Assessment of Vitamin B2 Status in Performance Athletes of Various Types of Sports.

Rokitzki, L; Sagredos, A; Keck, E.; Sauer, Brian C.; Keul, J.

doi:10.3177/jnsv.40.11

Cited by 10 publications

(7 citation statements)

References 14 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…We have tried to determine vitamin B 2 in the presence of blood serum, though we are unable to determine the amount of vitamin B 2 in the blood serum directly because of the very low level (0.11 μg/mL) of vitamin B 2 in blood serum. 61 But from the spectrum of Figure S10 in the Supporting Information, it is evident that energy transfer process is occurring in presence of blood serum, so we can determine vitamin B 2 in the presence of blood serum by the FRET method. Basically there is no difference between the FRET efficiencies in presence of different biomolecules as evident from the almost same fluorescence intensity values of SG/RF system in presence of DNA, RNA, BSA, Lysine and blood serum (cf.…”

Section: ■ Introductionmentioning

confidence: 99%

Fluorescence Resonance Energy Transfer from Sulfonated Graphene to Riboflavin: A Simple Way to Detect Vitamin B₂

Kundu

Nandi

Layek

et al. 2013

ACS Appl. Mater. Interfaces

View full text Add to dashboard Cite

We have prepared sulfonated graphene (SG) by diazonium coupling technique and it has been characterized by UV-vis absorption spectroscopy, Raman spectroscopy, electron microscopy, energy-dispersive spectroscopy (EDS), EDS elemental mapping, X-ray photoelectron spectroscopy (XPS), and FTIR spectroscopy. The photoluminescence (PL) property of SG at different pH (pH 4, 7, and 9.2) has been investigated and SG shows highest PL-intensity and quantum yield at pH 4 compared to those at higher pH and that of GO at pH 4. Due to the strong overlap between the emission spectrum of SG and absorption spectrum of riboflavin (RF, vitamin B2) at pH 4, it has been tactfully used as donor for the fluorescence resonance energy transfer (FRET) process. However, graphene oxide (GO) does not exhibit any FRET with RF at an identical condition due to its much lower quantum yield. We have demonstrated a selective detection of vitamin B2 in presence of nucleic acid (DNA, RNA), protein (BSA), amino acid (Lysine) and other water-soluble vitamins (Becosules, Zevit capsules) based on the spontaneous FRET from PL-active SG (donor) to RF (acceptor). The calibration curve indicates excellent affirmation to detect vitamin B2 using FRET and it is superior to the ordinary fluorescence method of detecting RF in presence of different biomolecules.

show abstract

Section: ■ Introductionmentioning

confidence: 99%

Fluorescence Resonance Energy Transfer from Sulfonated Graphene to Riboflavin: A Simple Way to Detect Vitamin B₂

Kundu

Nandi

Layek

et al. 2013

ACS Appl. Mater. Interfaces

View full text Add to dashboard Cite

show abstract

“…The strength of our study is the use of a well-characterized study population including high-fit and low-fit individuals with a validated difference in VO 2 peak, while previous studies included only a high-fit or low-fit group that received exercise interventions [14][15][16][17]21,28,30] or selected their study population by exercise routines or physical activity questionnaires [20,24,27,44]. Another strength of our study is that we sampled blood the day after the exercise intervention to mimic a situation that allowed us to examine if the recent effects of exercise could interfere with the EGRAC determination on the next day.…”

Section: Discussionmentioning

confidence: 99%

“…However, not all exercise studies confirm these findings [20,21]; one study failed to show a change in vitamin B2 status [20], whereas another study even showed an improved vitamin B2 status following a longer-term exercise intervention [21]. Large observational studies comparing the vitamin B2 status in athletes and recreationally active individuals also show inconsistent results [22][23][24][25][26][27]. Differences in experimental setup, including the selection of the study population [14][15][16][17][18][19][20][21][22][23][24][26][27][28] and the assessment of subjects' fitness levels [24,27] could be of importance.…”

Section: Introductionmentioning

confidence: 99%

“…Large observational studies comparing the vitamin B2 status in athletes and recreationally active individuals also show inconsistent results [22][23][24][25][26][27]. Differences in experimental setup, including the selection of the study population [14][15][16][17][18][19][20][21][22][23][24][26][27][28] and the assessment of subjects' fitness levels [24,27] could be of importance. However, the inconsistent findings could also be related to alterations in the EGR enzymatic activity itself.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

