Assessment of Vitronectin, Soluble Epithelial-Cadherin and TGF-β1 as a Serum Biomarker with Predictive Value for Endometrial and Ovarian Cancers

Turan, Taylan; Torun, Meral; Atalay, Funda; Gönenç, Aymelek

doi:10.4274/tjps.81994

Cited by 5 publications

(5 citation statements)

References 39 publications

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“…It has been pointed out that vitronectin plays an important role in pathophysiological processes and its biosynthesis can be regulated during disease progression. By using ELISA approach, a significant decrease of vitronectin in serum of patients with endometrial and ovarian cancer was observed (Turan et al 2017). Similar reduced concentration in breast cancer samples was figured out, elsewhere (Kim et al 2009).…”

Section: Discussionsupporting

confidence: 75%

“…Similarly as in E-cadherin, vitronectin protein exhibited lower concentration in endothelial cancer patients (ECP) than in control group as seen in Figure 3. Vitronectin is a main plasma glycoprotein with multiple functions including regulation of cell differentiation, proliferation and morphogenesis (cell adhesion molecule) (Turan et al 2017). It also belongs among the major components of the extracellular matrix together with collagens, laminin and fibronectin.…”

Section: Discussionmentioning

confidence: 99%

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Differential Urinary Proteomic Analysis of Endometrial Cancer

Kacířová

Bober²,

Alexovič³

et al. 2019

Physiol Res

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Endometrial cancer is one of the most frequent gynecological malignancies present in more than 95 % of all uterine cancers. In spite of that, screening of such disease is not commonly performed in clinical practice due to enormous costs and relatively low sensitivity. Therefore, developing an effective screening test to diagnose endometrial cancer at early stages is of great importance for the clinical area of investigation. In this work, we applied urinary proteomics (i.e., bottom-up proteomic approach followed by nano HPLC-ESI-MS/MS) in patients with endometrial cancer, with respect to find proteins aimed for the early diagnostics and screening. According to the results, the significant semi-quantitative changes were observed in urinary proteome of treated patients. The proteins that may be pivotal in pathogenesis of endometrial cancer, like cadherin-1 (CDH1), vitronectin (VTN) and basement membrane specific-heparan sulphate proteoglycan core protein (HSPG2) were down-regulated, when compared to the control group. Ultimately, it can be stated that urinary proteomics has a potential for the searching of cancer protein biomarkers based on their altered concentration.

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Section: Discussionsupporting

confidence: 75%

Section: Discussionmentioning

confidence: 99%

Differential Urinary Proteomic Analysis of Endometrial Cancer

Kacířová

Bober²,

Alexovič³

et al. 2019

Physiol Res

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“…Kadowaki et al in their study demonstrated that vitronectin expression was significantly elevated in the serum of early and more advanced breast cancer patients compared to normal controls [18]. Turan et al have proposed vitronectin as a marker in ovarian cancer [19]. Figure 3 shows that the precipitation method provides higher SC of the keratin family proteins and albumin.…”

Section: Resultsmentioning

confidence: 98%

Optimisation of urine sample preparation for shotgun proteomics

2020

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Urine reflects the renal function and urinary and kidney systems, but it may also reflect the presence of cancer in other parts of the body. Urine also has potential for providing prognostic information during therapeutic treatments thanks to non-invasive monitoring. A quick and reproducible protein purification procedure is essential to allow data comparison between proteomic studies in urine biomarker discovery. The article describes a simple, reproducible and cheap sample preparation procedure with a maximum protein yield (400 µg) obtained from only 10 mL of urine utilising cut-off filter desalting and digestion. The reported procedure removes yellowish background coloration residues and thus prevents the errors in spectrophotometric protein concentration determination. Different extraction solvents used in the presented procedure point to the possibility of partial elimination of abundant proteins (albumin and keratin family), as well as to the improvement of the sequence coverage of proteins identified, which helps to reveal changes in the urinary proteome. With this workflow, proteins can be easily obtained on standard laboratory equipment within 3 h. Data are available via ProteomeXchange with identifier PXD019738.

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“…The expression of versican does have an impact on survival in correlation with infiltration depth. In patients with cervical cancer with an infiltration depth greater than 15 mm, low expression of versican predicts poorer survival [ 41 , 42 ].…”

Section: Resultsmentioning

confidence: 99%

“…Their adhesive characteristics play an important role in cell rearrangement, sorting, and migration [ 40 ]. Several studies have shown that lower cadherin expression is associated with extensive tumour invasion and metastasis in endometrial, ovarian, and cervical cancers [ 42 ].…”

Section: Resultsmentioning

confidence: 99%

Changes in the Extracellular Matrix in Endometrial and Cervical Cancer: A Systematic Review

Padežnik

Oleksy

Cokan

et al. 2023

IJMS

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Endometrial and cervical cancers are the two most common gynaecological malignancies and among the leading causes of death worldwide. The extracellular matrix (ECM) is an important component of the cellular microenvironment and plays an important role in developing and regulating normal tissues and homeostasis. The pathological dynamics of the ECM contribute to several different processes such as endometriosis, infertility, cancer, and metastasis. Identifying changes in components of ECM is crucial for understanding the mechanisms of cancer development and its progression. We performed a systematic analysis of publications on the topic of changes in the extracellular matrix in cervical and endometrial cancer. The findings of this systematic review show that matrix metalloproteinases (MMP) play an important role impacting tumour growth in both types of cancer. MMPs degrade various specific substrates (collagen, elastin, fibronectin, aggrecan, fibulin, laminin, tenascin, vitronectin, versican, nidogen) and play a crucial role in the basal membrane degradation and ECM components. Similar types of MMPs were found to be increased in both cancers, namely, MMP-1, MMP-2, MMP-9, and MMP-11. Elevated concentrations of MMP-2 and MMP-9 were correlated with the FIGO stage and are associated with poor prognosis in endometrial cancer, whereas in cervical cancer, elevated concentrations of MMP-9 have been associated with a better outcome. Elevated ADAMTS levels were found in cervical cancer tissues. Elevated disintegrin and metalloproteinase with thrombospondin motifs (ADAMTS) levels were also found in endometrial cancer, but their role is still unclear. Following these findings, this review reports on tissue inhibitors of ECM enzymes, MMPs, and ADAMTS. The present review demonstrates changes in the extracellular matrix in cervical and endometrial cancers and compared their effect on cancer development, progression, and patient prognosis.

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Assessment of Vitronectin, Soluble Epithelial-Cadherin and TGF-β1 as a Serum Biomarker with Predictive Value for Endometrial and Ovarian Cancers

Cited by 5 publications

References 39 publications

Differential Urinary Proteomic Analysis of Endometrial Cancer

Differential Urinary Proteomic Analysis of Endometrial Cancer

Optimisation of urine sample preparation for shotgun proteomics

Changes in the Extracellular Matrix in Endometrial and Cervical Cancer: A Systematic Review

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