Assessment of the Long-Term Shedding Pattern of<i>Salmonella</i>Serovar<i>Choleraesuis</i>Following Experimental Infection of Neonatal Piglets

Anderson, Robin C.; Genovese, K. J.; Harvey, Roger B.; Stanker, Larry H.; DeLoach, John R.; Nisbet, David J.

doi:10.1177/104063870001200310

Cited by 24 publications

(21 citation statements)

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“…1 In the present study, none of the isolates were S. choleraesuis. Although toxic to S. choleraesuis, tetrathionate has been suggested as the medium of choice for enrichment of other Salmonella species (other than S. choleraesuis) in fecal specimens of carrier animals.…”

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confidence: 78%

“…Numerous preenrichments, particularly peptone water, have been used for culture of salmonellae. 1 When culturing samples from swine and their environment for Salmonella enterica serovar Choleraesuis, GN Hajna broth has been reported to be superior to other selective enrichments. 1,2,6-8 However, when culturing for salmonellae other than Salmonella Choleraesuis, tetrathionate broth has been routinely utilized for enrichment.…”

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confidence: 99%

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Comparison of GN Hajna and Tetrathionate as Initial Enrichment for Salmonellae Recovery from Swine Lymph Nodes and Cecal Contents Collected at Slaughter

et al. 2001

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An epidemiologic survey was conducted to determine the prevalence of salmonellae in swine from 5 farms of an integrated swine operation. The purpose of this study was to evaluate the recovery efficiencies for salmonellae from swine lymph nodes and cecal contents when GN Hajna and tetrathionate were compared as initial enrichments. Salmonellae were isolated from 61% of 645 pigs at slaughter; 324 positive cultures were from lymph nodes, and 224 were from cecal contents. Frequently, pigs had salmonellae isolated from both the lymph nodes and cecal contents. Total isolations, regardless of source, were similar for GN Hajna (247) and tetrathionate (301). There was no difference ( P > 0.05) in the number of isolations from lymph nodes when GN Hajna enrichment was compared with tetrathionate enrichment (174 vs. 150). However, there was a significant ( P > 0.05) advantage of utilizing tetrathionate when compared with GN Hajna for isolations from cecal contents (151 vs. 73).

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confidence: 78%

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Comparison of GN Hajna and Tetrathionate as Initial Enrichment for Salmonellae Recovery from Swine Lymph Nodes and Cecal Contents Collected at Slaughter

et al. 2001

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“…Studies of swine fecal samples subjected to multiple methods of selective enrichment for isolation of Salmonella have demonstrated that no culture method is 100% sensitive and that the serotypes isolated from the same sample differed by the culture method used, suggesting that serotypes differ in their susceptibilities to selective agents in enrichment media (24,49,54). The Salmonella serotype Choleraesuis, which is associated with swine, has been demonstrated to be sensitive to selective enrichment methods (2,54). Salmonella Choleraesuis was not isolated from any of the 4,560 carcasses sampled during this study, suggesting that the selective enrichment used in this study may not be suitable for the isolation of this serotype.…”

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confidence: 99%

“…The number of confirmed Salmonella was determined for each sample by multiplying the number of presumptive Salmonella colonies by the percentage of overnight cultures confirmed to contain Salmonella by invA PCR (9). The number of confirmed Salmonella was then reported as log CFU per 100 cm 2 . The lower limit of detection for prescald carcass samples was 1.4 log CFU/100 cm 2 .…”

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Prevalence, Enumeration, Serotypes, and Antimicrobial Resistance Phenotypes of Salmonella enterica Isolates from Carcasses at Two Large United States Pork Processing Plants

