Assessment of the protective role of vitamin E in atrazine toxicity on the testis of adult albino rats

Abstract

the positive control group, which received 1 ml of corn oil/day, orally; the Vit E group, which received Vit E at 100 mg/kg/day, orally; the ATZ group, which received ATZ at 300 mg/kg/day, orally; and the ATZ+Vit E group, which received both ATZ and Vit E at the previously mentioned doses. Treatments were given for 6 days/week for 45 days. At the end of the experiment, blood samples were taken to measure serum testosterone level. Semen analysis and estimation of oxidative stress markers catalase, superoxide dismutase, and tissue malondialdehyde were carried out. The testes were processed for light and electron microscopic examination. An immunohistochemical study was performed for detection of Bcl2. ResultsATZ caused a decrease in serum testosterone level and in superoxide dismutase and catalase enzyme activities, whereas malondialdehyde content increased. There was also a decrease in sperm count, viability, and motility in comparison with the control groups. Light microscopic examination of seminiferous tubules revealed degeneration of the germinal epithelium. The lumen contained sloughed cells and homogenous acidophilic material. Ultrastructurally, there was separation of the germinal epithelial cells with small dense nuclei and phagocytic remnants. Sertoli and Leydig cells were also affected. Bcl-2 immunolocalization revealed weak reaction in the cytoplasm of the germinal epithelial cells and Leydig cells. Concomitant use of Vit E caused partial improvement. Conclusion ATZ induced detrimental effects in the testicular tissue that were attenuated by concomitant administration of vitamin E.

The possible protective and therapeutic effects of ginger and cinnamon on the testis and coda epididymis of streptozotocin-induced-diabetic rats: Histological and biochemical studies

“…The procedure was performed using H&E-stained and Ki67 stained sections. Measurements were taken in 10 non overlapping fields for each of four randomly chosen rats from each group, as indicated by Elkhateeb et al (2014) .…”