Assessment on Antioxidant Potential and Enzyme Activity of Some Economic Resource Plants

Boo, Hee-Ock; Shin, Jeoung-Hwa; Shin, Ji-San; Choung, Eui-Su; Bang, Mi-Ae; Choi, Kyung-Min; Song, Won-Seob

doi:10.7732/kjpr.2012.25.3.349

Cited by 14 publications

(4 citation statements)

References 35 publications

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“…Other studies showed that the scavenger activity of the radical DPPH of the roots of Phragmites communis is very low with high concentrations that comes up 2500 mg/l and gave only a percentage of 7.8±0.32% [Boo et al 2012].…”

Section: Dpph Assaymentioning

confidence: 98%

Heavy Metals Chelating Ability and Antioxidant Activity of Phragmites Australis Stems Extracts

Sellal¹,

Belattar²,

Bouzidi³

2019

J. Ecol. Eng.

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In this study, the ability of hexane (HSE), chloroform (CSE), ethyl acetate (EASE) and methanolic (MSE) stems extracts from Phragmites australis or EDTA (as standard) to chelate iron using ferrozine method or zinc and copper using the murexide method is carried out in vitro. When the increased volumes of the HSE studied were taken from a stock solution of a fixed concentration 1 mg/ml at 25-175 μl for the iron and zinc chelating assay, 1-7 mg/ml for the copper chelating assay gave a significant (p≤ 0.01) activity. The obtained results showed that the HSE have the highest capacity to chelate ferrous ions below the EDTA (standard chelator) with absorbance arrive to the lesser extent 0.24±0.005, 0.04±0.013 which expresses 86% and 97% (compared to the control) of inhibition, respectively. For the murexide chelation, the results obtained also showed that the HSE and EDTA have a good (p≤ 0.01) chelating dose dependent effect towards zinc and copper ions with increased absorbance 0.45±0.02 and 0.42±0.02 with 54% and 56% of inhibition, respectively, for the zinc chelation and 0.66±0.03, 0.13±0.005 represent 44% and 88% for copper chelation. In contrast to the antioxidant capacity, the extract of hexane, ethyl acetate, chloroform and methanol from leaves, stems and roots of the Phragmites australis plant have a very low scavenger effect to the radical DPPH where the maximum inhibition is approximately 13.79%, obtained with the maximum volume. Finally, the HPLC analysis of effective extract (HSE) confirmed the presence of oxalic, citric, malic, succinic, fumaric, formic, acetic, propionic and butyric or iso butyric acid.

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Section: Dpph Assaymentioning

confidence: 98%

Heavy Metals Chelating Ability and Antioxidant Activity of Phragmites Australis Stems Extracts

Sellal¹,

Belattar²,

Bouzidi³

2019

J. Ecol. Eng.

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“…The investigation of the antioxidant activity of natural substances is based on the measuring of the electron donor capacity of DPPH with the ability to inhibit the oxidation by donating electrons in free radicals causing this lipid peroxidation (Boo et al, 2012), that is, free radical are known to be a major factor in biological damages, and DPPH has been used to evaluate the free radical-scavenging activity of natural antioxidants (Yokozawa et al, 1998;Zhu et al, 2001). Active oxygen caused by in vivo metabolism removed by the body's antioxidant system, but excessive free radicals induced stress, causing the lipid peroxidation by combining with unsaturated fatty acids in the cell membrane, and brought intracellular structural and functional damage.…”

