Assessment of the “cross-bridge”-induced interaction of red blood cells by optical trapping combined with microfluidics

Lee, Kisung; Wagner, Christian; Priezzhev, Alexander V.

doi:10.1117/1.jbo.22.9.091516

Cited by 21 publications

(27 citation statements)

References 33 publications

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“…Therefore, a linear fitting was applied to the measured data as shown in Figure 4a. However, as suggested by a recent study [31], the "cross-bridging" mechanism is partly involved in RBC aggregation, thus the relationship may not be accurately described by one single model. For disaggregation measurement as shown in Figure 3b, after 40 s cell contact at an 80% overlapping area, a constant optical trapping force was applied to slowly drag one RBC away from the other cell until at least one RBC escaped from the trap.…”

Section: Interaction Energy Density Of Rbcs During Aggregation and DImentioning

confidence: 94%

Influence of Pulsed He–Ne Laser Irradiation on the Red Blood Cell Interaction Studied by Optical Tweezers

et al. 2019

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Optical Tweezers (OT), as a revolutionary innovation in laser physics, has been extremely useful in studying cell interaction dynamics at a single-cell level. The reversible aggregation process of red blood cells (RBCs) has an important influence on blood rheological properties, but the underlying mechanism has not been fully understood. The regulating effects of low-level laser irradiation on blood rheological properties have been reported. However, the influence of pulsed laser irradiation, and the origin of laser irradiation effects on the interaction between RBCs remain unclear. In this study, RBC interaction was assessed in detail with OT. The effects of both continuous and pulsed low-level He–Ne laser irradiation on RBC aggregation was investigated within a short irradiation period (up to 300 s). The results indicate stronger intercellular interaction between RBCs in the enforced disaggregation process, and both the cell contact time and the initial contact area between two RBCs showed an impact on the measured disaggregation force. Meanwhile, the RBC aggregation force that was independent to measurement conditions decreased after a short time of pulsed He–Ne laser irradiation. These results provide new insights into the understanding of the RBC interaction mechanism and laser irradiation effects on blood properties.

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Section: Interaction Energy Density Of Rbcs During Aggregation and DImentioning

confidence: 94%

Influence of Pulsed He–Ne Laser Irradiation on the Red Blood Cell Interaction Studied by Optical Tweezers

et al. 2019

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“…The flow can be generated by the controlled movement of the sample stage or by micro-pumping the fluid into a microfluidic chamber. For a small displacement within the trapping range, the trapping force acts against the dragging force described by Stokes' law [78]:…”

Section: Trapping Force Calibrationmentioning

confidence: 99%

“…The development of advanced trapping approaches has encouraged the design of novel experimental methodologies that integrate the OTs with microfluidic platforms or other microscopic techniques for in-depth investigation of RBC interaction dynamics in the well-controlled single-cell environment. Based on HOTs and the fact that a microfluidic platform consists of two connected chambers as shown in Figure 11, the RBC aggregation force can be ingeniously measured in changing solutions (plasma, phosphate buffer saline, protein solutions of fibrinogen and/or albumin) with designed order to explore the aggregation mechanism [78]. The interesting observation that RBC adhesion is strongly dependent on the initial aggregate-forming solution provides new evidence of the involvement of the "cross-bridging" mechanism in the RBC aggregation process.…”

Section: Evaluation Of Dynamic Cell-cell Interaction Between Rbcsmentioning

confidence: 99%

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Optical Tweezers in Studies of Red Blood Cells

Zhu

Avsievich

Попов

et al. 2020

Cells

106

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Optical tweezers (OTs) are innovative instruments utilized for the manipulation of microscopic biological objects of interest. Rapid improvements in precision and degree of freedom of multichannel and multifunctional OTs have ushered in a new era of studies in basic physical and chemical properties of living tissues and unknown biomechanics in biological processes. Nowadays, OTs are used extensively for studying living cells and have initiated far-reaching influence in various fundamental studies in life sciences. There is also a high potential for using OTs in haemorheology, investigations of blood microcirculation and the mutual interplay of blood cells. In fact, in spite of their great promise in the application of OTs-based approaches for the study of blood, cell formation and maturation in erythropoiesis have not been fully explored. In this review, the background of OTs, their state-of-the-art applications in exploring single-cell level characteristics and bio-rheological properties of mature red blood cells (RBCs) as well as the OTs-assisted studies on erythropoiesis are summarized and presented. The advance developments and future perspectives of the OTs’ application in haemorheology both for fundamental and practical in-depth studies of RBCs formation, functional diagnostics and therapeutic needs are highlighted.

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“…In the model of bridges, the interaction is described by the forces that arise due to adsorption of macromolecules at the surface of membranes of adjacent erythrocytes [15]. To date there are serious arguments in favour of both models [14,16]. However, making a final decision on the correctness of model considerations requires additional studies.…”

Section: Introductionmentioning

confidence: 99%

Interaction of erythrocytes in the process of pair aggregation in blood samples from patients with arterial hypertension and healthy donors: measurements with laser tweezers

Ermolinskiy

Луговцов

Maslyanitsina

et al. 2018

J-BPE

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Two-channel laser tweezers are used to measure the interaction kinetics of two erythrocytes at the initial stage of aggregation, i.e., the formation of a pair aggregate in vitro. The study of erythrocytes interaction is important both for understanding the fundamental aggregation mechanisms and for evaluating the differences in kinetics and dynamics of aggregation, depending on the presence or absence of diseases that disturb the blood flow parameters and, therefore, the oxygen supply to tissues. We analyse the kinetics and dynamics of pair aggregation of erythrocytes in blood samples from more than 60 patients with arterial hypertension, as well as from a group of healthy donors. The results show that both kinetics and dynamics of erythrocyte aggregation are changed in the presence of the considered pathology.

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Assessment of the “cross-bridge”-induced interaction of red blood cells by optical trapping combined with microfluidics

Cited by 21 publications

References 33 publications

Influence of Pulsed He–Ne Laser Irradiation on the Red Blood Cell Interaction Studied by Optical Tweezers

Influence of Pulsed He–Ne Laser Irradiation on the Red Blood Cell Interaction Studied by Optical Tweezers

Optical Tweezers in Studies of Red Blood Cells

Interaction of erythrocytes in the process of pair aggregation in blood samples from patients with arterial hypertension and healthy donors: measurements with laser tweezers

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