Assessment the Protective Role of Quercetin on Acrylamide-Induced Oxidative Stress in Rats

Abstract

This study aimed to elucidate whether quercetin treatment could modulate acrylamide (ACR)‐induced DNA damage and oxidative changes in rat brain, liver, kidneys and testes tissues. Fifty adult albino rats were divided into five groups. The first group served as normal control, second group received 50 mg/kg quercetin (QTN) and third group received 20 mg/kg ACR. Fourth and fifth groups received dose of ACR along with 25 or 50 mg/kg QTN, respectively. ACR and QTN were given by oral administrations for 30 days. The results showed that, ACR administration induced significant elevation of alanine transferase, aspartate transferase activities, urea, creatinine and Malondialdehyde levels in serum, whereas, Acetylcholine esterase and testosterone levels were reduced after ACR administration. Moreover, ACR treatment increased Glutathione‐S‐transferase, Myeloperoxidase, Glutathione peroxidase activity, 8‐hydroxy deoxyguanosine, tumor necrosis factor‐α and nitric oxide contents in all tissues. QTN significantly improved the previous parameters. It played a role in ameliorating toxic effects of ACR in rats by reducing oxidative stress. Practical Applications Acrylamide was found in various fried, deep fried and oven‐baked foods that are regularly consumed like chips, crisps and bread, also biscuits, crackers and breakfast cereals. Acrylamide exposure led to increase of alanine transferase, aspartate transferase activities, urea, creatinine and Malondialdehyde levels in serum, whereas, Acetylcholine esterase and testosterone levels were reduced. Moreover, ACR treatment increased Glutathione‐S‐transferase, Myeloperoxidase, Glutathione peroxidase activity, 8‐hydroxy deoxyguanosine, tumor necrosis factor‐α and nitric oxide contents in all tissues. Our study revealed the protective role of quercetin on acrylamide‐induced oxidative stress in rats. Quercetin regulate the generation of inflammatory markers and increasing antioxidant enzyme activity in rat liver, kidneys, brain and testes tissues.

Protective effect of Lactobacillus plantarum ATCC8014 on acrylamide-induced oxidative damage in rats

“…Low dose therapeutic group showed intestinal villus rupture. Two high dose groups showed slight intestinal villus rupture the collapse of the antioxidant system in rats, which is manifested by a sharp decrease in antioxidant enzyme (SOD, CAT) activity and the level of antioxidant (GSH) [28][29][30]. At this time, excessive ROS continuously attacked the cells, and the accumulation of lipid peroxides in the cells eventually caused oxidative damage to various tissues of the rats [31,32].…”

Quercetin influences BSA alpha-helical structures of native, ACR- and NaNO2-modified BSAs

“…Some studies indicate the preventive effect of quercetin towards ACR exposure. El-Beltagi et al (68) showed that oral administration of ACR induced elevation of inflammatory markers and oxidative stress parameters in rats, and coadministration of quercetin in dose 25 and 50 mg/kg ameliorated these unfavorable effects, which suggests that quercetin has a protective role in ACR-induced oxidative stress in the animal model. Yang et al (69) also indicated that quercetin had a beneficial effect on the metabolic profile of the serum of ACR-treated rats.…”

Acrylamide: A Neurotoxin and a Hazardous Waste

“…Quercetin enhanced DA and serotonin levels, reduced biomarkers of oxidative stress, restored acetylcholinesterase (AChE) activity in ACR-treated rats. It can move across the BBB and exhibit its therapeutic efficiency [36,68,69]. Other compounds like metformin, minocycline and zolpidem also show similar therapeutic effects to dietary supplements when administered to ACR-treated rats [70][71][72] (Table 2).…”