Section: Dpph Radical Scavenging Activitymentioning

confidence: 99%

Antioxidant Activity of Several Cabbage (Brassica oleracea L.) Cultivars

Yang¹,

Songzhuzhao²,

Boo³

2015

Korean Journal of Plant Resources

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-Total phenol, flavonoid and antioxidant components of cabbage leaf samples derived from different cultivar were determined. Total phenol compound content showed the highest amount in methanol extracts from 'YR Howol' cultivar (11.72 ㎎/g), followed by 'Harutame' (10.66㎎/g), 'Winstar' (10.34 ㎎/g) and YR Hero (10.20 ㎎/g). The highest amount of total flavonoid content was observed from the methanol extracts of Harutame (5.39 ㎎/g), followed by Winstar (4.28 ㎎/g), Wialhowol (4.10 ㎎/g). The SOD enzyme activity showed a high activity of 'YR Hogeo' cultivar, and the cultivar of 'YR Howol' cultivar showed the lowest activity of SOD. The activity of CAT and APX showed higher values 'Ogane' and 'YR Hogeol' cultivars than the other cultivars. The POD activities showed relatively high values 'Ogane' and 'YR Howol' cultivars compared with other cultivars. The free radical (DPPH) scavenging activity showed lower IC50 values of 'Harutame' (15.71) and 'YR Howol' cultivar (16.88), however methanol extract of 'YR Hero' cultivar (22.49) being the highest. The extracts of all cabbage cultivars in the reaction solution of pH 1.2 could be decomposed nitrite more than 50%. Especially, the cultivar 'YR Hogeol' and 'Ogane' showed a relatively high nitrite scavenging activity for each 60.13% and 57.20% respectively. The IC50 values of antioxidant activity determined by ABTS were lower in 'Harutame' (17.04) and 'YR Howol' cultivar (17.97), and its results observed similar with values obtained from the same extracts by DPPH method. The result of this study suggests that the methanol extract of Brassica oleracea L. contains the high amount of phenolic and higher radical scavenging activities.

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“…Most of the antioxidant compounds in a typical diet are derived from plant sources and belong to various classes of compounds with a wide variety of physical and chemical properties. The main characteristic of an (Prakash et al, 2001;Boo et al, 2012). In response to the increased production of oxygen radicals the capacity of the antioxidant defense system is increased, but in most situations the response is moderate (Foyer et al, 1994).…”

mentioning

confidence: 99%

Antimicrobial Effect, Antioxidant and Tyrosinase Inhibitory Activity of the Extract from Different Parts of Phytolacca americana L.

Boo¹,

Park²,

Woo³

et al. 2015

Korean J. Crop Sci.

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This study was conducted to clarify the antimicrobial effect, antioxidant and tyrosinase inhibitory activities of the biological composition having the Phytolacca americana, and to enhance the natural materials utilization of foods and cosmetics. The antimicrobial activities of the different parts of P. americana were evaluated using the agar diffusion test. The antimicrobial activity of P. americana was relatively high in Malassezia furfur known as a skin fungi and Vibrio parahaemolyticus compared to Escherichia coli and Staphylococcus epidermidis. However, the antimicrobial activity in Vibrio parahaemolyticus did not show at all parts of P. americana. Both the DPPH radical scavenging activity and ABTS radical scavenging activity have been increased with the higher concentration of methanol extract. In particular, leaf extract of P. americana exhibited the highest activity both ABTS radical scavenging activity and DPPH radical scavenging activity. The nitrite scavenging activity was decreased when the pH was changed from pH 1.2 to pH 6.0. The highest nitrite scavenging activity was exhibited from the methanol extract of fruit, followed by root, stem, and leaf at pH 1.2. However, the nitrite scavenging activity at pH of 6.0 was not almost detected. All plant parts of P. americana showed tyrosinase inhibitory activity. The highest activity was found in the stem, and followed by root, leaf, and fruit in order. These tyrosinase inhibitory activity was progressively increased in a concentration-dependent manner. In this experiment on the methanol extracts of different organ from P. americana, we confirmed that the extract of P. americana showed potent tyrosinase inhibitory activity. Taken together, we conjectured that the P. americana had the potent biological activities, therefore this plant having various functional components could be a good material for development into source of natural food additives and cosmetics.

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Assessment on Antioxidant Potential and Enzyme Activity of Some Economic Resource Plants

Abstract: -

Cited by 14 publications

References 35 publications

Heavy Metals Chelating Ability and Antioxidant Activity of Phragmites Australis Stems Extracts

Heavy Metals Chelating Ability and Antioxidant Activity of Phragmites Australis Stems Extracts

Antioxidant Activity of Several Cabbage (Brassica oleracea L.) Cultivars

Antimicrobial Effect, Antioxidant and Tyrosinase Inhibitory Activity of the Extract from Different Parts of Phytolacca americana L.

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