Assessment of the potential for mixed-function oxygenase enzyme induction in the extrahepatic tissues of cunners (tautogolabrus adspersus) during reproduction

A preliminary investigation into the use of biomarkers for the monitoring of an ocean outfall

“…Exposure periods in experimental studies vary. While Martel et al (1994) and Porter et al (1989) chose 96 and 90 h exposures, respectively, induction has been shown clearly to occur in as little as 24 h (Bowman and Rand, 1980;Kleinsmith and Kish, 1988). The exposure period of 72 h used in this study was selected to be within this range and to reflect what might reasonably be considered to be about that for which fish might be continuously exposed in the field.…”

“…In the first phase of detoxication, a cytochrome P450 mixed function oxidase (MFO) enzyme system inserts an atom of oxygen into the organic molecule, thereby producing a more water-soluble product. Increases in the activities of MFOs, as measured by ethoxyresorufin-O-deethylase (EROD) and aryl hydrocarbon hydroxylase (AHH) activities, have been used in fish to monitor exposure to chemical contaminants (Andersson et al, 1988;Lindstrom-Seppa and Oikari, 1989;Porter et al, 1989;Holdway et al, 1994;Martel et al, 1994).…”

Effect of Sediment from Two Sulphite-Bleaching Paper Mills on Winter Flounder ( Pleuronectes americanus ) Following Chronic Exposure

“…Sections of spleen and kidney were stained also with Perl's Prussian blue for hemosiderin deposits which were estimated by digital image analysis and expressed as a percentage of the area scanned (Khan and Nag 1993). EROD was assayed fluorometrically in only one group of flounder as reported by Porter et al (1989) using a Perkin-Elmer LS-5 fluorescence spectrophotometer.…”

Iron Ore Mines Leachate Potential for Oxyradical Production

“…Note. PROD activity was measured according to Porter et al (1989) with no preincubation with iron ore prior to adding the substrate. P420 was observed for control rats and PB-induced rats with 0.8% iron ore but was not accompanied by a decrease of cytochrome P450 (Table 7).…”

“…An increases of Note. EROD activity was measured according to Porter et al (1989) with no preincubation with iron ore prior to adding the substrate. Note.…”