Assessment of the intracellular distribution of copper in liver specimens from cats

Yamkate, Punyamanee; Gold, Randi M.; Twedt, David C.; Suchodolski, Jan S.; Steiner, Jöerg M.; Lidbury, Jonathan A.

doi:10.1371/journal.pone.0264003

Cited by 3 publications

(2 citation statements)

References 31 publications

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“…However, based on previous studies, there is considerable doubt concerning the validity of this RI. 21,33 In contrast to the quantitative findings, positive rhodanine staining was found in only one specimen. The reported copper score was 2/5 in this specimen, with accumulation in the centrilobular and midzonal areas.…”

Section: Discussionmentioning

confidence: 77%

Quantitative bioimaging of copper in frozen liver specimens from cats using laser ablation-inductively coupled plasma-mass spectrometry

Yamkate

Funke

Steiger

et al. 2023

Journal of Feline Medicine and Surgery

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Objectives The aim of this study was to assess laser ablation-inductively coupled plasma-mass spectrometry (LA-ICP-MS) as a tool for measuring concentrations and determining accumulation of copper in frozen liver specimens from cats. Methods Six frozen liver specimens were evaluated by qualitative copper staining and quantitative flame atomic absorption spectroscopy. Tissue specimens were cryo-sectioned and quantitative bioimaging of copper was performed using LA-ICP-MS. Results were compared with those obtained using conventional methods. Results Of the six specimens, only one showed positive staining for copper with rhodanine. Using flame atomic absorption spectroscopy (FAAS), one specimen showed a deficient copper level (<100 µg/g dry weight), two specimens had copper within the reference interval (RI; 150–180 µg/g) and three specimens had copper concentrations above the RI. Bioimaging from LA-ICP-MS showed inhomogeneous distribution of hepatic copper. The areas with dense copper accumulation were represented as hotspots in the liver specimens. Hepatic copper quantification by LA-ICP-MS correlated well with copper quantified by FAAS ( r = 0.96, P = 0.002). Conclusions and relevance Our findings suggest that quantitative bioimaging by LA-ICP-MS could be used to demonstrate the distribution and concentration of copper in frozen liver specimens from cats. The distribution of copper in these specimens was inhomogeneous with dense accumulation represented as hotspots on tissue sections. A positive correlation of hepatic copper concentrations determined by LA-ICP-MS and FAAS was found. Further studies to establish an RI for hepatic copper using this technique and to further determine its clinical utility are warranted.

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Section: Discussionmentioning

confidence: 77%

Quantitative bioimaging of copper in frozen liver specimens from cats using laser ablation-inductively coupled plasma-mass spectrometry

Yamkate

Funke

Steiger

et al. 2023

Journal of Feline Medicine and Surgery

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“…A change in liver size is often an indicator of liver disease [ 58 ]. Common cat liver diseases include liver fibrosis, poisoning, copper-related liver conditions, and fatty liver [ 59 ]. Feline hepatic lipidosis (FHL), also termed feline fatty liver syndrome, is the most common liver disease [ 60 , 61 ].…”

Section: Probiotics and Liver And Kidney Healthmentioning

confidence: 99%

Probiotics and Cat Health: A Review of Progress and Prospects

Zha,

Zhu,

Chen

2024

Microorganisms

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Cats are increasingly favored as companion animals; their health has drawn widespread attention. Given the continuous improvements in the required living standards of both humans and animals, inflammatory bowel disease, allergies, diarrhea, constipation, periodontal disease, obesity, diabetes, and other health issues have become recognized as valid pet problems. Antibiotics are commonly used to treat pet diseases, greatly improving animal health. However, antibiotic abuse is common, especially when seeking to treat bacterial infections. Probiotics are beneficial microorganisms that may be directly ingested in food or as feed additives; they improve the intestinal microflora balance, enhance immunity, and ensure healthy growth. However, cat data are usually inferred from reports on dogs or humans; cat research remains preliminary in nature. Therefore, we here describe the current understanding of how probiotics improve cat health, facilitating the further development and application of probiotics for cats.

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Multicolor fluorescence regulation of N doped silicon quantum dots and the multiple applications

Wang¹,

Yu²,

Li³

et al. 2023

Sensors and Actuators B: Chemical

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Assessment of the intracellular distribution of copper in liver specimens from cats

Cited by 3 publications

References 31 publications

Quantitative bioimaging of copper in frozen liver specimens from cats using laser ablation-inductively coupled plasma-mass spectrometry

Quantitative bioimaging of copper in frozen liver specimens from cats using laser ablation-inductively coupled plasma-mass spectrometry

Probiotics and Cat Health: A Review of Progress and Prospects

Multicolor fluorescence regulation of N doped silicon quantum dots and the multiple applications

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