Assessment of the Substance Antioxidative Profile by Hyaluronan, Cu(II) and Ascorbate

Valachová, Katarína; Šoltés, Ladislav

doi:10.3390/pharmaceutics13111815

Cited by 4 publications

(4 citation statements)

References 76 publications

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“…The scavenging activity of the hydrogel against reactive oxygen species (ROS) was assessed using an OH· scavenging assay, and the results were similar to those of the reactive nitrogen species (RNS) scavenging assay. [ 26 ]…”

Section: Resultsmentioning

confidence: 99%

“…The scavenging activity of the hydrogel against reactive oxygen species (ROS) was assessed using an OH• scavenging assay, and the results were similar to those of the reactive nitrogen species (RNS) scavenging assay. [26] The biocompatibility of the HPADN hydrogels was evaluated in vitro. NCTC clone 929 (L929) cells were co-incubated with hydrogels and the hydrogels were placed in Transwell, and live/dead staining was performed.…”

Section: Preparation and Characterization Of Hydrogelsmentioning

confidence: 99%

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Glycopeptide‐Based Multifunctional Hydrogels Promote Diabetic Wound Healing through pH Regulation of Microenvironment

Xia

Dong

Tang

et al. 2023

Adv Funct Materials

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Excessive inflammation, bacterial infection, and blocked angiogenesis make diabetic wound healing challenging. Multifunctional wound dressings have several advantages in diabetic wound healing. In addition, the pH regulation of the microenvironment is shown to be a key factor that promotes skin regeneration through cellular immune regulation. However, few reports have focused on the development of functional dressings with the ability to regulate the pH microenvironment and promote diabetic wound healing. This study presents a novel approach for regulating the pH microenvironment of diabetic wound sites using a glycopeptide-based hydrogel consisting of modified hyaluronic acid and poly(6-aminocaproic acid). This hydrogel forms a network through Schiff base interactions and metal complexation, which suppresses inflammation and accelerates angiogenesis during wound healing. Hydrogels not only have adequate mechanical properties and self-healing ability but can also support tissue adhesion. They can also promote the secretion of inducible cAMP early repressor, which promotes the polarization of macrophages toward the M2 type. The in vivo results confirm that hydrogel promotes diabetic wound repair and skin regeneration by exerting rapid antiinflammatory effects and promoting angiogenesis. Therefore, this hydrogel system represents an effective strategy for treating diabetic wounds.

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Section: Resultsmentioning

confidence: 99%

Section: Preparation and Characterization Of Hydrogelsmentioning

confidence: 99%

Glycopeptide‐Based Multifunctional Hydrogels Promote Diabetic Wound Healing through pH Regulation of Microenvironment

Xia

Dong

Tang

et al. 2023

Adv Funct Materials

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“…In a simple in vitro assay, we have shown for the first time that the addition of 2% ADM to 1% high molecular weight HA solution inhibits free radical-induced degradation of HA, as properly monitored by changes in dynamic viscosity [48]. Decrease of HA viscosity is, in fact, known to reflect HA degradation [49].…”

Section: Discussionmentioning

confidence: 99%

Adelmidrol Protects Hyaluronic Acid against Oxidative Degradation and Improves the Outcome in Patients with Adhesive Capsulitis of the Shoulder Managed by Physical Therapy. In vitro Evidence and Two Case Reports

2024

J Orthop Res Ther

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Adhesive capsulitis (AC) or frozen shoulder is an inflammatory condition characterized by joint stiffness and pain. Although its pathogenesis is not fully elucidated, inflammation (either acute or low-grade inflammation) is believed to play a crucial role. An increase in reactive oxygen and nitrogen species also occurs in AC. The management of AC is challenging for orthopedic surgeons and physical therapists. Intra-articular hyaluronate injection is a treatment option. Yet, failure is reported and may depend upon oxidative breakdown of hyaluronan. Adelmidrol is a palmitoylethanolamide analogue and azelaic acid derivative exerting antioxidant activity in a number of fibrotic disorders. Here we first report the in vitro ability of Adelmidrol to protect hyaluronan against oxidative degradation and to exert an antioxidant effect against hydrogen peroxide-induced increase in nitrite release and intracellular reactive oxygen species in the macrophage J774A.1 cell line. We then investigated a new intra-articular formulation of high molecular weight (1300-2000 kDa) hyaluronic acid (1%) and Adelmidrol (2%) in two AC patients partially responding to physical therapy. Within a tailored physical therapy approach, three fortnightly intra-articular injections of this formulation allowed for a progressive reduction in pain severity, as well as improved range of motion and daily living activities, with the good outcome being maintained at the six-month follow-up in both patients. Overall, the data show Adelmidrol's protective activity against hyaluronic acid oxidation and provide preliminary information for further studies to investigate the association of Adelmidrol and high molecular weight hyaluronic acid in the management of AC patients.

