Assessment of the Viability of NIH3T3 Fibroblast Cells Cultured in Polymer Matrices with rhGH

Gerzson, Alexandre da Silveira; Silva, Rafaela Radomski da; Valente, Cristhiane Alvim; Pagnoncelli, Rogério Miranda

doi:10.1007/s10924-017-0984-7

Cited by 3 publications

(6 citation statements)

References 24 publications

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“…Several polymers, copolymers, and blends are used to produce biomaterials such as polycaprolactone (PCL) and poly(lactic‐ co ‐glycolic acid) (PLGA). These are biodegradable polymers approved by the Food and Drug Administration (FDA) for clinical use . PLGA is one of the most widely used biodegradable polymers in scaffold preparation for tissue engineering owing to its easy manufacturing, low inflammatory response and adjustability of degradation rates .…”

Section: Introductionmentioning

confidence: 99%

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Design and optimization of biocompatible polycaprolactone/poly (l‐lactic‐co‐glycolic acid) scaffolds with and without microgrooves for tissue engineering applications

Valente

Chagastelles

Nicoletti

et al. 2018

J Biomedical Materials Res

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This study investigated the effects of smooth and microgrooved membrane blends, with different polycaprolactone (PCL)/ poly(lactic-co-glycolic acid) (PLGA) ratios on the viability, proliferation, and adhesion of different mammalian cell types. The polymer matrices with and without microgrooves, obtained by solvent casting, were characterized by field-emission scanning electron microscopy, atomic force microscopy, contact angle and Young's modulus. Blend characterization showed an increase in roughness and stiffness of membranes with 30% PLGA, without any effect on the contact angle value. Pure PCL significantly decreased the viability of Vero, HaCaT, RAW 264.7, and human fetal lung and gingival fibroblast cells, whereas addition of increasing concentrations of PLGA led to a reduced cytotoxicity. Increased proliferation rates were observed for all cell lines. Fibroblasts adhered efficiently to smooth membranes of the PCL70/PLGA30 blend and pure PLGA, compared to pure PCL and silicone. Microgrooved membranes promoted similar cell adhesion for all groups. Microstructured membranes (15 and 20-μm wide grooves) promoted suitable orientation of fibroblasts in both PCL70/PLGA30 and pure PLGA, as compared to pure PCL. Neuronal cells of the dorsal root ganglion exhibited an oriented adhesion to all the tested microgrooved membranes. Data suggest a satisfactory safety profile for the microgrooved PCL70/PLGA30 blend, pointing out this polymer combination as a promising biomaterial for peripheral nerve regeneration when cell orientation is required. © 2018 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 106A: 1522-1534, 2018.

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Section: Introductionmentioning

confidence: 99%

“…PLGA‐based biomaterials with nano/microporous allow nutrient permeability and favor cell adhesion and proliferation . PCL presents good mechanical proprieties and has been used in blends with other biocompatible polymers to counterbalance biological and mechanical features .…”

Section: Introductionmentioning

confidence: 99%

Design and optimization of biocompatible polycaprolactone/poly (l‐lactic‐co‐glycolic acid) scaffolds with and without microgrooves for tissue engineering applications

Valente

Chagastelles

Nicoletti

et al. 2018

J Biomedical Materials Res

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“…Several polymers, copolymers, and biodegradable polymer blends are used to produce and design polymeric biodegradable products for biomedical applications with varied life cycles and structures . Poly(L‐lactic‐co‐glycolic acid) (PLGA) is one such synthetic biodegradable polymer that has been approved by the Food and Drug Administration (FDA) for used in therapeutic devices.…”

Section: Introductionmentioning

confidence: 99%

“…Fiber polymeric mats are used in biomedical applications such as wound dressing, tissue engineering, and enzyme immobilization . Their porous structure results in a high surface‐to‐volume ratio that makes them suitable for medical applications .…”

Section: Introductionmentioning

confidence: 99%

Preparation and Characterization of Polymeric Microfibers of PLGA and PLGA/PPy Composite Fabricated by Solution Blow Spinning

Nahuis

Valente

Oliveira

et al. 2019

Macromolecular Symposia

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In this study, microfibers of poly(L‐lactic‐co‐glycolic acid) (PLGA) and PLGA/polypyrrole (PPy) composite (90/10 wt%) are produced by using the solution blow spinning (SBS) technique. PPy is synthesized by the oxidative polymerization method using p‐toluenesulfonic acid (p‐TSA) as the dopant and FeCl3 as the oxidant. The prepared PPy showed microfibers with globular particles morphology. Mixtures of porous and nonporous microfibers and microfibers incorporated with PPy are obtained. A wettability test shows that the PLGA and PLGA/PPy fibrous mats are hydrophobic. The electrical conductivity of the PLGA/PPy composite is of the same order as that of pure PLGA (≈10−10 S cm−1), indicating that the electrical percolation threshold is not reached for PPy loading of 10 wt%. The incorporation of PPy into PLGA microfibers improved the thermal stability of the composite and also increases the PLGA crystalline phase.

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“…This process of reabsorption of the alveolar ridge follows progressively throughout life, and it may be impossible to rehabilitate implants, since this therapy is based on the principle of correct three-dimensional positioning of the implant in the socket bone. [1] A wide range of materials and techniques has been proposed for socket preservation. Autogenous bone, biomaterials, and different membranes and more recently the blood concentrates are some examples among the most used for this purpose.…”

Section: Introductionmentioning

confidence: 99%

Socket Preservation using Human Recombinant Growth Hormone Associated with Bio-Oss Biomaterial with the Purpose of Improving Healing and Reducing Treatment Time - A Case Report

Giacomin¹,

Warmeling²,

Silva³

et al. 2019

J Clin Res Dent

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The purpose of this article is to describe a clinical case where recombinant human growth hormone (rhGH) associated with Bio-Oss  was used in socket preservation. 53-year-old female patient, ASA I, attended the dental clinic presenting a root fracture of the second upper right premolar. The treatment plan consisted of tooth removal and socket preservation with rhGH and Bio-Oss. After 4 months, a tomography scan was performed before implant placement. At the time of the implant placement, a bone sample was sent for histological analysis. The collected material was analyzed histologically and demonstrated mature bone, with areas of neoformation and deposition of bone matrix and the tomographic aspect was of a hypodense image inside the socket. Socket preservation realized with rhGH associated with Bio-Oss  was able to maintain the three-dimensional dimensions of the sockets, promoting neoformed bone with histological and radiographic features of maturation and was able to reduce the time of treatment.

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Assessment of the Viability of NIH3T3 Fibroblast Cells Cultured in Polymer Matrices with rhGH

Cited by 3 publications

References 24 publications

Design and optimization of biocompatible polycaprolactone/poly (l‐lactic‐co‐glycolic acid) scaffolds with and without microgrooves for tissue engineering applications

Design and optimization of biocompatible polycaprolactone/poly (l‐lactic‐co‐glycolic acid) scaffolds with and without microgrooves for tissue engineering applications

Preparation and Characterization of Polymeric Microfibers of PLGA and PLGA/PPy Composite Fabricated by Solution Blow Spinning

Socket Preservation using Human Recombinant Growth Hormone Associated with Bio-Oss Biomaterial with the Purpose of Improving Healing and Reducing Treatment Time - A Case Report

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