Optical assessment of alterations of microrheologic and microcirculation parameters in cardiovascular diseases

Луговцов, А.Е.; Gurfinkel, Yu. I.; Ermolinskiy, Petr B.; Maslyanitsina, A.I.; Dyachuk, L I; Priezzhev, Alexander V.

doi:10.1364/boe.10.003974

Cited by 18 publications

(10 citation statements)

References 21 publications

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“…The second one included mixing the plasma with PBS and NaCl in order to achieve different osmolarities 150 to 500 mOsm/l and then adding RBC at 40% hematocrit. Next, three optical techniques described in more detail in the work [12] were used to study the cells in vitro.…”

Section: Blood Samplesmentioning

confidence: 99%

Study by optical techniques of the dependence of aggregation parameters of human red blood cells on their deformability

Ermolinsky

Луговцов

Priezzhev

2020

J-BPE

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Blood microcirculation in human body is greatly dependent on the microrheologic properties of red blood cells. The aim of this work is to identify the relationship between the deformability of these cells and their aggregation properties, both of which are the key factors for the blood flow. Laser diffractometry, diffuse light scattering and laser tweezers were implemented for in vitro measurements. Different osmolarity of plasma (150-500 mOsm/l) and concentrations of glutaraldehyde (up to 0.004%) were used to change the deformability of healthy red blood cells in vitro. The results show that with the cells becoming more rigid some aggregation parameters (e.g. the fraction of aggregated cells) decrease, while some of them (e.g. the hydrodynamic strength of the aggregate) stay unchanged. For example, after incubation in 0.004% glutaraldehyde solution the erythrocyte deformability drops by 19 ± 2% and this leads to a decrease by 77 ± 4% in the aggregation index. This means that there is a connection between cell deformability and the formation of the aggregates, however the relationship is less pronounced and more complex for the disaggregation process.

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Section: Blood Samplesmentioning

confidence: 99%

Study by optical techniques of the dependence of aggregation parameters of human red blood cells on their deformability

Ermolinsky

Луговцов

Priezzhev

2020

J-BPE

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“…LTs are a powerful laser technique allowing to trap living cells to manipulate them without mechanical contact [7,20]. Recently, they proved to be a very useful tool for studying the characteristics of RBC interaction [21,22], to quantify aggregation forces [23] as well as for the complementation of other methods [24,25]. In this work, LTs were used to study the interaction of pair-aggregating RBCs in autologous plasma and in the dextran 70 kDa (50 mg/mL) solution.…”

Section: Introductionmentioning

confidence: 99%

Effect of Red Blood Cell Aging In Vivo on Their Aggregation Properties In Vitro: Measurements with Laser Tweezers

et al. 2020

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Red blood cell (RBC) aggregation highly influences hemorheology and blood microcirculation in the human body. The aggregation properties of RBCs can vary due to numerous factors, including RBC age. The aim of this work was to estimate in vitro the differences in the RBC aggregation properties of different RBC age populations in single-cell experiments using laser tweezers. RBCs from five healthy volunteers were separated into four subpopulations by Percoll density gradient centrifugation. Each subpopulation of the RBC was separately resuspended in autologous plasma or dextran 70 kDa (50 mg/mL). The aggregation force between the single cells was measured with holographic laser tweezers. The obtained data demonstrated an enhancement of RBC aggregation force in doublets with age: the older the cells, the higher the aggregation force. The obtained data revealed the differences between the aggregation and aggregability of RBC in dependence of the RBC in vivo age.

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“…Flow cytometry is a well-known method for studying parameters of the interactions between different components of blood [ 44 ]. Optical trapping proved to be a very useful approach for studying peculiarities of RBC interaction, microrheology, and biomechanics [ 18 , 45 , 46 , 47 , 48 ] as well as for quantifications of a number of proaggregant macromolecules adsorbed on the surface of RBC [ 49 ]. In the present work, we implemented advanced optical techniques including the diffuse light scattering technique (laser aggregometry), flow cytometry, and optical (laser) tweezers combined with microfluidics for the assessment of novel features describing the interactions between blood plasma protein fibrinogen and the RBC membrane.…”

Section: Introductionmentioning

confidence: 99%

“…Enhanced fibrinogen-induced RBC aggregation occurs in a variety of infectious diseases [14,15] and genetic disorders [16]. Abnormalities in RBC aggregation also accompany cardiovascular diseases such as arterial hypertension [17,18], atherosclerosis [19], cardiac infarction, and ischemia [20], as well as during metabolic disorders such as diabetes mellitus [21,22]. During COVID-19 infection, pathological levels of fibrin and fibrinogen result in elevated membrane fragility and the pathological elasticity of erythrocytes [23].…”

Section: Introductionmentioning

confidence: 99%

Assessment of Fibrinogen Macromolecules Interaction with Red Blood Cells Membrane by Means of Laser Aggregometry, Flow Cytometry, and Optical Tweezers Combined with Microfluidics

et al. 2020

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An elevated concentration of fibrinogen in blood is a significant risk factor during many pathological diseases, as it leads to an increase in red blood cells (RBC) aggregation, resulting in hemorheological disorders. Despite the biomedical importance, the mechanisms of fibrinogen-induced RBC aggregation are still debatable. One of the discussed models is the non-specific adsorption of fibrinogen macromolecules onto the RBC membrane, leading to the cells bridging in aggregates. However, recent works point to the specific character of the interaction between fibrinogen and the RBC membrane. Fibrinogen is the major physiological ligand of glycoproteins receptors IIbIIIa (GPIIbIIIa or αIIββ3 or CD41/CD61). Inhibitors of GPIIbIIIa are widely used in clinics for the treatment of various cardiovascular diseases as antiplatelets agents preventing the platelets’ aggregation. However, the effects of GPIIbIIIa inhibition on RBC aggregation are not sufficiently well studied. The objective of the present work was the complex multimodal in vitro study of the interaction between fibrinogen and the RBC membrane, revealing the role of GPIIbIIIa in the specificity of binding of fibrinogen by the RBC membrane and its involvement in the cells’ aggregation process. We demonstrate that GPIIbIIIa inhibition leads to a significant decrease in the adsorption of fibrinogen macromolecules onto the membrane, resulting in the reduction of RBC aggregation. We show that the mechanisms underlying these effects are governed by a decrease in the bridging components of RBC aggregation forces.

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Optical assessment of alterations of microrheologic and microcirculation parameters in cardiovascular diseases

Cited by 18 publications

References 21 publications

Study by optical techniques of the dependence of aggregation parameters of human red blood cells on their deformability

Study by optical techniques of the dependence of aggregation parameters of human red blood cells on their deformability

Effect of Red Blood Cell Aging In Vivo on Their Aggregation Properties In Vitro: Measurements with Laser Tweezers

Assessment of Fibrinogen Macromolecules Interaction with Red Blood Cells Membrane by Means of Laser Aggregometry, Flow Cytometry, and Optical Tweezers Combined with Microfluidics

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