Schmidt

Brichta-Harhay

Kalchayanand

et al. 2012

Appl Environ Microbiol

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The objective of this study was to characterize Salmonella enterica contamination on carcasses in two large U.S. commercial pork processing plants. The carcasses were sampled at three points, before scalding (prescald), after dehairing/polishing but before evisceration (preevisceration), and after chilling (chilled final). The overall prevalences of Salmonella on carcasses at these three sampling points, prescald, preevisceration, and after chilling, were 91.2%, 19.1%, and 3.7%, respectively. At one of the two plants, the prevalence of Salmonella was significantly higher (P < 0.01) for each of the carcass sampling points. The prevalences of carcasses with enumerable Salmonella at prescald, preevisceration, and after chilling were 37.7%, 4.8%, and 0.6%, respectively. A total of 294 prescald carcasses had Salmonella loads of >1.9 log CFU/100 cm 2 , but these carcasses were not equally distributed between the two plants, as 234 occurred at the plant with higher Salmonella prevalences. Forty-one serotypes were identified on prescald carcasses with Salmonella enterica serotypes Derby, Typhimurium, and Anatum predominating. S. enterica serotypes Typhimurium and London were the most common of the 24 serotypes isolated from preevisceration carcasses. The Salmonella serotypes Johannesburg and Typhimurium were the most frequently isolated serotypes of the 9 serotypes identified from chilled final carcasses. Antimicrobial susceptibility was determined for selected isolates from each carcass sampling point. Multiple drug resistance (MDR), defined as resistance to three or more classes of antimicrobial agents, was identified for 71.2%, 47.8%, and 77.5% of the tested isolates from prescald, preevisceration, and chilled final carcasses, respectively. The results of this study indicate that the interventions used by pork processing plants greatly reduce the prevalence of Salmonella on carcasses, but MDR Salmonella was isolated from 3.2% of the final carcasses sampled.

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“…Choleraesuis has directly been isolated from the lung, liver, spleen, and other organs of diseased pigs [15,16]. In several recent studies of experimental infection of pigs with S. Choleraesuis, Rappaport Vassiliadis broth (RV) [19] was used as the enrichment broth [1,10,16]. Besides, Hajnatetrathionate broth (HTT) has widely been used as an enrichment broth for Salmonella in Japan [2,4,13,14].…”

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Enrichment for Isolating Salmonella Choleraesuis and other Salmonella spp. from Pigs

et al. 2003

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ABSTRACT. The growth of Salmonella Choleraesuis was examined in Rappaport Vassiliadis broth (RV) and Hajna-tetrathionate broth (HTT) at 37 and 42°C. As the enrichment in RV at 37°C was satisfactory for isolating S. Choleraesuis, we used this enrichment for isolation from the samples collected from 15 asymptomatic pigs reared on a S. Choleraesuis contaminated farm. S. Choleraesuis was frequently isolated from six pigs (40.0%) under field conditions. The isolation of other Salmonella serovars than S. Choleraesuis was attempted by using both RV enrichment at 37°C and HTT enrichment at 42°C. Salmonella organisms were isolated from 156 (44.8%) of 348 fecal samples and more frequently with HTT at 42°C (43.4%) than with RV at 37°C (20.9%). If other serovars in addition to S. Choleraesuis are to be surveyed, HTT enrichment should be used in combination with RV enrichment.

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Assessment of the Long-Term Shedding Pattern ofSalmonellaSerovarCholeraesuisFollowing Experimental Infection of Neonatal Piglets

Cited by 24 publications

References 11 publications

Comparison of GN Hajna and Tetrathionate as Initial Enrichment for Salmonellae Recovery from Swine Lymph Nodes and Cecal Contents Collected at Slaughter

Comparison of GN Hajna and Tetrathionate as Initial Enrichment for Salmonellae Recovery from Swine Lymph Nodes and Cecal Contents Collected at Slaughter

Prevalence, Enumeration, Serotypes, and Antimicrobial Resistance Phenotypes of Salmonella enterica Isolates from Carcasses at Two Large United States Pork Processing Plants

Enrichment for Isolating Salmonella Choleraesuis and other Salmonella spp. from Pigs

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