Assessment of the Sensitivity and Specificity of Enzyme‐Linked Immunosorbent Assay (ELISA) for the Detection of Mycobacterial Antibodies in Bovine Tuberculosis

Ritacco, Viviana; Kantor, Isabel N. de; Barrera, Lucía; Nader, A.; Bernardelli, Amelia; Torrea, Gabriela; Errico, F.; Fliess, Enrique

doi:10.1111/j.1439-0450.1987.tb00377.x

Cited by 30 publications

(25 citation statements)

References 8 publications

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“…In our previous assessment of an enzyme-linked immunosorbent assay (ELISA) for the detection of bovine circulating IgG mycobacterial antibodies, 90. were observed in 89.8 '%, healthy cattle (44/49) from a tuberculosis free area ( RITACCO et al, 1987). Such sensitivity and specificity, which are at least comparable to those attributed to the tuberculin skin test ( FRANCIS et al, 1978), encouraged us to explore further the applicability of this method to the diagnosis of bovine tuberculosis.…”

Section: Introductionmentioning

confidence: 96%

Further Evaluation of an Indirect Enzyme‐Linked Immunosorbent Assay for the Diagnosis of Bovine Tuberculosis

Ritacco¹,

López

Barrera

et al. 1990

Journal of Veterinary Medicine, Series B

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Summary The sensitivity and specificity of an ELISA for the detection of bovine IgG anti‐Mycobacterium bovis antibodies were 73.6% and 94.1%, respectively, as determined in 53 bacteriologically confirmed tuberculous cattle and 101 healthy cattle from a tuberculosis‐free area. In addition, the results of ELISA and tuberculin tests in 149 cattle were compared with those of subsequent necropsy studies. Both tests failed to detect 2 animals with tuberculous lesions and positive culture; 3/12 cattle with M. bovis isolation and no lesions, and 2/7 with atypical mycobacterial infection reacted to tuberculin, but none had antibodies; in 128 cattle with neither lesions nor mycobacterial isolation, 6 were tuberculin reactors and 7 others had antibodies. Negative results were obtained by ELISA in 21/22 paratuberculous cattle. Antibodies were not detected in 88.9 % to 96.4 % of 697 cattle from two tuberculin negative herds of an endemic area. In a herd with proved M. bovis infection, distribution of seropositive animals in tuberculin and non‐tuberculin reactors was similar. Antibody responses to cutaneous tuberculin stimuli were observed in 4 experimentally infected cattle, but only in 2/10 healthy controls after repeated PPD stimuli. Nine controls which had either received a single tuberculin dose or none showed no increase in antibody levels. The low sensitivity of this ELISA limits its usefulness as a diagnostic tool for bovine tuberculosis eradication campaigns. However, it could be helpful in epidemiological surveillance if its efficiency to identify infected herds is demonstrated. Zusammenfassung Weitere Erkenntnisse über einen indirekten ELISA zur Diagnose der bovinen Tuberkulose Zur Prüfung der Sensitivität und Spezifität eines ELISA zur Diagnose boviner Tuberkulose wurden Seren von Rindern mit bakteriologisch gesicherter Tuberkulose (n: 53), sowie von Tieren aus einem tuberkulosefreien Gebiet (n: 101) untersucht; die Resultate stimmten in 73,6 % bzw. 94,1 % der Fälle überein. Des weiteren wurden die ELISA‐ und Tuberkulintest‐Ergebnisse von 149 Rindern mit denen einer nachfolgenden pathologisch‐anatomischen Untersuchung verglichen. Beide Testsysteme waren nicht in der Lage, zwei Tiere mit pathologisch/anatomisch und bakteriologisch gesicherter Tuberkulose zu erkennen; drei von 12 Tieren, aus denen M. bovis isoliert wurde und die keine morphologischen Veränderungen aufwiesen, sowie 2 von 7 Tieren, die mit atypischen Mykobakterien infiziert waren, zeigten zwar eine positive Tuberkulinprobe, aber es konnten keine Antikörper nachgewiesen werden; von 128 Rindern, bei denen weder pathologisch‐anatomische Veränderungen beobachtet werden konnten noch Mycobakterien isoliert wurden, zeigten 6 eine positive Tuberkulinreaktion und 7 weitere Tiere wiesen Antikörper auf. Bei 21 von 22 an Paratuberkulose erkrankten Rindern verliefen die ELISA‐Untersuchungen negativ. Bei Rindern aus zwei Tuberkulin‐negativen Herden (n: 697) konnten ebenfalls in 88,9% und 96,4% der Fälle keine Antikörper festgestellt werden. In einer Rinderherde mit ...

