Assessment of uterine luminal pH in mares and the effect of dilute vinegar lavage on uterine luminal pH and endometrial health

Thompson, Renee L.; Gunn, Allan; Stephen, Cyril; Ip, Heather; Brookes, Victoria

doi:10.1016/j.theriogenology.2018.05.014

Cited by 5 publications

(4 citation statements)

References 34 publications

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“…The UvrA protein has been identified and functionally characterized in different species of pathogenic bacteria, including Helicobacter pylori [ 37 , 38 ], Streptococcus mutans [ 39 ], Arcanobacterium hemolyticum [ 40 ], Listeria monocytogenes [ 41 ], Mycobacterium tuberculosis [ 42 ], and Borrelia burgdorferi [ 43 ]. Similarly, the UvrA protein could provide the survival of A. hippocoleae in the upper reproductive tract of mares where it can become acidic, especially during diestrus [ 44 ]. The protein may also protect the bacteria from phagocytic destruction because of exposure to reactive oxygen and nitrogen species.…”

Section: Discussionmentioning

confidence: 99%

Genome Sequence Comparisons between Small and Large Colony Phenotypes of Equine Clinical Isolates of Arcanobacterium hippocoleae

Ayalew,

Mekuria,

Despres

et al. 2024

Animals

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Arcanobacterium hippocoleae is a Gram-positive fastidious bacterium and is occasionally isolated from the reproductive tract of apparently healthy mares (Equus caballus) or from mares with reproductive tract abnormalities. Apart from a few 16S rRNA gene-based GenBank sequences and one recent report on complete genome assembly, detailed genomic sequence and clinical experimental data are not available on the bacterium. Recently, we observed an unusual increase in the detection of the organism from samples associated with mare reproductive failures in Atlantic Canada. Two colony morphotypes (i.e., small, and large) were detected in culture media, which were identified as A. hippocoleae by MALDI-TOF mass spectrometry and 16S rRNA gene sequencing. Here, we report the whole genome sequencing and characterization of the morphotype variants. The genome length of the large phenotypes was between 2.42 and 2.43, and the small phenotype was 1.99 Mbs. The orthologous nucleotide identity between the large colony phenotypes was ~99%, and the large and small colony phenotypes was between 77.86 and 78.52%, which may warrant the classification of the two morphotypes into different species. Phylogenetic analysis based on 16S rRNA genes or concatenated housekeeping genes grouped the small and large colony variants into two different genotypic clusters. The UvrA protein, which is part of the nucleotide excision repair (NER) system, and 3-isopropoylmalate dehydratase small subunit protein expressed by the leuD gene were identified as potential virulence factors in the large and small colony morphotypes, respectively. However, detailed functional studies will be required to determine the exact roles of these and other identified hypothetical proteins in the cellular metabolism and potential pathogenicity of A. hippocoleae in mares.

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Section: Discussionmentioning

confidence: 99%

Genome Sequence Comparisons between Small and Large Colony Phenotypes of Equine Clinical Isolates of Arcanobacterium hippocoleae

Ayalew,

Mekuria,

Despres

et al. 2024

Animals

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“…In addition, our experiments highlighted the potential of compounds for the of treatment of bovine trichomonosis, although we did not assess this directly in infected animals. Acetic acid, chlorhexidine gluconate, and hydrogen peroxide were parasiticidal have been used on skin and mucosal surfaces for various purposes which provides support and rationale for pursuing in vivo treatment studies with these particular compounds (17)(18)(19).…”

Section: Discussionmentioning

confidence: 99%

Assessment of in vitro efficacy for common surface disinfectants and antiseptics against Tritrichomonas foetus trophozoites

Martin,

Kovach,

Moscoso

et al. 2023

Front. Vet. Sci.

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The protozoan Tritrichomonas foetus causes early embryonic death in cattle, there are no legal options for treating this parasite in the United States, and there are few developed protocols for cleaning veterinary and obstetrical equipment that may have been contaminated with trophozoites. In this study, we evaluated bleach, ethanol, acetic acid, chlorhexidine gluconate, and hydrogen peroxide solutions for the ability to kill trophozoites in vitro. Our findings suggested that ethanol and bleach could adequately disinfect tools and equipment. Acetic acid, chlorhexidine, and hydrogen peroxide had applications as surface disinfectants in addition to potential as local topical treatments due to their past uses in veterinary theriogenology. Chlorhexidine gluconate demonstrated trophocidal effects by damaging parasite cell membranes and had the lowest effective concentration 50 (EC50) of any compound tested and was in the micromolar range. These findings, in conjunction with accepted clinical uses of chlorhexidine gluconate suggest that this is a convenient agent for disinfecting equipment. In addition, topical use of chlorhexidine is relatively common, setting the stage for further investigation of this compound as a topical therapeutic option for bovine trichomonosis.

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“…Qiwei Yang, Ph.D.,3 Jack L. Arbiser, MD, PhD,4 Ayman Al-Hendy, MD, PhD3 1 Clinical Pharmacy Department, Faculty of Pharmacy, Ain Shams University, Cairo, Egypt; 2 Center of Postgraduate Medical Education, Warsaw, Poland; 3 Department of Obstetrics and Gynecology, University of Chicago; 4 Emory University, Atlanta, GA.…”

mentioning

confidence: 99%

ADMINISTRATION OF HORMONAL ADD-BACK THERAPY (ABT) COUNTERACTS THE UTERINE VOLUME REDUCING EFFECTS OF ORAL GnRH ANTAGONIST THERAPY

Donnez

Taylor

Catherino

et al. 2021

Fertility and Sterility

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four categories (<1, 1-3, >3-10, and >10 mg/L) according to American Heart Association (AHA) criteria. We used linear mixed models, marginal models, and GEE, as appropriate, adjusting for, age, race, body mass index, education, and cycle of blood draw.RESULTS: There were 1,894 menstrual cycles from 638 women included in the analysis. Compared to CRP <1 mg/L, higher CRP levels were not significantly associated change in continuous menstrual cycle length. However, compared to CRP <1 mg/L, a CRP >10 mg/L was associated with over twice the odds of having long cycles. When evaluating follicular phase length, a CRP of >10 mg/L was associated both with follicular phases that were 2.4 days longer and with over 2.5 times the odds of being long. Compared to CRP <1 mg/L, CRP 1-3 mg/L was significantly associated with a decreased odds of short cycles and short follicular phase, respectively, with a modest increase in follicular length.CONCLUSIONS: Among women age 30-44 attempting to conceive naturally, high levels of CRP are associated with increased odds of a long cycle and longer follicular phase length.IMPACT STATEMENT: Our findings demonstrate a potential pathophysiologic association between chronic inflammation and menstrual cycle characteristics. Further studies are required to further elucidate the mechanism.

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Assessment of uterine luminal pH in mares and the effect of dilute vinegar lavage on uterine luminal pH and endometrial health

Cited by 5 publications

References 34 publications

Genome Sequence Comparisons between Small and Large Colony Phenotypes of Equine Clinical Isolates of Arcanobacterium hippocoleae

Genome Sequence Comparisons between Small and Large Colony Phenotypes of Equine Clinical Isolates of Arcanobacterium hippocoleae

Assessment of in vitro efficacy for common surface disinfectants and antiseptics against Tritrichomonas foetus trophozoites

ADMINISTRATION OF HORMONAL ADD-BACK THERAPY (ABT) COUNTERACTS THE UTERINE VOLUME REDUCING EFFECTS OF ORAL GnRH ANTAGONIST THERAPY

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