Assessment of Tobacco Specific Nitrosamines in Tobacco Genotypes

Abstract

The tobacco-specific nitrosamines (TSNAs) are one of the most important groups of carcinoigens in tobacco leaf, knowing the difference of TSNAs contents in tobacco genotypes should be beneficial for breeding low TSNAs cultivars which could decrease the harmfulness to human health in tobacco products. For this purpose, 8 genotypes (include burley, sun-cured and flue-cured tobacco) were selected as materials, the TSNAs contents of uncured and cured leaves were assessed with LC-MS/MS. The results showed that TSNAs contents were significantly increased after curing in all genotypes. The mean TSNAs contents of leaves before and after curing in burley were the highest among three types of tobacco, and the contents in sun-cured tobacco were the lowest. Coefficient of variation (CV) of TSNAs among genotypes in burley was higher than that in flue-cured and sun-cured tobacco, and the CV in sun-cured tobacco was the lowest. The positive correlation was observed in NNN, NAT and total TSNAs contents between uncured and cured leaves in three types of tobacco. Therefore, the TSNAs contents in uncured leaves could be considered as evaluation index to screen tobacco cultivars with the low TSNAs contents.

No-tillage culture and nitrogen fertilizer management for burley tobacco production

“…Tobacco-specific nitrosamines (TSNAs) have been reported as carcinogenic compounds in tobacco for many years (MacKown et al 1984). Since the first report documenting the presence of N-nitrosonornicotine in unburned tobacco and induced malignant tumours in mice, rats and hamsters (Hoffmann & Hecht 1985), there have been many studies evaluating the effects of genotype (Guo et al 2013), chemical applications (Li et al 2005), and the regulation of humidity and temperature during the curing process on TSNAs formation (Burton et al 1989 a ; Chamberlain & Chortyk 1992; Padmavathy et al 2011; De Godoy Lusso et al 2014). The effect of liming, N fertilizer rate, irrigation management and cultivar on reducing sugars, nicotine concentration and leaf quality have also been investigated (Karaivazoglou et al 2007; Çakir & Çebi 2010; Rathbone et al 2010).…”

Production of alkaline pectinase: a case study investigating the use of tobacco stalk with the newly isolated strain Bacillus tequilensis CAS-MEI-2-33

“…tequilensis CAS-MEI-2-33. However, pectinase activity with 50 g/L TS in the fermentation medium was not significantly different from that with 40 g/L TS, and this similarity might be caused by the other components, such as tobacco-specific nitrosamines (TSNAs) ([31] #1310).In the optimized fermentation (40 h, pH 7.0, 40 g/L TS, 3% inoculum), the highest pectinase activity was 1370 U/ml. By optimizing the inoculum amount, pectinase activity was improved 1.88 times.…”