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“…The linear poly-anion, structurally formed by a repeating disaccharide structure [(1→3)-β-d-GlcNAc-(1→4)-β-d-GlcA-] [ 62 ] that is the HA, was first isolated from the vitreous humor of cow’s eyes. The HA is also naturally present in the extracellular matrix of connective tissue, skin, vitreous humor, synovial fluid, and many other tissues and organs of the body, accounting for 15 g in the case of a 70 kg adult [ 63 ]. Starting from the studies of Pagnacco et al [ 64 ] who assessed the antibacterial, anti-edematous, and anti-inflammatory effects of HA when used as a topical treatment for periodontitis in a clinical trial, we decided to use HA as a matrix for antibiotics and MEL.…”

Section: Introductionmentioning

confidence: 99%

Considerations on the Controlled Delivery of Bioactive Compounds through Hyaluronic Acid Membrane

et al. 2022

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(1) Background: The standard treatment for periodontal disease, a chronic inflammatory state caused by the interaction between biofilms generated by organized oral bacteria and the local host defense response, consists of calculus and biofilm removal through mechanical debridement, associated with antimicrobial therapy that could be delivered either systemically or locally. The present study aimed to determine the effectiveness of a hyaluronic acid membrane matrix as a carrier for the controlled release of the active compounds of a formulation proposed as a topical treatment for periodontal disease, and the influence of pH on the complex system’s stability. (2) Methods: The obtained hyaluronic acid (HA) hydrogel membrane with dispersed melatonin (MEL), metronidazole (MZ), and tetracycline (T) was completely characterized through FTIR, XRD, thermal analysis, UV-Vis and fluorescence spectroscopy, fluorescence microscopy, zeta potential and dielectric analysis. The MTT viability test was applied to check the cytotoxicity of the obtained membranes, while the microbiological assessment was performed against strains of Staphylococcus spp. and Streptococcus spp. The spectrophotometric investigations allowed to follow up the release profile from the HA matrix for MEL, MZ, and T present in the topical treatment considered. We studied the behavior of the active compounds against the pH of the generated environment, and the release profile of the bioactive formulation based on the specific comportment towards pH variation. The controlled delivery of the bioactive compounds using HA as a supportive matrix was modeled applying Korsmeyer–Peppas, Higuchi, first-order kinetic models, and a newly proposed pseudo-first-order kinetic model. (3) Results: It was observed that MZ and T were released at higher active concentrations than MEL when the pH was increased from 6.75, specific for patients with periodontitis, to a pH of 7.10, characterizing the healthy patients. Additionally, it was shown that for MZ, there is a burst delivery up to 2.40 × 10−5 mol/L followed by a release decrease, while for MEL and T a short release plateau was recorded up to a concentration of 1.80 × 10−5 mol/L for MEL and 0.90 × 10−5 mol/L for T, followed by a continuous release; (4) Conclusions: The results are encouraging for the usage of the HA membrane matrix as releasing vehicle for the active components of the proposed topical treatment at a physiological pH.

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Assessment of the Substance Antioxidative Profile by Hyaluronan, Cu(II) and Ascorbate

Cited by 4 publications

References 76 publications

Glycopeptide‐Based Multifunctional Hydrogels Promote Diabetic Wound Healing through pH Regulation of Microenvironment

Glycopeptide‐Based Multifunctional Hydrogels Promote Diabetic Wound Healing through pH Regulation of Microenvironment

Adelmidrol Protects Hyaluronic Acid against Oxidative Degradation and Improves the Outcome in Patients with Adhesive Capsulitis of the Shoulder Managed by Physical Therapy. In vitro Evidence and Two Case Reports

Considerations on the Controlled Delivery of Bioactive Compounds through Hyaluronic Acid Membrane

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