Assessment of the Toxic Potential of Sewage Sludge in the Midgut of the Diplopod Rhinocricus padbergi

Perez, Danielli Giuliano; Fontanetti, Carmem Silvia

doi:10.1007/s11270-010-0657-9

Cited by 18 publications

(3 citation statements)

References 19 publications

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“…The hepatic cells actively participates in detoxification process of the organism. (Kohler, 2002;Fontanetti, 2010, 2011;Perez and Fontanetti, 2011;Merlini et al, 2012). These observations explain the fact that large quantities of spherocrystals are found in the cytoplasm of these cells in millipede U. atrobrunneus.…”

Section: Discussionmentioning

confidence: 92%

Midgut of the diplopod Urostreptus atrobrunneus: structure, function, and redefinition of hepatic cells

2017

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Diplopods are considered important macroarthropods the soil as part of its maintenance and balance. These animals usually do not occur in high densities, but population explosions caused by environmental disturbances, climate changes, and use of pesticides that eliminate possible competitors, have been reported. The millipede Urostreptus atrobrunneus Pierozzi and Fontanetti, 2006 have become a nuisance to humans in infestation sites in urban centers of the state of Sao Paulo, Brazil. As a contribution to the understanding of this potential pest, this study describes the histology, histochemistry, and ultrastructure of the U. atrobrunneus midgut, and presents the redefinition of hepatic cells somewhat controversial in the literature. The region of the midgut is characterized by the absence of a cuticular intima, and composed of a pseudostratified epithelium on a thick basal membrane, followed by a muscle layer, a layer of hepatic cells, lined by an external membrane. The morphology observed in U. atrobrunneus is similar to that reported for other species of diplopods. The hepatic cells have been previously described as randomly without forming a layer, however, the present results clearly demonstrate that these cells form a continuous layer over the whole midgut.Keywords: histology, histochemistry, ultrastructure, Diplopoda, digestive tract. Intestino médio do diplópodo Urostreptus atrobrunneus: estrutura, função e redefinição das células hepáticas ResumoDiplópodos são considerados importantes macro-artrópodes do solo, uma vez que participam de sua manutenção e equilíbrio. Comumente estes animais não apresentam população numerosa, porém há relatos de explosões populacionais ocasionadas por desequilíbrios ambientais, mudanças climáticas e utilização de pesticidas que eliminam possíveis competidores. O milípede Urostreptus atrobrunneus Pierozzi e Fontanetti, 2006, têm apresentado pontos de infestação em centros urbanos, do estado de São Paulo, Brasil, causando muitos transtornos à população humana. Com objetivo de contribuir para o conhecimento desta potencial praga, este trabalho apresenta a descrição histológica, histoquímica e ultra-estrutural do intestino médio do milípede U. atrobrunneus, bem como apresenta a redefinição das células hepáticas, um tanto controversa na literatura pertinente. A região do intestino médio é caracterizada pela ausência da íntima cuticular, sendo formado por um epitélio pseudoestratificado, apoiado por uma membrana basal espessa, seguido de uma camada muscular, uma camada de células hepáticas, revestido por uma membrana externa. A morfologia observada neste trabalho assemelha-se bastante com as descrições de outras espécies de diplópodos estudadas até o momento. As células hepáticas foram previamente descritas como dispostas aleatoriamente sem a formação de uma camada, contudo, os presentes resultados demonstram claramente que estas células formam uma camada contínua ao longo de todo o intestino médio.

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Section: Discussionmentioning

confidence: 92%

Midgut of the diplopod Urostreptus atrobrunneus: structure, function, and redefinition of hepatic cells

2017

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“…In millipedes, hemocytes have been described as accumulating among cells of the fat body that surrounds the digestive system in animals treated with heavy metals. The number of hemocytes increased when a number of degenerated cells of the fat body occurred (de Godoy & Fontanetti 2010;Perez & Fontanetti 2011). The increase in circulating hemocyte number caused by heavy metals has been described for some arthropods (Lorenzon et al 2001;Stalmach et al 2015).…”

