Assessment of the Physicochemical and Microbiological Quality of Palm Oil Mill Effluent (POME) and Soil in Aluu, Rivers State, Nigeria

Williams, Janet Olufunmilayo

doi:10.9734/ajob/2018/41855

Cited by 3 publications

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“…Pure cultures of the organism were obtained from inoculation and incubation of soil samples using nutrient Agar. Pure cultures were obtained by continuous subculturing (22,23). Isolates were inoculated into broth culture [19,24].…”

Section: Source Of Microorganisms (Pseudomonas Aeruginosa)mentioning

confidence: 99%

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Bioremediation Potential of Pseudomonas aeruginosa KX828570 on Crude Oil Spill Polluted Marshland and Terrestrial Soil Treated with Oil Spill Dispersant

Nrior

Inweregbu

2019

JAMB

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Aim: To investigate bioremediation potentiality of Pseudomonas aeruginosa KX828570 on crude oil Polluted Marshland and Terrestrial Soil treated with oil spill dispersant Study Design: The study employs experimental design, statistical analysis of data and interpretation. Place and Duration of the Study: Soil samples were collected from K-Dere, Gokana L.G.A, and were transported to the Microbiology Laboratory of Rivers State University, Port Harcourt, Nigeria for analyses while Oil spill dispersant (OSD/LT and OSD/Seacare) were from Barker and Hughes Nig Ltd (formally mil park Nigeria limited), all in Rivers state, Nigeria. This investigation study lasted for 28 days and sampling was done every 7day period. Methodology: Eight experimental set up were carried out using Pseudomonas aeruginosa KX828570 as the bio-augmenting organism in terrestrial and marshland soil contaminated with two Oil spill dispersant (OSD/LT and OSD/Seacare) separately. Controls for the two soil types were made without organism and treatment. Its bioremediation potential on the pollutants and two types of soil were monitored for 28 days. The setup was tilled twice a week to provide moisture and more oxygen for the organisms to thrive. Analysis of pH, Temperature, Moisture content, Total Hydrocarbon Content, Total Heterotrophic Bacteria, Dispersant Utilizing Bacteria was carried out at weekly intervals. Results: The pH of both soils ranged from 5.75 to 7.37; temperature from 270C to 340C; moisture content 03 to 0.6 across the soil samples. Total Hydrocarbon Content (THC) showed a steady decline from day 1 – 28. The percentage (%) bioremediation rates of polluted soils were as follows: Terrestrial soil+OSD/Seacare+Pseudomonas aeruginosa KX828570 69.4% > Terrestrial soil+OSD/LT+ Pseudomonas aeruginosa KX828570 65.9% > Marshland soil+OSD/Seacare+ Pseudomonas aeruginosa KX828570 52.6% > Marshland soil+OSD/Seacare+ Pseudomonas aeruginosa KX828570 47.6%. Dispersant utilizing bacterial count in marshland and terrestrial soil ranged from 6.013 to 7.338 log10Cfu/g and 6.045 to 7.301 log10Cfu/g respectively from Day 1 to 28. Conclusion: From the investigation, it revealed that Oil spill dispersants are more degradable in terrestrial soil than marshland soil. OSD/Seacare is more biodegradable than OSD/LT in both terrestrial and marshland soil augmented with Pseudomonas aeruginosa KX828570. Thus, Pseudomonas aeruginosa KX828570 have been found to be a potential bioremediation agent in oil spill dispersant polluted marshland and terrestrial soil.

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Section: Source Of Microorganisms (Pseudomonas Aeruginosa)mentioning

confidence: 99%

“…The plates were incubated at 37°C for 24 hr. Discrete colonies on the plates were aseptically transferred into agar slants and bijou bottles containing 10% (v//v) glycerol, properly labeled and stored as stock cultures for preservation and identification [22,23].…”

Section: Isolation Of Test Organismmentioning

confidence: 99%

Bioremediation Potential of Pseudomonas aeruginosa KX828570 on Crude Oil Spill Polluted Marshland and Terrestrial Soil Treated with Oil Spill Dispersant

Nrior

Inweregbu

2019

JAMB

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“…Impact of the pollution is dependent on the volume of the hydrocarbons and the impact it has on the environment [35,36]. temperature, the availability of oxygen, ph, moisture content, the kind of microbe, the available nutrients and the type of soil are the conditions which control the breakdown of crude oil pollutants [37,38,39,40]. Hence, the essence for this study is to evaluate and compare the bioremediation potential of Pseudomonas aeruginosa and Bacillus megaterium on oil spill dispersant polluted terrestrial soil.…”

Section: Introductionmentioning

confidence: 99%

Comparative Bioremediation Potentials of Pseudomonas aeruginosa KX828570 and Bacillus megaterium KY085976 on Polluted Terrestrial Soil Treated with Oil Spill dispersant

