

2023

J. Pure Appl. Microbiol.

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The main dengue and Zika vector, Aedes aegypti, is a cosmotropic species. Since dengue fever cases have significantly increased in recent years, these organisms seem to be extremely detrimental. Synthetic pesticides are not biodegradable, are non-selective, and have adverse effects on beneficial organisms being handled in the vector management system. In the present study, the mosquitocidal potential of chitinase from P. putida Mb 12 was evaluated in an effort to identify risk-free options for the control of mosquitoes. Larvicidal toxicity of Pseudomonas putida Mb 12 chitinase were evaluated on IVth-instar larva of Ae. aegypti and their effect on acetylcholinesterase activity and glutathione S-transferase activity were studied. The early 4th instar larvae of Ae. aegypti were exposed to chitinase enzyme concentrations of 50U/mL, 100U/mL, 200U/mL, and 500U/mL for a period of 4 hours to assess their effectiveness. The results showed that as chitinase concentration increased, mosquito larvae mortality increased; after 4 hours, chitinase at 500 U/mL caused 100% mortality. After 4 hours of incubation, 200 U/mL was administered to achieve LC50 (which kills 50% of the exposed organisms), and after 3 hours, 500 U/mL was used to obtain LC90 values. During the study, it was discovered that different quantities of chitinase (100 U/mL, 200 U/mL, and 500 U/mL) inhibited 80% of the activity of the acetylcholinesterase enzyme. This study found that chitinase significantly increased glutathione S-transferase activity. Additionally, it was discovered that the chitinase treatment was non-hazardous to guppy fish. It was assumed that the P. putida Mb 12 chitinase tested was safe to employ in the aquatic habitat because no mortality was observed in the non-target organisms.

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“…Brie y, C. quinquefasciatus larvae (n = 200) of 3rd instar were transferred for ve recipients (200 mL) containing 99 mL of distilled water, and concentrations ranging from 5 to 25 µg/mL of the EO from T. riparia, and fenchone prepared in 1 mL of DMSO. Additionally, a concentration of 0.25 µg/mL α-cypermethrin was used as positive control, as previously described by (Subahar et al 2022). DMSO at 1% was used as negative control.…”

Section: Larvicidal Activitymentioning

confidence: 99%

Essential oil and fenchone extracted from Tetradenia riparia (Hochstetter.) Codd (Lamiaceae) induce oxidative stress in Culex quinquefasciatus larvae (Diptera: Culicidae) without causing lethal effects on non-target animals

de Oliveira,

Simões,

da Silva

et al. 2024

Environ Sci Pollut Res

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We investigated the larvicidal activity of the essential oil (EO) from Tetradenia riparia and its majority compound fenchone for controlling Culex quinquefasciatus larvae, focusing in reactive oxygen and nitrogen species (RONS), catalase (CAT), glutathione S-transferase (GST), acetylcholinesterase (AChE) activities, and total thiol content as oxidative stress indicators. Moreover, the lethal effect of EO and fenchone was evaluated against Anisops bouvieri, Diplonychus indicus, Danio rerio, and Paracheirodon axelrodi. The EO and fenchone (5 to 25 µg/mL) showed larvicidal activity (LC 50 from 16.05 to 18.94 µg/mL), followed by an overproduction of RONS, and changes in the activity of CAT, GST, AChE and total thiol content. The Kaplan-Meier followed by Log-rank (Mantel-Cox) analyses showed a 100% survival rate for A. bouvieri, D. indicus, D. rerio, and P. axelrodi when exposed to EO and fenchone (262.6 and 302.60 µg/mL), while α-cypermethrin (0.25 µg/mL) was extremely toxic to these non-target animals, causing 100% of death. These ndings emphasize that the EO from T. riparia and fenchone serve as suitable larvicides for controlling C. quinquefasciatus larvae, without imposing lethal effects on the non-target animals investigated.

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Assessment of susceptible Culex quinquefasciatus larvae in Indonesia to different insecticides through metabolic enzymes and the histopathological midgut

Cited by 9 publications

References 49 publications

Entomological Survey and Temephos Susceptibility of Aedes, Culex and Anopheles in Naena Muktipura Mimika Papua

Entomological Survey and Temephos Susceptibility of Aedes, Culex and Anopheles in Naena Muktipura Mimika Papua

Mosquito Larvicidal Activity of Chitinase of Pseudomonas putida Mb 12 against the Human Vector Aedes aegypti

Essential oil and fenchone extracted from Tetradenia riparia (Hochstetter.) Codd (Lamiaceae) induce oxidative stress in Culex quinquefasciatus larvae (Diptera: Culicidae) without causing lethal effects on non-target animals

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