The Effect of a Single Bout of Exercise on Vitamin B2 Status Is Not Different between High- and Low-Fit Females

et al. 2021

View full text Add to dashboard Cite

High-fitness individuals have been suggested to be at risk of a poor vitamin B2 (riboflavin) status due to a potentially higher vitamin B2 demand, as measured by the erythrocyte glutathione reductase (EGR) activation coefficient (EGRAC). Longer-term exercise interventions have been shown to result in a lower vitamin B2 status, but studies are contradictory. Short-term exercise effects potentially contribute to discrepancies between studies but have only been tested in limited study populations. This study investigated if vitamin B2 status, measured by EGRAC, is affected by a single exercise bout in females who differ in fitness levels, and that represents long-term physical activity. At baseline and overnight after a 60-min cycling bout at 70% V·O2peak, EGR activity and EGRAC were measured in 31 young female adults, divided into a high-fit (V·O2peak ≥ 47 mL/kg/min, N = 15) and low-fit (V·O2peak ≤ 37 mL/kg/min, N = 16) group. A single exercise bout significantly increased EGR activity in high-fit and low-fit females (Ptime = 0.006). This response was not affected by fitness level (Ptime*group = 0.256). The effect of exercise on EGRAC was not significant (Ptime = 0.079) and not influenced by EGR activity. The exercise response of EGRAC was not significantly different between high-fit and low-fit females (Ptime*group = 0.141). Thus, a single exercise bout increased EGR activity, but did not affect EGRAC, indicating that vitamin B2 status was not affected. The exercise response on EGRAC and EGR did not differ between high-fit and low-fit females.

show abstract

“…Most cross-sectional studies indicate that nonsupplementing female athletes meet dietary recommendations for riboflavin (Table 7.4) (Kaiserauer et al 1989;Keith et al 1989;Nieman et al 1989;Worme et al 1990;Faber and Benadé 1991;Keith and Alt 1991;Fogelholm et al 1992;Rokitzki et al 1994a;Beals and Manore 1998;Kopp-Woodroffe et al 1999;Beshgetoor and Nichols 2003;Clark et al 2003). However, some studies suggest that female athletes might be at risk for poor riboflavin status due to increased nutrient needs.…”

Section: Dietary Intake and Status Of Riboflavin In Active Adultsmentioning

confidence: 92%

- Fluid and Electrolyte Requirements for Female Athletes

2013

Nutrition and the Female Athlete

View full text Add to dashboard Cite

Assessment of Vitamin B2 Status in Performance Athletes of Various Types of Sports.

Cited by 10 publications

References 14 publications

Fluorescence Resonance Energy Transfer from Sulfonated Graphene to Riboflavin: A Simple Way to Detect Vitamin B₂

Fluorescence Resonance Energy Transfer from Sulfonated Graphene to Riboflavin: A Simple Way to Detect Vitamin B₂

The Effect of a Single Bout of Exercise on Vitamin B2 Status Is Not Different between High- and Low-Fit Females

- Fluid and Electrolyte Requirements for Female Athletes

Contact Info

Product

Resources

About

Assessment of Vitamin B2 Status in Performance Athletes of Various Types of Sports.

Cited by 10 publications

References 14 publications

Fluorescence Resonance Energy Transfer from Sulfonated Graphene to Riboflavin: A Simple Way to Detect Vitamin B2

Fluorescence Resonance Energy Transfer from Sulfonated Graphene to Riboflavin: A Simple Way to Detect Vitamin B2

The Effect of a Single Bout of Exercise on Vitamin B2 Status Is Not Different between High- and Low-Fit Females

- Fluid and Electrolyte Requirements for Female Athletes

Contact Info

Product

Resources

About

Fluorescence Resonance Energy Transfer from Sulfonated Graphene to Riboflavin: A Simple Way to Detect Vitamin B₂

Fluorescence Resonance Energy Transfer from Sulfonated Graphene to Riboflavin: A Simple Way to Detect Vitamin B₂