Assessment of the Anti-Inflammatory, Antibacterial and Anti-Aging Properties and Possible Use on the Skin of Hydrogels Containing Epilobium angustifolium L. Extracts

Abstract

Epilobium angustifolium L. is an ethnomedicinal plant known as a medicinal plant in many regions of the world, among others, in various skin diseases. Despite the great interest in this plant, there are still few reports of biological activity of ready-made dermatological or cosmetical preparations containing the E. angustifolium extracts. The antioxidant, anti-ageing, anti-inflammatory, antibacterial properties and toxicity, wound healing, and skin permeation of topical hydrogels containing E. angustifolium extracts (HEas) was assessed. First, the plant extracts were prepared using three solvents: 70% (v/v) ethanol, 70% (v/v) isopropanol and water, next by preparing hydrogels witch by dry extracts (HEa-EtOH), (HEa-iPrOH) and (HEa-WA), respectively. Finally, the content of selected phenolic acids in the HEas was evaluated by high-performance liquid chromatography (HPLC). All the HEas were characterized by high antioxidant activity. The most increased antibacterial activity was observed for a strain of Streptococcus pneumoniae ATCC 49619, Escherichia coli, Enterococcus faecalis ATCC 29212, Enterococcus faecium, Sarcina lutea ATCC 9341 and Bacillus pseudomycoides, while the strains of Streptococcus epidermidis, Bacillus subtilis, and Staphylococcus aureus were the least sensitive. All the HEas showed a reduction in the activity of lipoxygenase enzymes, proteases, and inhibition of protein denaturation. The HEa-EtOH and HEa-iPrOH also enhanced the wound healing activity of HDF cells. Additionally, in vitro penetration studies were performed using the Franz diffusion cells. These studies showed that the active ingredients contained in E. angustifolium penetrate through human skin and accumulate in it. Furthermore, the hydrogels containing E. angustifolium extracts showed a broad spectrum of activity. Therefore, they can be considered as an interesting alternative for dermatologic and cosmetic preparations.

Phenolic Profile and Comparison of the Antioxidant, Anti-Ageing, Anti-Inflammatory, and Protective Activities of Borago officinalis Extracts on Skin Cells

“…Hence, compounds showing the ability to inhibit proteinases can play a significant role in inhibiting inflammation [ 21 ]. Literature data indicate that many biologically active compounds present in plants can inhibit the activity of these enzymes [ 23 , 24 ], so this work also assessed the possibility of proteinase inhibition. The results indicated that all four extracts can inhibit the activity of this enzyme in a manner dependent on both the concentration and type of extractant ( Figure 8 ).…”

New Ferulic Acid and Amino Acid Derivatives with Increased Cosmeceutical and Pharmaceutical Potential

“…The stability of all hydrogels and emulsions was tested as in the previous studies [ 29 , 30 ]. Briefly, the stability of all preparations was evaluated by centrifuge test.…”

Amino acid profiling in wild Chamaenerion angustifolium populations applying chemometric analysis

“…Thanks to these enzymes, nitrogen is metabolized from soil nitrates and their gradual transformation into secondary metabolites (hydroxycinnamic acids and flavonoids). Alanine is the first amino acid formed in the plants roots, then dicarboxylic amino acids (aspartic and glutamic acids) are formed after a relatively short time (Parthasarathy et al, 2019). The synthesis of diamino acids and aromatic amino acids occurs much later in leaves, apparently due to the amine groups of alanine and dicarboxylic amino acids as a result of transamination reactions (Santosh et al, 2021).…”

“…Moreover, the presence of fatty acids in fireweed herb (Granica et al, 2012) has been reported. Some amino acids were found in the lipophilic fraction of the aerial part of C. angustifolium (Abudeiyh et al, 2010;Polezhaeva et al, 2007). Other organic acids of C. angustifolium are also poorly studied; L-ascorbic acid was identified only in its aerial parts (Shikov et al, 2006).…”

“…Other organic acids of C. angustifolium are also poorly studied; L-ascorbic acid was identified only in its aerial parts (Shikov et al, 2006). The modern pharmacological studies confirmed antiproliferative (Vitalone et al, 2001), anti-inflammatory, antioxidant, antimicrobial (Nowak et al, 2022), immunomodulatory (Schepetkin et al, 2009), and other activities of fireweed (Granica et al, 2014;Vitalone and Allkanjari, 2018).…”