Assessment of the Bone Regenerative Process from Fibular Periosteum by in vivo Micro Computed Tomography

Mashimo, Takayuki; Saito, Tadahito; Shiratsuchi, Hiroshi; Iwata, Jun; Uryu, Takeshi; Tamagawa, Takaaki; Namaki, Shunsuke; Matsumoto, K; Kawashima, Shigeto; Mori, Yoshiyuki; Arai, Yoshinori; Honda, Kazumasa; Yonehara, Yoshiyuki

doi:10.2485/jhtb.22.391

Cited by 4 publications

(5 citation statements)

References 35 publications

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“…There is abundant evidence suggesting that fracture healing is impaired if periosteum is removed, or that otherwise critical-sized defects in a variety of bones can regrow if periosteum is retained (39)(40)(41)(42). There are also numerous studies reporting superior outcomes with cultured periosteal cells compared to other cell sources like bone marrow in terms of bone formation both in vitro and in vivo (20,43).…”

Section: Discussionmentioning

confidence: 99%

Heterogeneity of murine periosteum progenitors involved in fracture healing

Matthews

Novak

Sbrana

et al. 2021

eLife

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The periosteum is the major source of cells involved in fracture healing. We sought to characterize progenitor cells and their contribution to bone fracture healing. The periosteum is highly enriched for progenitor cells, including Sca1+ cells, CFU-F and label-retaining cells compared to the endosteum and bone marrow. Using lineage tracing, we demonstrate that αSMA identifies long-term, slow-cycling, self-renewing osteochondroprogenitors in the adult periosteum that are functionally important for bone formation during fracture healing. In addition, Col2.3CreER-labeled osteoblast cells contribute around 10% of osteoblasts, but no chondrocytes in fracture calluses. Most periosteal osteochondroprogenitors following fracture, can be targeted by αSMACreER. Previously identified skeletal stem cell populations were common in periosteum, but contained high proportions of mature osteoblasts. We have demonstrated that the periosteum is highly enriched for skeletal progenitor cells and there is heterogeneity in the populations of cells that contribute to mature lineages during periosteal fracture healing.

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Section: Discussionmentioning

confidence: 99%

Heterogeneity of murine periosteum progenitors involved in fracture healing

Matthews

Novak

Sbrana

et al. 2021

eLife

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“…We confirmed that the periosteum is highly enriched for cells with markers and phenotype of stem and progenitor cells. There is abundant evidence suggesting that fracture healing is impaired if periosteum is removed, or that otherwise critical-sized defects in a variety of bones can regrow if periosteum is retained (31)(32)(33)(34). There are also numerous studies reporting superior outcomes with cultured periosteal cells compared to other cell sources like bone marrow in terms of bone formation both in vitro and in vivo (19,35).…”

Section: Discussionmentioning

confidence: 99%

Heterogeneity of murine periosteum progenitors involved in fracture healing

Matthews

Novak

Sbrana

et al. 2020

Preprint

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The periosteum is the major source of cells involved in fracture healing. We sought to characterize differences in progenitor cell populations between periosteum and other bone compartments, and identify periosteal cells involved in fracture healing. The periosteum is highly enriched for progenitor cells, including Sca1+ cells, CFU-F and label-retaining cells. Lineage tracing with αSMACreER identifies periosteal cells that contribute to >80% of osteoblasts and ~40% of chondrocytes following fracture.A subset of αSMA+ cells are quiescent long-term injury-responsive progenitors. Ablation of αSMA+ cells impairs fracture callus formation. In addition, committed osteoblast-lineage cells contributed around 10% of osteoblasts, but no chondrocytes in fracture calluses. Most periosteal progenitors, particularly those that form osteoblasts, can be targeted by αSMACreER. We have demonstrated that the periosteum is highly enriched for skeletal stem and progenitor cells and there is heterogeneity in the populations of cells that contribute to mature lineages during periosteal fracture healing.

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“…We have previously investigated the properties of regenerative bone from the fibular periosteum used in the present study. 2) Our results showed that regenerative bone at 1 week was juvenile bone with a large amount of bone marrow, regenerative bone at 4 weeks was bone immediately after becoming mature, and regenerative bone at 8 weeks was completely mature. On comparing our present results with those of our previous study, we found that juvenile bone grafts such as RB1 resisted resorption and had high regenerative activity as compared with mature bone grafts such as RB8.…”

Section: Takayuki Mashimo Et Almentioning

confidence: 53%

“…1,2) Previous studies have demonstrated that vascularized periosteal grafts have excellent osteogenic capacity and promote the formation of bone in bone defects. [3][4][5][6] A recent study reported that cultured periosteum-derived cells show osteogenic capacity in bone defects.…”

Section: Introductionmentioning

confidence: 99%

A New Graft Material for Mandibular Bone Defect Repair Using Regenerative Bone from Periosteum

Mashimo

Saito

Shiratsuchi

et al. 2014

J. Hard Tissue Biology.

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The objective of this study was to evaluate the usefulness of regenerative bone from periosteum as a new graft material for the repair of bone defects. Regenerative bone at 1, 4, and 8 weeks (RB1, RB4 and RB8) was harvested from the fibular periosteum of donor rats as graft materials. These regenerative bones were transplanted into the mandibular bone defects of recipient rats in the experimental groups (RB1, RB4 and RB8 groups). Normal fibulas (NF), which were harvested from donor rats, were transplanted in the control groups (NF groups). Immediately, 2, and 4 weeks after transplantation, radiological and histological findings were evaluated. Bone healing was observed in all groups. In particular, the height and width ratios in the RB1 groups were significantly higher than those in the NF groups, and RB1 resisted resorption as compared with the other bone grafts. The bone mineral density in the RB1 groups was significantly less than that in the NF groups. These results indicate that regenerative bone from periosteum is a useful reconstructive material. The properties of regenerative bone after transplantation differ depending on the timing of regeneration.

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Assessment of the Bone Regenerative Process from Fibular Periosteum by in vivo Micro Computed Tomography

Cited by 4 publications

References 35 publications

Heterogeneity of murine periosteum progenitors involved in fracture healing

Heterogeneity of murine periosteum progenitors involved in fracture healing

Heterogeneity of murine periosteum progenitors involved in fracture healing

A New Graft Material for Mandibular Bone Defect Repair Using Regenerative Bone from Periosteum

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