Williams

Inweregbu

2019

SAJRM

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Aim: To assess the bioremediation potential of Pseudomonas aeruginosa and Bacillus megaterium on oil spill dispersant polluted terrestrial soil. Study Design: The study employs experimental design, statistical analysis of data and interpretation. Place and Duration of the Study: Polluted Terrestrial Soil (Ts) samples were collected from Kegbara-Dere community in Gokana Local Government Area of Rivers State with sterile shovel from three different spots at the same location and put in black polythene bags and transported to the microbiological laboratory within 24 hours for physicochemical and microbiological analyses. Oil spill dispersant (OSD/LT and OSD/Seacare) were obtained from Baker and Hughes Nigeria Limited in Rivers state, Nigeria. Methodology: Standard microbiological procedures were used to enumerate, isolate and identify the bacterial isolates. Pseudomonas aeruginosa and Bacillus megaterium in oil spill dispersants contaminated soil were monitored over a period of 1, 7, 14, 21 and 28 days respectively for their bioremediation potentials. Results: The presence of Pseudomonas aeruginosa (Pa) or Bacillus megaterium(Bm) in oil spill dispersant polluted soils enhanced decrease in Total Hydrocarbon Content (THC) of the soil. THC for control soil reduced from 18348.68 mg/kg to 9111.84 mg/kg; TS+OSD/LT+Bm, 18348.68 to 7092.11 mg/kg; TS+OSD/LT+Pa, 18348.68 to 6263.16(mg/kg); TS+OSD/LT+Bm+Pa, 18348.68 to 2473.68 mg/kg; TS+OSD/SC+Bm, 18348.68 to 6421.05 mg/kg; TS+OSD/SC+Pa, 18348.68 to 5618.42 mg/kg; TS+OSD/SC+Bm+Pa, 18348.68 to 5835.53 mg/kg, between the first (day 1 ) and last day ( day 28). The percentage (%) bioremediation rate of polluted soil was as follows: control (TS(CRTL) 50.3%, TS+OSD/LT+Bm 61.3%, OSD/LT+Pa 65.9%, and OSD/LT+Bm+Pa 86.5% Whereas, TS+OSD/Seacare+Bm had 65.0%, OSD/Seacare+Pa 69.4%, OSD/Seacare+Bm+Pa 68.2% respectively. The highest percentages of THC in this study were from soil samples treated with oil spill dispersant and organisms while the least was observed in treatments without oil spill dispersant and organism. This suggests that microorganisms are more abundant in oil spill dispersant polluted soils than unpolluted soils. Conclusion: From this study, it was observed that bioremediation of dispersant polluted environments could be achieved by stimulation of native microorganisms such as Pseudomonas aeruginosa KX828570 and Bacillus megaterium KY085976 and this would be cost effective in the clean-up strategy for such pollutants.

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AN ASSESSMENT OF THE PERFORMANCE OF MAIZE (Zea Mays L.) AS AFFECTED BY PALM OIL MILL EFFLUENTS IN ABRAKA, DELTA STATE, NIGERIA

Enujeke,

Umukoro,

Imade

et al. 2023

FJS

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The study was carried out to investigate the possible toxic effect of palm oil mill effluents on maize at varying concentrations. 2kg of soil samples obtained from site II, Delta State University, Abraka was contaminated with different concentrations (25, 50, 75 and 100%) of palm oil processing effluent alongside the control which served as guide for the determination of the toxicity of palm oil processing effluents. The results showed that maize seeds sown in the control soil had 100% germination with germination sighted at the 4th day after planting. 100% germination rate was also recorded in 25% palm oil effluent contaminated soils with germination commencing at the 4th day after planting. 50% concentration showed 55.6% germination rate starting at 6 days after planting. 75% concentration showed 27.6% germination rate starting at day 6 after planting. 100% concentration showed no growth with 0% germination. The highest plant height with mean value of 4.54cm was recorded in the control. The least value of height across the different concentration was observed in the 75% soil contaminated with palm oil mill effluents with plant height of 1.91cm. Seeds sown in 100% POME recorded no growth. The highest leaf number with mean value of 1.71 was recorded in the control. The study showed that pH varied among the different concentration of palm oil mill effluent with a range of 4.06 to 4.34. The study showed that palm oil processing effluents have negative effects on the germination and growth of maize.

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Assessment of the Physicochemical and Microbiological Quality of Palm Oil Mill Effluent (POME) and Soil in Aluu, Rivers State, Nigeria

Cited by 3 publications

References 0 publications

Bioremediation Potential of Pseudomonas aeruginosa KX828570 on Crude Oil Spill Polluted Marshland and Terrestrial Soil Treated with Oil Spill Dispersant

Bioremediation Potential of Pseudomonas aeruginosa KX828570 on Crude Oil Spill Polluted Marshland and Terrestrial Soil Treated with Oil Spill Dispersant

Comparative Bioremediation Potentials of Pseudomonas aeruginosa KX828570 and Bacillus megaterium KY085976 on Polluted Terrestrial Soil Treated with Oil Spill dispersant

AN ASSESSMENT OF THE PERFORMANCE OF MAIZE (Zea Mays L.) AS AFFECTED BY PALM OIL MILL EFFLUENTS IN ABRAKA, DELTA STATE, NIGERIA

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