Assessment of the Extent of Starch Dissolution in Dimethyl Sulfoxide by <sup>1</sup>H NMR Spectroscopy

Schmitz, Sarah; Dona, Anthony C.; Castignolles, Patrice; Gilbert, Robert G.; Gaborieau, Marianne

doi:10.1002/mabi.200800244

Cited by 101 publications

(75 citation statements)

References 61 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Moreover, those starch molecules that do dissolve in water, especially the amylose ones, are ready to be retrograded due to the reassociation of these molecules with each other or with itself [207]. DMSO-d 6 has been shown to be a suitable solvent that can fully dissolve starch molecules [150]. The dissolution mechanism of starch granules in anhydrous DMSOd 6 is different from that in water.…”

Section: Nuclear Magnetic Resonancementioning

confidence: 99%

Causal Relations Among Starch Biosynthesis, Structure, and Properties

Wang

Henry

Gilbert

2014

Springer Science Reviews

Self Cite

View full text Add to dashboard Cite

Starch, an important carbohydrate with widespread applications in human foods, animal feeds, and many industrial products, is synthesized by plants by the action of a complex system involving many enzymes. The differing activities of these enzymes contribute to variations in starch structure among different plant species, botanical organs, and genetic backgrounds, and thus affect the physicochemical properties and end-use functions of starch. The demand for starches with particular functional properties is increasing, but the ability to produce novel starches is still limited. Starches with specific properties can potentially be produced by biotechnical modification of the starch biosynthetic pathway; however, this requires further understanding of the starch biosynthesis-structureproperties relationships. This review summarizes the state of the art in the understanding of these causal relationships: the roles of the main starch-synthesizing enzymes on starch structure, hierarchical structure of starch, advanced molecular structure characterization methods, and impact of starch structure on some functional properties. A better understanding of these relationships among starch biosynthesis, structure, and properties provides direction for genetic modification and targeted breeding programs to produce starch with desired characteristics.

show abstract

Section: Nuclear Magnetic Resonancementioning

confidence: 99%

Causal Relations Among Starch Biosynthesis, Structure, and Properties

Wang

Henry

Gilbert

2014

Springer Science Reviews

Self Cite

View full text Add to dashboard Cite

show abstract

“…5 mg of arabinoxylan or β-glucan was dissolved at 80°C overnight in 650 µL of d6-DMSO containing 0.5 wt % LiBr (Schmitz, Dona, Castignolles, Gilbert & Gaborieau, 2009). When the samples were cooled to room temperature, sodium 3-(trimethylsilyl) propionate-2,2,3,3-d4 (TSP) in D2O was added as an internal standard.…”

Section: H-nmrmentioning

confidence: 99%

Microstructure and mechanical properties of arabinoxylan and (1,3;1,4)-β-glucan gels produced by cryo-gelation

López-Sánchez

Wang

Zhang

et al. 2016

Carbohydrate Polymers

View full text Add to dashboard Cite

Microstructure and mechanical properties of arabinoxylan and (1,3;1,4)-beta;-glucan gels produced by cryogelation.Carbohydrate Polymers http://dx.doi.org/10. 1016/j.carbpol.2016.06.038 This is a PDF file of an unedited manuscript that has been accepted for publication. As a service to our customers we are providing this early version of the manuscript. The manuscript will undergo copyediting, typesetting, and review of the resulting proof before it is published in its final form. Please note that during the production process errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain. AbstractThe interactions between heteroxylans and mixed linkage glucans determine the architecture and mechanical properties of cereal endosperm cell walls. In this work hydrogels made of cross-linked arabinoxylan with addition of β-glucan were synthesised by cryogelation as a biomimetic tool to investigate endosperm walls. Molecular and microstructural properties were characterised by nuclear magnetic resonance ( 13 C NMR), scanning electron microscopy (SEM) and immunolabelling/confocal laser scanning 2 microscopy (CLSM). The response to mechanical stress was studied by compressionrelaxation experiments. The hydrogels consisted of a scaffold characterised by dense walls interconnected by macropores with both hemicelluloses co-localised and homogeneously distributed. The gels showed a high degree of elasticity reflected in their ability to resist compression without developing cracks and recover 60-80% of their original height. Our results highlight the compatibility of these hemicelluloses to coexist in confined environments such as cell walls and their potential role in determining mechanical properties in the absence of cellulose.

show abstract

“…This problem has been considered in detail by several authors. [70][71][72] In brief, the initial extraction needs to use a solvent system which is effective at breaking hydrogen bonding while unlikely to cause chemical degradation; dimethyl sulfoxide with a small amount of lithium bromide is held to be optimal in this regard. Multi-step procedures are then required to remove lipids, proteins, and non-starch polysaccharides.…”

Section: Techniques and Assumptionsmentioning

confidence: 99%

Characterization Methods for Starch-Based Materials: State of the Art and Perspectives

Witt

Gilbert

2013

Aust. J. Chem.

Self Cite

View full text Add to dashboard Cite

Improving starch-containing materials, whether food, animal feed, high-tech biomaterials, or engineering plastics, is best done by understanding how biosynthetic processes and any subsequent processing control starch structure, and how this structure controls functional properties. Starch structural characterization is central to this. This review examines how information on the three basic levels of the complex multi-scale structure of starch -individual chains, the branching structure of isolated molecules, and the way these molecules form various crystalline and amorphous arrangements -can be obtained from experiment. The techniques include fluorophore-assisted carbohydrate electrophoresis, multipledetector size-exclusion chromatography, and various scattering techniques (light, X-ray, and neutron). Some examples are also given to show how these data provide mechanistic insight into how biosynthetic processes control the structure and how the various structural levels control functional properties.

show abstract

Assessment of the Extent of Starch Dissolution in Dimethyl Sulfoxide by ¹H NMR Spectroscopy

Cited by 101 publications

References 61 publications

Causal Relations Among Starch Biosynthesis, Structure, and Properties

Causal Relations Among Starch Biosynthesis, Structure, and Properties

Microstructure and mechanical properties of arabinoxylan and (1,3;1,4)-β-glucan gels produced by cryo-gelation

Characterization Methods for Starch-Based Materials: State of the Art and Perspectives

Contact Info

Product

Resources

About

Assessment of the Extent of Starch Dissolution in Dimethyl Sulfoxide by 1H NMR Spectroscopy

Cited by 101 publications

References 61 publications

Causal Relations Among Starch Biosynthesis, Structure, and Properties

Causal Relations Among Starch Biosynthesis, Structure, and Properties

Microstructure and mechanical properties of arabinoxylan and (1,3;1,4)-β-glucan gels produced by cryo-gelation

Characterization Methods for Starch-Based Materials: State of the Art and Perspectives

Contact Info

Product

Resources

About

Assessment of the Extent of Starch Dissolution in Dimethyl Sulfoxide by ¹H NMR Spectroscopy