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Section: Introductionmentioning

confidence: 96%

Further Evaluation of an Indirect Enzyme‐Linked Immunosorbent Assay for the Diagnosis of Bovine Tuberculosis

Ritacco¹,

López

Barrera

et al. 1990

Journal of Veterinary Medicine, Series B

Self Cite

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“…However, some infected animals may have an antibody response in the absence of cell‐mediated responses, particularly when the bacterial load is high [4]. A number of enzyme‐linked immunosorbant assay (ELISA) tests have been described based on complex M. bovis antigens, such as purified protein derivative (PPD) [5–8] and phosphatide antigens [9]. All of these assays were successful in detecting circulating antibodies to mycobacteria but have been considered to lack specificity.…”

Section: Introductionmentioning

confidence: 99%

Characterization of the Early Antibody Response in Bovine Tuberculosis: MPB83 is an Early Target with Diagnostic Potential

et al. 2001

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A 26-kDa antigen has been shown to be a dominant antibody target in Mycobacterium bovis-infected cattle. In this study, that antigen was used as an immunogen to raise a panel of mouse monoclonal antibodies. The majority of those bound to native protein with a molecular mass of 26 kDa and to recombinant MPB83, strongly suggesting that MPB83 is an important B-cell antigenic target in bovine tuberculosis. In order to provide assessment of the potential of measuring antibody responses to the native protein, one monoclonal antibody, 1F11, was incorporated into an enzyme-linked immunosorbant assay format to trap antigen from a crude bacterial extract. Despite some disadvantages of this format, serum samples from cattle which had been infected experimentally with M. bovis, and from tuberculin skin-test-negative and -positive field cattle were tested for the presence of antibodies. Data from the skin-test-negative cattle allowed an arbitrary cut-off value to be established and, under these conditions, test sensitivity and specificity were estimated at 37.5 and 89%, respectively. These results indicate potential for MPB83 in the development of assays for serological diagnosis of bovine tuberculosis.

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“…However, these have usually lacked adequate specificity, perhaps due to the use of complex antigens such as M. bovis soni-cate or PPD (Auer 1987;Ritacco et al 1987;Hanna et al 1989) or have poor sensitivity when defined antigens such as MPB70 have been employed (Fifis et al 1989;Harboe et al 1990). However, these have usually lacked adequate specificity, perhaps due to the use of complex antigens such as M. bovis soni-cate or PPD (Auer 1987;Ritacco et al 1987;Hanna et al 1989) or have poor sensitivity when defined antigens such as MPB70 have been employed (Fifis et al 1989;Harboe et al 1990).…”

Section: Tuberculin Skin Testingmentioning

confidence: 99%

Immunodiagnosis of Mycobacterial Infection

et al. 1999

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Assessment of the Sensitivity and Specificity of Enzyme‐Linked Immunosorbent Assay (ELISA) for the Detection of Mycobacterial Antibodies in Bovine Tuberculosis

Cited by 30 publications

References 8 publications

Further Evaluation of an Indirect Enzyme‐Linked Immunosorbent Assay for the Diagnosis of Bovine Tuberculosis

Further Evaluation of an Indirect Enzyme‐Linked Immunosorbent Assay for the Diagnosis of Bovine Tuberculosis

Characterization of the Early Antibody Response in Bovine Tuberculosis: MPB83 is an Early Target with Diagnostic Potential

Immunodiagnosis of Mycobacterial Infection

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