Section: Groupsmentioning

confidence: 94%

“…It is connected with the fact that myriapods, feeding on detritus, leaves, plant and animal organic matter or other animals, take part in soil mineralization and its decomposition. Studies on this arthropod group have focused on different organs and tissues in which the toxic substances (e.g., heavy metals) could be accumulated (Köhler & Alberti 1992;Köhler et al 1995;Vandenbulcke et al 1998a;Köhler 2002;Perez & Fontanetti 2011;Souza & Fontanetti 2011).…”

Section: Introductionmentioning

confidence: 99%

Influence of soil contaminated with cadmium on cell death in the digestive epithelium of soil centipedeLithobius forficatus(Myriapoda, Chilopoda)

Rost‐Roszkowska

Poprawa

Chajec

et al. 2020

The European Zoological Journal

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Cadmium is a heavy metal that is treated as an environmental pollutant (air, water, soil). In order to understand the potential effects of cadmium in soil and soil invertebrates, it is important to describe all alterations which appear at different levels in organisms. The main aim of this study was to investigate, analyze and describe the alterations caused by cadmium short-and long-term intoxication at different levels in the organisms: from tissues to cells and organelles. In addition, the activation of cell death mechanisms that take part in homeostasis maintenance according to cadmium has been studied. Therefore, as the species for this project, a terrestrial and well-known widespread European speciesthe centipede Lithobius forficatus (Myriapoda, Chilopoda, Lithobiomorpha)was chosen. This omnivorous species lives under upper layers of soil, under stones, litter, rocks, and leaves, and it is also commonly found in human habitats. The animals were divided into three groups: Cthe control group, animals cultured in a horticultural soil; Cd1animals cultured in a horticultural soil supplemented with 80 mg/kg (dry weight) of CdCl 2 , 12 daysshort-term exposure; Cd2animals cultured in a horticultural soil supplemented with 80 mg/kg (dry weight) of CdCl 2 , 45 dayslong-term exposure. The midgut was isolated from each specimen and it was prepared for analysis using some histological, histochemical and immunohistochemical methods. Our studies showed that short-term intoxication causes intensification of autophagy and digestion of reserve material, while long-term exposure to this heavy metal causes activation of cell death processes together with inhibition of autophagy connected with the lack of reserve material. Additionally, we can infer that autophagy and cell death are nutrient deprivation-induced processes. Finally, we can conclude that short-and long-term exposure of soil centipede to cadmium affects different mechanisms and processes of cell death.

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Toxicity of trifluralin herbicide in a representative of the edaphic fauna: Histopathology of the midgut of Rhinocricus padbergi (Diplopoda)

Merlini

Nogarol

Marin-Morales

et al. 2012

Microscopy Res & Technique

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The aim of this study was to use the diplopod Rhinocricus padbergi as test organism to verify the possible toxic effects of the herbicide trifluralin in a representative of the edaphic fauna. Histological and histochemical techniques were applied in the midgut of the animals after exposure to different concentrations of trifluralin for 7 and 90 days (acute and subchronic exposure, respectively). The individuals from the control group did not present alterations to any of the exposures. In the acute exposure, the group exposed to a quarter and half of the field dose presented an accumulation of cytoplasmic granules in the hepatic cells; the field dose group presented an increase in the rate of epithelial renewal and the group exposed to double the field concentration presented an increase in the release of secretory vesicles. The subchronic response was obtained with the field dose and double field dose because the animals from a quarter and half groups did not survive until the end of the experiment. In both groups, the animals presented disruption of the epithelium and higher occurrence of hemocytes among the hepatic cells. The group exposed to double the field dose presented sites of cytoplasmic vacuolization, dilatation of the intercellular space, increase in the epithelial renewal, and release of secretory vesicles. The results alert for a careful use of this herbicide, since it promoted alterations in the midgut cells of the test organism used.

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Assessment of the Toxic Potential of Sewage Sludge in the Midgut of the Diplopod Rhinocricus padbergi

Cited by 18 publications

References 19 publications

Midgut of the diplopod Urostreptus atrobrunneus: structure, function, and redefinition of hepatic cells

Midgut of the diplopod Urostreptus atrobrunneus: structure, function, and redefinition of hepatic cells

Influence of soil contaminated with cadmium on cell death in the digestive epithelium of soil centipedeLithobius forficatus(Myriapoda, Chilopoda)

Toxicity of trifluralin herbicide in a representative of the edaphic fauna: Histopathology of the midgut of Rhinocricus padbergi (Diplopoda)

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