In this work, we compare the blood aggregation parameters measured in vitro by laser aggregometry and optical trapping techniques in blood samples with the parameters of blood rheology measured in vivo by digital capillaroscopy in the nail bed capillaries of patients suffering from the hypertension and coronary heart disease. We show that the alterations of the parameters measured in vivo and in vitro for patients with different stages of these diseases are interrelated. Good agreement between the results obtained with different techniques, and their applicability for the diagnostics of abnormalities of rheological properties of blood are demonstrated.

Two-channel laser tweezers are used to measure the interaction kinetics of two erythrocytes at the initial stage of aggregation, i.e., the formation of a pair aggregate in vitro. The study of erythrocytes interaction is important both for understanding the fundamental aggregation mechanisms and for evaluating the differences in kinetics and dynamics of aggregation, depending on the presence or absence of diseases that disturb the blood flow parameters and, therefore, the oxygen supply to tissues. We analyse the kinetics and dynamics of pair aggregation of erythrocytes in blood samples from more than 60 patients with arterial hypertension, as well as from a group of healthy donors. The results show that both kinetics and dynamics of erythrocyte aggregation are changed in the presence of the considered pathology.

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